Construct: ORF ccsbBroadEn_11612
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017499.1_s300c1, BRDN0000395695
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLI (11201)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11201 | POLI | DNA polymerase iota | NM_001351632.2 | 100% | 100% | |
| 2 | human | 11201 | POLI | DNA polymerase iota | NM_007195.2 | 96.6% | 96.6% | 1_75del |
| 3 | human | 11201 | POLI | DNA polymerase iota | NM_001351611.2 | 93.9% | 93.9% | 0_1ins129 |
| 4 | human | 11201 | POLI | DNA polymerase iota | NM_001351612.2 | 93.9% | 93.9% | 0_1ins129 |
| 5 | human | 11201 | POLI | DNA polymerase iota | NM_001351610.1 | 90.9% | 90.9% | 1_75del;114_115ins126 |
| 6 | human | 11201 | POLI | DNA polymerase iota | NM_001351614.2 | 89% | 89% | 0_1ins234 |
| 7 | human | 11201 | POLI | DNA polymerase iota | NM_001351615.2 | 89% | 89% | 0_1ins234 |
| 8 | human | 11201 | POLI | DNA polymerase iota | XM_024451081.1 | 89% | 89% | 0_1ins234 |
| 9 | human | 11201 | POLI | DNA polymerase iota | NM_001351613.1 | 85.9% | 85.9% | 1_75del;559_560ins237 |
| 10 | human | 11201 | POLI | DNA polymerase iota | NM_001351616.1 | 80.2% | 80.2% | 1_75del;114_115ins126;433_434ins237 |
| 11 | human | 11201 | POLI | DNA polymerase iota | NM_001351617.2 | 78.1% | 77.4% | 0_1ins466;16_17insGT |
| 12 | human | 11201 | POLI | DNA polymerase iota | NM_001351618.2 | 78.1% | 77.4% | 0_1ins466;16_17insGT |
| 13 | human | 11201 | POLI | DNA polymerase iota | NM_001351619.2 | 78.1% | 77.4% | 0_1ins466;16_17insGT |
| 14 | human | 11201 | POLI | DNA polymerase iota | NM_001351620.2 | 66.9% | 66.4% | (many diffs) |
| 15 | human | 11201 | POLI | DNA polymerase iota | NR_147257.1 | 63.6% | (many diffs) | |
| 16 | human | 11201 | POLI | DNA polymerase iota | NM_001351621.1 | 52.7% | 50.5% | (many diffs) |
| 17 | human | 11201 | POLI | DNA polymerase iota | XM_011525797.1 | 52.7% | 50.5% | (many diffs) |
| 18 | human | 11201 | POLI | DNA polymerase iota | XM_005258192.4 | 50.7% | 50.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2211
- ORF length:
- 2145
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGA ACTGGCGGAC GTGGGGGCGG CAGCCAGCTC GCAGGGAGTT CATGATCAAG 121 TGTTGCCCAC ACCAAATGCT TCATCCAGAG TCATAGTACA TGTGGATCTG GATTGCTTTT 181 ATGCACAAGT AGAAATGATC TCAAATCCAG AGCTAAAAGA CAAACCTTTA GGGGTTCAAC 241 AGAAATATTT GGTGGTTACC TGCAACTATG AAGCTAGGAA ACTTGGAGTT AAGAAACTTA 301 TGAATGTCAG AGATGCAAAA GAAAAGTGTC CACAGTTGGT ATTAGTTAAT GGAGAAGACC 361 TGACCCGCTA CAGAGAAATG TCTTATAAGG TTACAGAATT ACTGGAAGAA TTTAGTCCAG 421 TTGTTGAGAG ACTTGGATTT GATGAAAATT TTGTGGATCT AACAGAAATG GTTGAGAAGA 481 GACTACAGCA GCTGCAAAGT GATGAACTTT CTGCGGTGAC TGTGTCGGGT CATGTATACA 541 ATAATCAGTC TATAAACCTG CTTGACGTCT TGCACATCAG ACTACTTGTT GGATCTCAGA 601 TTGCAGCAGA GATGCGGGAA GCCATGTATA ATCAGTTGGG GCTCACTGGC TGTGCTGGAG 661 TGGCTTCTAA TAAACTGTTG GCAAAATTAG TTTCTGGTGT CTTTAAACCA AATCAACAAA 721 CAGTCTTATT ACCTGAAAGT TGTCAACATC TTATTCATAG TTTGAATCAC ATAAAGGAAA 781 TACCTGGTAT TGGCTATAAA ACTGCCAAAT GTCTTGAAGC ACTGGGTATC AATAGTGTGC 841 GTGATCTCCA AACCTTTTCA CCCAAAATTT TAGAAAAAGA ATTAGGAATT TCAGTTGCTC 901 AGCGTATCCA AAAGCTCAGT TTTGGAGAGG ATAACTCCCC TGTGATACTC TCAGGACCAC 961 CTCAGTCCTT TAGTGAAGAA GATTCATTTA AAAAATGTTC ATCTGAAGTT GAAGCTAAAA 1021 ATAAGATTGA AGAACTACTT GCTAGTCTTT TAAACAGAGT ATGCCAAGAT GGAAGGAAGC 1081 CTCATACAGT GAGATTAATA ATCCGTCGGT ATTCCTCTGA GAAGCACTAT GGTCGTGAGA 1141 GTCGTCAGTG CCCTATTCCT TCACATGTAA TTCAGAAATT AGGGACAGGA AATTATGATG 1201 TGATGACCCC AATGGTTGAT ATACTTATGA AACTTTTTCG AAATATGGTG AATGTGAAGA 1261 TGCCATTTCA CCTTACCCTT CTAAGTGTGT GCTTCTGCAA CCTTAAAGCA CTAAATACTG 1321 CTAAGAAAGG GCTTATTGAT TATTATTTAA TGCCATCATT ATCAACTACT TCACGCTCTG 1381 GCAAGCACAG TTTTAAAATG AAAGACACTC ATATGGAAGA TTTTCCCAAA GACAAAGAAA 1441 CAAACCGGGA TTTCCTACCA AGTGGAAGAA TTGAAAGTAC AAGAACTAGG GAGTCTCCAC 1501 TAGATACCAC AAATTTTTCT AAAGAAAAAG ACATTAATGA ATTCCCACTC TGTTCACTTC 1561 CTGAAGGTGT TGACCAAGAA GTCTTCAAGC AGCTTCCAGT AGATATTCAA GAAGAAATCC 1621 TTTCTGGAAA ATCTAGGGAA AAATTTCAAG GGAAAGGAAG TGTGAGTTGT CCATTACATG 1681 CCTCTAGAGG AGTATTATCT TTCTTTTCTA AAAAACAAAT GCAAGATATT CCCATAAATC 1741 CTAGAGATCA TTTATCCAGT AGCAAACAGG TATCCTCTGT ATCTCCTTGT GAACCGGGAA 1801 CATCAGGCTT TAATAGCAGT AGTTCTTCTT ACATGTCTAG CCAAAAGGAT TATTCATATT 1861 ATTTAGATAA TAGATTAAAA GATGAACGAA TAAGTCAAGG ACCTAAAGAA CCTCAAGGAT 1921 TCCACTTTAC AAATTCAAAC CCTGCTGTGT CTGCTTTTCA TTCATTTCCA AACTTGCAGA 1981 GTGAGCAACT TTTCTCCAGA AACCACACTA CAGATAGCCA TAAGCAAACA GTAGCAACAG 2041 ACTCTCATGA AGGACTTACA GAAAATAGAG AGCCAGATTC TGTTGATGAG AAAATTACTT 2101 TCCCTTCTGA CATTGATCCT CAAGTTTTCT ATGAACTACC AGAAGCAGTA CAAAAGGAAC 2161 TGCTGGCAGA GTGGAAGAGA GCAGGATCAG ATTTCCACAT TGGACATAAA TACCCAACTT 2221 TCTTGTACAA AGTtggcatt ataagaaagc attgcttatc aatttgttgc aacgaac