Transcript: Human NM_001277403.2

Homo sapiens zinc finger protein 730 (ZNF730), mRNA.

Source:
NCBI, updated 2019-05-01
Taxon:
Homo sapiens (human)
Gene:
ZNF730 (100129543)
Length:
2326
CDS:
150..1661

Additional Resources:

NCBI RefSeq record:
NM_001277403.2
NBCI Gene record:
ZNF730 (100129543)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001277403.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000218427 ACTGGAGAGAAACCCTATAAA pLKO_005 984 CDS 100% 15.000 7.500 Y ZNF443 n/a
2 TRCN0000374174 ACTGGAGAGAAACCCTATAAA pLKO_005 984 CDS 100% 15.000 7.500 Y Zfp97 n/a
3 TRCN0000236731 ACCTTACTACACATAAGATAA pLKO_005 1702 3UTR 100% 13.200 6.600 Y ZNF98 n/a
4 TRCN0000428574 CTGAAGAGAAACCCTACAAAT pLKO_005 1729 3UTR 100% 13.200 6.600 Y ZNF138 n/a
5 TRCN0000096537 CTGGAGAGAAACCCTACAAAT pLKO.1 1405 CDS 100% 13.200 6.600 Y Zfp934 n/a
6 TRCN0000235358 CTGGAGAGAAACCCTACAAAT pLKO_005 1405 CDS 100% 13.200 6.600 Y 2810408B13Rik n/a
7 TRCN0000244342 CTGGAGAGAAACCCTACAAAT pLKO_005 1405 CDS 100% 13.200 6.600 Y EG668616 n/a
8 TRCN0000236733 TCACACTTAGCTCAACATAAA pLKO_005 705 CDS 100% 13.200 6.600 Y ZNF98 n/a
9 TRCN0000013040 CCCAGTTATATGTTCTCATTT pLKO.1 371 CDS 100% 13.200 6.600 Y ZNF208 n/a
10 TRCN0000243782 TGGAGAGAAACCCTATGAATA pLKO_005 1490 CDS 100% 13.200 6.600 Y Zfp977 n/a
11 TRCN0000149073 GCAAAGCCTTTAACCAGTCTT pLKO.1 1018 CDS 100% 4.950 2.475 Y ZNF714 n/a
12 TRCN0000019028 GCCTTTAACCAGTCCTCAATT pLKO.1 1023 CDS 100% 13.200 6.600 Y ZNF92 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001277403.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15167 pDONR223 53.6% 74.1% 36.5% None (many diffs) n/a
2 ccsbBroad304_15167 pLX_304 0% 74.1% 36.5% V5 (not translated due to prior stop codon) (many diffs) n/a
3 ccsbBroadEn_09655 pDONR223 100% 72.8% 63.7% None (many diffs) n/a
4 ccsbBroad304_09655 pLX_304 0% 72.8% 63.7% V5 (many diffs) n/a
5 TRCN0000468487 GAACACCAGATCGGTGGCCCTCAA pLX_317 20.1% 72.8% 63.7% V5 (many diffs) n/a
6 ccsbBroadEn_07157 pDONR223 100% 53.6% 46.3% None (many diffs) n/a
7 ccsbBroad304_07157 pLX_304 0% 53.6% 46.3% V5 (many diffs) n/a
8 TRCN0000475452 TAAAACTTCAACTTGGTTTCCTTC pLX_317 10.2% 53.6% 46.3% V5 (many diffs) n/a
9 ccsbBroadEn_15729 pDONR223 0% 14.3% 13.7% None (many diffs) n/a
10 ccsbBroad304_15729 pLX_304 0% 14.3% 13.7% V5 (many diffs) n/a
11 TRCN0000470492 GCCGACTTGCTCCATGATGCAGCT pLX_317 100% 14.3% 13.7% V5 (many diffs) n/a
12 ccsbBroadEn_11384 pDONR223 100% 13.8% 12.3% None (many diffs) n/a
13 ccsbBroad304_11384 pLX_304 0% 13.8% 12.3% V5 (many diffs) n/a
14 TRCN0000470576 TACATACAGACCTACACGTAGACC pLX_317 100% 13.8% 12.3% V5 (many diffs) n/a
15 ccsbBroadEn_13746 pDONR223 100% 13.2% 12.3% None (many diffs) n/a
16 ccsbBroad304_13746 pLX_304 0% 13.2% 12.3% V5 (many diffs) n/a
17 TRCN0000475669 ATTCGCAGCGAGTTTGTCCGCCGC pLX_317 100% 13.2% 12.3% V5 (many diffs) n/a
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