Transcript: Mouse NM_001347308.1

Mus musculus cyclin-dependent kinase 11B (Cdk11b), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-10
Taxon:
Mus musculus (mouse)
Gene:
Cdk11b (12537)
Length:
2654
CDS:
251..2503

Additional Resources:

NCBI RefSeq record:
NM_001347308.1
NBCI Gene record:
Cdk11b (12537)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001347308.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000361022 GGTCGGTAGGCTGCATATTTG pLKO_005 1980 CDS 100% 13.200 18.480 N Cdk11b n/a
2 TRCN0000361087 TTCAGCGAGTATCCCTATAAC pLKO_005 2141 CDS 100% 13.200 18.480 N Cdk11b n/a
3 TRCN0000023039 CCTACACTCCAGTTGTTGTAA pLKO.1 1893 CDS 100% 5.625 4.500 N Cdk11b n/a
4 TRCN0000361085 ATGGCCTCAAGCACGAATATT pLKO_005 2262 CDS 100% 15.000 10.500 N Cdk11b n/a
5 TRCN0000380353 AGAGGAGAAAGCAGAGATAAA pLKO_005 179 5UTR 100% 13.200 9.240 N CDK11A n/a
6 TRCN0000361023 TGTGGCTCTGAAGCGGTTAAA pLKO_005 1504 CDS 100% 13.200 9.240 N Cdk11b n/a
7 TRCN0000023042 CCTGGGAAGTCAGATATTGAT pLKO.1 2030 CDS 100% 5.625 3.938 N Cdk11b n/a
8 TRCN0000023043 GTGATGAACTACGTGGAACAT pLKO.1 1658 CDS 100% 4.950 3.465 N Cdk11b n/a
9 TRCN0000023041 GCTGAGTCTTCATCAGCAGAA pLKO.1 983 CDS 100% 0.405 0.284 N Cdk11b n/a
10 TRCN0000023040 GCCAGAGTGAAAGAGAAAGAA pLKO.1 512 CDS 100% 5.625 3.375 N Cdk11b n/a
11 TRCN0000006994 CAAGAGGAGAAAGCAGAGATA pLKO.1 177 5UTR 100% 4.950 2.970 N CDK11A n/a
12 TRCN0000006210 CAGATGAAATTGTGGCTCTAA pLKO.1 1494 CDS 100% 4.950 3.465 N CDK11B n/a
13 TRCN0000138934 GAAGAGGAGGAGGAAGAAGAA pLKO.1 1100 CDS 100% 4.950 2.475 Y PTMA n/a
14 TRCN0000165520 GAGGAGGAAGAAGAGGAGAAA pLKO.1 1106 CDS 100% 4.950 2.475 Y AP5B1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001347308.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14570 pDONR223 69.3% 81.8% 59.3% None (many diffs) n/a
2 ccsbBroad304_14570 pLX_304 0% 81.8% 59.3% V5 (not translated due to prior stop codon) (many diffs) n/a
3 ccsbBroadEn_13742 pDONR223 100% 46.1% 48% None (many diffs) n/a
4 ccsbBroad304_13742 pLX_304 0% 46.1% 48% V5 (many diffs) n/a
5 TRCN0000475614 GCGTCAAACGAAAACCATTCTATA pLX_317 10.6% 46.1% 48% V5 (many diffs) n/a
6 TRCN0000467626 TAGTGACAATCAACTTACAGCGAA pLX_317 38.1% 29.2% .6% V5 (not translated due to prior stop codon) (many diffs) n/a
7 ccsbBroadEn_14571 pDONR223 100% 29.2% .6% None (many diffs) n/a
8 ccsbBroad304_14571 pLX_304 0% 29.2% .6% V5 (not translated due to prior stop codon) (many diffs) n/a
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