Construct: ORF ccsbBroad304_14570

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018898.1_s304c1, BRDN0000459382
Derived from:
ccsbBroadEn_14570
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
CDK11B (984)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14570
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 984 CDK11B cyclin dependent kinase 11B NM_033486.3 99.1% 66.1% (many diffs)
2 human 984 CDK11B cyclin dependent kinase 11B XM_017002925.2 97.9% 65.3% (many diffs)
3 human 728642 CDK11A cyclin dependent kinase 11A NM_024011.4 97.8% 65.2% (many diffs)
4 human 984 CDK11B cyclin dependent kinase 11B NM_001291345.2 97.8% 64.8% (many diffs)
5 human 984 CDK11B cyclin dependent kinase 11B NM_001787.3 97.5% 65% (many diffs)
6 human 984 CDK11B cyclin dependent kinase 11B XM_017002926.2 96.7% 64% (many diffs)
7 human 728642 CDK11A cyclin dependent kinase 11A NM_033529.4 96.6% 64% (many diffs)
8 human 984 CDK11B cyclin dependent kinase 11B XM_011542490.3 96.4% 64.3% (many diffs)
9 human 984 CDK11B cyclin dependent kinase 11B XM_011542491.2 96.4% 64.3% (many diffs)
10 human 984 CDK11B cyclin dependent kinase 11B XM_011542492.2 96.4% 64.3% (many diffs)
11 human 984 CDK11B cyclin dependent kinase 11B XM_006711065.4 96.3% 63.7% (many diffs)
12 human 728642 CDK11A cyclin dependent kinase 11A NM_001313982.2 95.5% 63.3% (many diffs)
13 human 984 CDK11B cyclin dependent kinase 11B XM_011542493.3 95.2% 63% (many diffs)
14 human 728642 CDK11A cyclin dependent kinase 11A NM_001313896.2 95.1% 63% (many diffs)
15 human 984 CDK11B cyclin dependent kinase 11B NM_033489.3 94.4% 61.3% (many diffs)
16 human 984 CDK11B cyclin dependent kinase 11B XM_017002928.2 93% 60.3% (many diffs)
17 human 984 CDK11B cyclin dependent kinase 11B XM_011542494.3 91.9% 59.7% (many diffs)
18 human 984 CDK11B cyclin dependent kinase 11B XM_017002927.2 91.9% 59.7% (many diffs)
19 human 984 CDK11B cyclin dependent kinase 11B XM_024451174.1 91.9% 59.7% (many diffs)
20 human 984 CDK11B cyclin dependent kinase 11B XM_011542495.3 90.7% 58.4% (many diffs)
21 human 728642 CDK11A cyclin dependent kinase 11A NR_132739.2 73% (many diffs)
22 human 728642 CDK11A cyclin dependent kinase 11A NR_132740.2 72.1% (many diffs)
23 human 984 CDK11B cyclin dependent kinase 11B NM_033490.3 71.5% 38.3% (many diffs)
24 human 984 CDK11B cyclin dependent kinase 11B XM_024451184.1 70.4% 37.7% (many diffs)
25 human 984 CDK11B cyclin dependent kinase 11B NM_033487.3 66.6% 33.8% (many diffs)
26 human 984 CDK11B cyclin dependent kinase 11B XR_002958171.1 61.4% (many diffs)
27 human 984 CDK11B cyclin dependent kinase 11B XR_002958173.1 61.4% (many diffs)
28 human 984 CDK11B cyclin dependent kinase 11B XM_024451190.1 50.2% 14.3% (many diffs)
29 human 984 CDK11B cyclin dependent kinase 11B XR_002958172.1 47.1% (many diffs)
30 human 984 CDK11B cyclin dependent kinase 11B XR_002958174.1 47.1% (many diffs)
31 mouse 12537 Cdk11b cyclin-dependent kinase 11B NM_007661.3 86.5% 64% (many diffs)
32 mouse 12537 Cdk11b cyclin-dependent kinase 11B XM_011250179.2 86.5% 64% (many diffs)
33 mouse 12537 Cdk11b cyclin-dependent kinase 11B XM_006538508.2 86.4% 63.9% (many diffs)
34 mouse 12537 Cdk11b cyclin-dependent kinase 11B XM_017319929.1 86.4% 63.9% (many diffs)
35 mouse 12537 Cdk11b cyclin-dependent kinase 11B NM_001347308.1 81.8% 59.3% (many diffs)
36 mouse 12537 Cdk11b cyclin-dependent kinase 11B XM_006538511.2 81.8% 59.3% (many diffs)
37 mouse 12537 Cdk11b cyclin-dependent kinase 11B XM_017319930.1 81.8% 59.3% (many diffs)
38 mouse 12537 Cdk11b cyclin-dependent kinase 11B XM_017319931.1 81.7% 59.1% (many diffs)
39 mouse 12537 Cdk11b cyclin-dependent kinase 11B XR_001784083.1 75.6% (many diffs)
40 mouse 12537 Cdk11b cyclin-dependent kinase 11B XR_001784084.1 74.9% (many diffs)
41 mouse 12537 Cdk11b cyclin-dependent kinase 11B XM_006538512.3 61.4% 36.4% (many diffs)
42 mouse 12537 Cdk11b cyclin-dependent kinase 11B XM_017319932.1 61.4% 36.4% (many diffs)
43 mouse 12537 Cdk11b cyclin-dependent kinase 11B XM_017319933.1 48.3% 22.1% (many diffs)
44 mouse 12537 Cdk11b cyclin-dependent kinase 11B XM_017319934.1 48.3% 22.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1647
ORF length:
1578
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gggtgatgaa aaggactctt ggaaagtgaa aactttagat gaaattcttc
121aggaaaagaa acgaaggaag gaacaagagg agaaagcaga gataaaacgc ttaaaaaatt
181ctgatgaccg ggattccaag cgggattccc ttgaggaggg ggagctgaga gatcaccgca
241tggagatcac aataaggaac tccccgtata gaagagaaga ctctatggaa gacagaggag
301aagaagatga ttctttggcc atcaaaccac cccagcaaat gtctcggaaa gaaaaagctc
361atcacagaaa agatgaaaag agaaaagaga aacgtaggca tcgtagccat tcagcagaag
421gggggaagca tgctagagtg aaagaaaaag aaagagagca cgaacgtcgg aaacggcatc
481gagaagaaca ggataaagct cgccgggaat gggaaagaca gaagagaagg gagatggcaa
541gggagcattc caggagagaa agggaccgct tggagcagtt agaaaggaag cgggagcggg
601agcgcaagat gcgggagcag cagaaggagc agcgggagca gaaggagcgc gagcggcggg
661cggaggagcg gcgcaaggag cgggaggccc gcagggaagt gtctgcacat caccgaacga
721tgagagagga ctacagcgac aaagtgaaag ccagccactg gagtcgcagc ccgcctcggc
781cgccgcggga gcggttcgag ttgggagacg gccgggaagc cagtaaaaga gagaaaatgg
841aagaaaggga cctgctgtcc gacttacagg acatcagcga cagcgagagg aagaccagct
901cggccgagtc ctcgtcagca gaatcaggct caggttctga ggaagaagag gaggaggagg
961aagaggagga ggaggaaggg agcaccagtg aagaatcaga ggaggaggag gaggaagagg
1021aagaggagga ggaggagacc ggcagcaact ctgaggaggc atcagagcag tctgccgaag
1081aagtaagtga ggaagaaatg agtgaagatg aagaacgaga aaatgaaaac cacctcttgg
1141ttgttccaga gtcacggttc gaccgagatt ccggggagag tgaagaagca gaggaagaag
1201tgggtgaggg aacgccgcag agcagcgccc tgacagaggg cgactatgtg cccgactccc
1261ctgccctgtc gcccatcgag ctcaagcagg agctgcccaa gtacctgccg gccctgcagg
1321gctgccggag cgtcgaggag ttccagtgcc tgaacaggat cgaggagggc acctatggag
1381tggtctacag agcaaaagac aagaaaacag atgaaattgt ggctctaaag cggctgaaga
1441tggagaagga gaaggagggc ttcccgatca cgtcgctgag ggagatcaac accatcctca
1501aggcccagca tcccaacatc gtcaccgtta gagagattgt ggtgggcagc aacatggaca
1561agatctacat cgtgatgaac tatgtggagc acgacctcaa gagcctgatg gagaccagaa
1621aacagcccct tcctgccaag gggaggtgaa gacccctgag aatccaggtt ggtgcgtggg
1681ggtgaaacac ctccacgaca aactgatccc tgcaccgtga cctcaagacg tccacctggc
1741tgctgagcca cgccggcatc ctcaaggtgg gtgacttcgg gctggcgcgg gagtacggat
1801cccctctgaa ggcctacacc ccggtcgtgg tgaccctgtg gtaccgcgcc ccagagctgc
1861tgcttggtgc caaggaatac tccacggccg tggacatgtg gtcagtgggt tgcatcttcg
1921gggagctgct gactcagaag cctctgttcc ccgggaagtc agaaatcgat cagatcaaca
1981aggtgttcaa ggatctgggg acccctagtg agaaaatctg gcccggctac agcgagctcc
2041cagcagtcaa gaagatgacc ttcagcgagc acccctacaa caacctccgc aagcgcttcg
2101gggctctgct ctcagaccag ggcttcgacc tcatgaacaa gttcctgacc tacttccccg
2161ggaggaggat cagcgctgag gacggcctca agcatgagta tttccgcgag acccccctcc
2221ccatcgaccc ctccatgttc cccacgtggc ccgccaagag cgagcagcag cgtgtgaagc
2281ggggcaccag cccgaggccc cctgagggag gcctgggcta cagccagctg ggtgacgacg
2341acctgaagga gacgggcttc caccttacca ccacgaacca gggggcctct gccgcgggcc
2401ccggcttcag cctcaagttc ttgccaactt tcttgtacaa agtggttggt aagcctatcc
2461ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
2521ttaagtcgac aatcaacctc tggatta

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