Transcript: Human NM_001369761.1

Homo sapiens zinc finger protein 28 (ZNF28), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
ZNF28 (7576)
Length:
4676
CDS:
122..511

Additional Resources:

NCBI RefSeq record:
NM_001369761.1
NBCI Gene record:
ZNF28 (7576)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001369761.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000421076 AGATCTTTGGTCACAATACAT pLKO_005 1072 3UTR 100% 5.625 3.938 N ZNF28 n/a
2 TRCN0000425574 CATCGTAGATCTCACATTGAT pLKO_005 1020 3UTR 100% 5.625 3.938 N ZNF28 n/a
3 TRCN0000159780 GTCACAAATCACAGCTTGAAA pLKO.1 3013 3UTR 100% 5.625 3.938 N ZNF28 n/a
4 TRCN0000444783 GCGCTTCATACTGCAGACAAA pLKO_005 1110 3UTR 100% 4.950 3.465 N ZNF28 n/a
5 TRCN0000433076 CAACACCTTCCATCACAATTC pLKO_005 2498 3UTR 100% 10.800 6.480 N ZNF28 n/a
6 TRCN0000158874 GTAATGAATGTGGCATAGTTT pLKO.1 2905 3UTR 100% 5.625 3.375 N ZNF28 n/a
7 TRCN0000158444 CAAATGTGAAGACTGTGACAA pLKO.1 2984 3UTR 100% 4.950 2.970 N ZNF28 n/a
8 TRCN0000159031 GACAAAGTTGTCAGTCACAAA pLKO.1 3000 3UTR 100% 4.950 2.970 N ZNF28 n/a
9 TRCN0000158709 GCAAGTCATCATAGTCTTCAT pLKO.1 2946 3UTR 100% 4.950 2.970 N ZNF28 n/a
10 TRCN0000147524 GAGAGTGGCAAATCCTTTAAT pLKO.1 894 3UTR 100% 15.000 7.500 Y ZNF321P n/a
11 TRCN0000413960 CACACTGGAGAGAAGCCTTAC pLKO_005 1536 3UTR 100% 6.000 3.000 Y Zfp612 n/a
12 TRCN0000016730 CTGGAGAGAAACCTTATGAAT pLKO.1 1372 3UTR 100% 5.625 2.813 Y ZNF345 n/a
13 TRCN0000147302 GTCGCAAATCACATCTTGAAA pLKO.1 1417 3UTR 100% 5.625 2.813 Y ZNF321P n/a
14 TRCN0000015887 GTGAAGAATGTGACAAAGTTT pLKO.1 1141 3UTR 100% 5.625 2.813 Y ZNF702P n/a
15 TRCN0000158848 GAGAAACCTTACAAATGTGAT pLKO.1 2805 3UTR 100% 4.950 2.475 Y ZNF28 n/a
16 TRCN0000143454 GCAAAGCGTTTACTTCACATT pLKO.1 3499 3UTR 100% 4.950 2.475 Y ZNF468 n/a
17 TRCN0000141972 GCACAACATCAGAGAGTTCAT pLKO.1 2022 3UTR 100% 4.950 2.475 Y ZNF468 n/a
18 TRCN0000140423 GCACGCCATCATAGACTTCAT pLKO.1 1602 3UTR 100% 4.950 2.475 Y ZNF468 n/a
19 TRCN0000150214 GTAATGAATGTGGCAAGGTTT pLKO.1 1309 3UTR 100% 4.950 2.475 Y ZNF813 n/a
20 TRCN0000141128 CCTCAGTTTCAACATCCCAAA pLKO.1 754 3UTR 100% 4.050 2.025 Y ZNF468 n/a
21 TRCN0000162177 CAAATGTGATGAGTGTGGCAA pLKO.1 2816 3UTR 100% 2.640 1.320 Y ZNF28 n/a
22 TRCN0000021907 GCTGGAGAATTATAGGAACCT pLKO.1 232 CDS 100% 2.640 1.320 Y ZNF765 n/a
23 TRCN0000142023 GTAAGGTTTGTGACAAGGCTT pLKO.1 1477 3UTR 100% 2.640 1.320 Y ZNF468 n/a
24 TRCN0000420675 GATGTGATGCTGGAGAATTTC pLKO_005 224 CDS 100% 13.200 6.600 Y Zfp874a n/a
25 TRCN0000264189 CAAGTAGCTGGGACTACAGGA pLKO_005 363 CDS 100% 2.640 1.320 Y LINC01098 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001369761.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14339 pDONR223 100% 43.8% 2.3% None (many diffs) n/a
2 ccsbBroad304_14339 pLX_304 0% 43.8% 2.3% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000478280 ACGAGTTCACTGTCATGACCAACC pLX_317 100% 43.8% 2.3% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_08581 pDONR223 100% 16.5% 14.1% None (many diffs) n/a
5 ccsbBroad304_08581 pLX_304 0% 16.5% 14.1% V5 (many diffs) n/a
6 TRCN0000476486 AGTATTTAAATCGATGTCTATAAC pLX_317 30.4% 16.5% 14.1% V5 (many diffs) n/a
7 ccsbBroadEn_05120 pDONR223 100% 15.2% 12.4% None (many diffs) n/a
8 ccsbBroad304_05120 pLX_304 0% 15.2% 12.4% V5 (many diffs) n/a
9 TRCN0000475531 CGCGGTATGATTAGCATATCCGAT pLX_317 14.1% 15.2% 12.4% V5 (many diffs) n/a
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