Transcript: Human NR_147249.2

Homo sapiens CASP8 and FADD like apoptosis regulator (CFLAR), transcript variant 24, non-coding RNA.

Source:
NCBI, updated 2019-09-15
Taxon:
Homo sapiens (human)
Gene:
CFLAR (8837)
Length:
7929
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_147249.2
NBCI Gene record:
CFLAR (8837)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_147249.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000364098 CCAGATCAACTGGATTTATTA pLKO_005 473 3UTR 100% 15.000 21.000 N CFLAR n/a
2 TRCN0000320671 ATCTCAGTATGCATGGTATAT pLKO_005 885 3UTR 100% 13.200 18.480 N CFLAR n/a
3 TRCN0000320749 TTGGTGAGGATTTGGATAAAT pLKO_005 336 3UTR 100% 15.000 10.500 N CFLAR n/a
4 TRCN0000378209 ACATGGGCCGAGGCAAGATAA pLKO_005 393 3UTR 100% 13.200 9.240 N CFLAR n/a
5 TRCN0000007230 GCTCCATAATGGGAGAAGTAA pLKO.1 643 3UTR 100% 5.625 3.938 N CFLAR n/a
6 TRCN0000007231 GCATCACATCAGGAGGATGTT pLKO.1 1036 3UTR 100% 0.495 0.347 N CFLAR n/a
7 TRCN0000007232 CACTCTGAGAAAGAAACTTAT pLKO.1 2283 3UTR 100% 13.200 7.920 N CFLAR n/a
8 TRCN0000134155 CCTTCCTTACACCTTATACAA pLKO.1 4676 3UTR 100% 5.625 2.813 Y FSIP2 n/a
9 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 2349 3UTR 100% 4.950 2.475 Y CFLAR n/a
10 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 2349 3UTR 100% 4.950 2.475 Y C19orf31 n/a
11 TRCN0000155229 GATCAAGACCATCCTGGCTAA pLKO.1 3281 3UTR 100% 4.050 2.025 Y INTS7 n/a
12 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 2347 3UTR 100% 4.950 2.475 Y ERN2 n/a
13 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 2347 3UTR 100% 4.950 2.475 Y P3H4 n/a
14 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 2347 3UTR 100% 4.950 2.475 Y P3H4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_147249.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02030 pDONR223 100% 16.1% None (many diffs) n/a
2 ccsbBroad304_02030 pLX_304 0% 16.1% V5 (many diffs) n/a
3 TRCN0000466010 GCCCTAAGGACAATAGACCAGTAT pLX_317 22.8% 16.1% V5 (many diffs) n/a
4 ccsbBroadEn_13802 pDONR223 100% 8% None (many diffs) n/a
5 ccsbBroad304_13802 pLX_304 0% 8% V5 (many diffs) n/a
6 TRCN0000470799 AAGCCATAAGTTAGAAACAAACCG pLX_317 52.2% 8% V5 (many diffs) n/a
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