Construct: ORF TRCN0000477053
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008523.1_s317c1
- Derived from:
- ccsbBroadEn_11738
- DNA Barcode:
- TCTAGTTTCTTACTTTTTGCCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BRD4 (23476)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477053
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23476 | BRD4 | bromodomain containing 4 | NM_001330384.2 | 100% | 100% | |
| 2 | human | 23476 | BRD4 | bromodomain containing 4 | NM_014299.2 | 90.8% | 90.8% | 2159_2159delGinsCT;2161C>T;2166_2166delCins216 |
| 3 | human | 23476 | BRD4 | bromodomain containing 4 | XM_011527856.3 | 90.8% | 90.8% | 2159_2159delGinsCT;2161C>T;2166_2166delCins216 |
| 4 | human | 23476 | BRD4 | bromodomain containing 4 | NM_058243.2 | 56.5% | 52.2% | (many diffs) |
| 5 | human | 23476 | BRD4 | bromodomain containing 4 | XM_011527854.2 | 56.5% | 52.2% | (many diffs) |
| 6 | mouse | 57261 | Brd4 | bromodomain containing 4 | NM_198094.2 | 81.5% | 87.4% | (many diffs) |
| 7 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524695.3 | 81.4% | 87.3% | (many diffs) |
| 8 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524691.1 | 50.6% | 50.1% | (many diffs) |
| 9 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524690.2 | 50.6% | 50.1% | (many diffs) |
| 10 | mouse | 57261 | Brd4 | bromodomain containing 4 | NM_020508.4 | 49.4% | 48.9% | (many diffs) |
| 11 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524688.1 | 49.4% | 48.9% | (many diffs) |
| 12 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524689.2 | 49.4% | 48.9% | (many diffs) |
| 13 | mouse | 57261 | Brd4 | bromodomain containing 4 | NM_001286630.1 | 49.4% | 48.9% | (many diffs) |
| 14 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524683.2 | 49.4% | 48.9% | (many diffs) |
| 15 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524684.2 | 49.4% | 48.9% | (many diffs) |
| 16 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524685.1 | 49.4% | 48.9% | (many diffs) |
| 17 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524686.1 | 49.4% | 48.9% | (many diffs) |
| 18 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524687.2 | 49.4% | 48.9% | (many diffs) |
| 19 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524693.2 | 39.4% | 38.7% | (many diffs) |
| 20 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_011246543.2 | 39.4% | 38.7% | (many diffs) |
| 21 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_017317588.1 | 39.4% | 38.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2451
- ORF length:
- 2382
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctgcggag agcggccctg ggacgagatt gagaaatctg ccagtaatgg 121 gggatggact agaaacttcc caaatgtcta caacacaggc ccaggcccaa ccccagccag 181 ccaacgcagc cagcaccaac cccccgcccc cagagacctc caaccctaac aagcccaaga 241 ggcagaccaa ccaactgcaa tacctgctca gagtggtgct caagacacta tggaaacacc 301 agtttgcatg gcctttccag cagcctgtgg atgccgtcaa gctgaacctc cctgattact 361 ataagatcat taaaacgcct atggatatgg gaacaataaa gaagcgcttg gaaaacaact 421 attactggaa tgctcaggaa tgtatccagg acttcaacac tatgtttaca aattgttaca 481 tctacaacaa gcctggagat gacatagtct taatggcaga agctctggaa aagctcttct 541 tgcaaaaaat aaatgagcta cccacagaag aaaccgagat catgatagtc caggcaaaag 601 gaagaggacg tgggaggaaa gaaacaggga cagcaaaacc tggcgtttcc acggtaccaa 661 acacaactca agcatcgact cctccgcaga cccagacccc tcagccgaat cctcctcctg 721 tgcaggccac gcctcacccc ttccctgccg tcaccccgga cctcatcgtc cagacccctg 781 tcatgacagt ggtgcctccc cagccactgc agacgccccc gccagtgccc ccccagccac 841 aacccccacc cgctccagct ccccagcccg tacagagcca cccacccatc atcgcggcca 901 ccccacagcc tgtgaagaca aagaagggag tgaagaggaa agcagacacc accaccccca 961 ccaccattga ccccattcac gagccaccct cgctgccccc ggagcccaag accaccaagc 1021 tgggccagcg gcgggagagc agccggcctg tgaaacctcc aaagaaggac gtgcccgact 1081 ctcagcagca cccagcacca gagaagagca gcaaggtctc ggagcagctc aagtgctgca 1141 gcggcatcct caaggagatg tttgccaaga agcacgccgc ctacgcctgg cccttctaca 1201 agcctgtgga cgtggaggca ctgggcctac acgactactg tgacatcatc aagcacccca 1261 tggacatgag cacaatcaag tctaaactgg aggcccgtga gtaccgtgat gctcaggagt 1321 ttggtgctga cgtccgattg atgttctcca actgctataa gtacaaccct cctgaccatg 1381 aggtggtggc catggcccgc aagctccagg atgtgttcga aatgcgcttt gccaagatgc 1441 cggacgagcc tgaggagcca gtggtggccg tgtcctcccc ggcagtgccc cctcccacca 1501 aggttgtggc cccgccctca tccagcgaca gcagcagcga tagctcctcg gacagtgaca 1561 gttcgactga tgactctgag gaggagcgag cccagcggct ggctgagctc caggagcagc 1621 tcaaagccgt gcacgagcag cttgcagccc tctctcagcc ccagcagaac aaaccaaaga 1681 aaaaggagaa agacaagaag gaaaagaaaa aagaaaagca caaaaggaaa gaggaagtgg 1741 aagagaataa aaaaagcaaa gccaaggaac ctcctcctaa aaagacgaag aaaaataata 1801 gcagcaacag caatgtgagc aagaaggagc cagcgcccat gaagagcaag ccccctccca 1861 cgtatgagtc ggaggaagag gacaagtgca agcctatgtc ctatgaggag aagcggcagc 1921 tcagcttgga catcaacaag ctccccggcg agaagctggg ccgcgtggtg cacatcatcc 1981 agtcacggga gccctccctg aagaattcca accccgacga gattgaaatc gactttgaga 2041 ccctgaagcc gtccacactg cgtgagctgg agcgctatgt cacctcctgt ttgcggaaga 2101 aaaggaaacc tcaagctgag aaagttgatg tgattgccgg ctcctccaag atgaagggct 2161 tctcgtcctc agagtcggag agctccagtg agtccagctc ctctgacagc gaagactccg 2221 aaacagcttt cTGCACCAGT GGAGACTTCG TGTCCCCAGG GCCTTCCCCG TATCACAGTC 2281 ACGTGCAGTG CGGCCGCTTC AGGGAGATGC TTCGCTGGTT TCTGGTGGAT GTGGAGCAGA 2341 CTGCAGCTGG CCAGCCGCAT CGCCAGTCTG CTGCTGGCCC TGCCATCACC TGGGCCCCAG 2401 CCATTGCCTA CCCCTCCCCA GAGTGTGCTC GTTGCTGTGT TGGCTGCTCC TTGCCAACTT 2461 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2521 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2581 CTTGTGGAAA GGACGATCTA GTTTCTTACT TTTTGCCGAC ACGCGTTAAG TCgacaatca 2641 acctctggat tacaaaattt gtgaaagatt