Vector: pLKO_005

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Vector Information:

• Vector Name: pLKO_005
• Alias: pLKO.5, pLKO_TRC005
• Pol II Cassette 1: PGK-PuroR
• Pol II Cassette 2: n/a
• Pol III Promoter: constitutive hU6
• Pol III Insert: 1.9 KB Stuffer
• Selection Marker: PuroR
• Visible Reporter: n/a
• Cloning Site: n/a

Sequence Data Files

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	GenBank	FASTA	SnapGene	Revision Notes
1	GB	FASTA	DNA	2017-04-12: Sequence corrected; changes unlikely to have functional consequences
2	GB	FASTA	DNA	(original version)

Control Clones

	Clone ID	Target Seq	Target Gene ID	Target Gene Symbol	Taxon	Target Region	Notes	Addgene
1	TRCN0000231745	TGACCCTGAAGTTCATCTGCA	GFP	GFP	CONTROL	CDS		n/a
2	TRCN0000231759	GACCACATGAAGCAGCACGAC	GFP	GFP	CONTROL	CDS		n/a
3	TRCN0000231762	CCACATGAAGCAGCACGACTT	GFP	GFP	CONTROL	CDS		n/a
4	TRCN0000231753	ACAACAGCCACAACGTCTATA	GFP	GFP	CONTROL	CDS		n/a
5	TRCN0000231746	TGCCCGACAACCACTACCTGA	GFP	GFP	CONTROL	CDS		n/a
6	TRCN0000231747	TACAACAGCCACAACGTCTAT	GFP	GFP	CONTROL	CDS		n/a
7	TRCN0000231750	CAACAGCCACAACGTCTATAT	GFP	GFP	CONTROL	CDS		n/a
8	TRCN0000231764	CTCTCGGCATGGACGAGCTGT	GFP	GFP	CONTROL	CDS		n/a
9	TRCN0000231760	GAACGGCATCAAGGTGAACTT	GFP	GFP	CONTROL	CDS		n/a
10	TRCN0000231751	CGGCATGGACGAGCTGTACAA	GFP	GFP	CONTROL	CDS		n/a
11	TRCN0000231754	CTATATCATGGCCGACAAGCA	GFP	GFP	CONTROL	CDS		n/a
12	TRCN0000231757	CTACGGCAAGCTGACCCTGAA	GFP	GFP	CONTROL	CDS		n/a
13	TRCN0000231748	CGGGATCACTCTCGGCATGGA	GFP	GFP	CONTROL	CDS		n/a
14	TRCN0000231744	AGTACAACTACAACAGCCACA	GFP	GFP	CONTROL	CDS		n/a
15	TRCN0000231761	CGACGTAAACGGCCACAAGTT	GFP	GFP	CONTROL	CDS		n/a
16	TRCN0000231749	CGACCACATGAAGCAGCACGA	GFP	GFP	CONTROL	CDS		n/a
17	TRCN0000231758	GCGCGATCACATGGTCCTGCT	GFP	GFP	CONTROL	CDS		n/a
18	TRCN0000231752	TCTCGGCATGGACGAGCTGTA	GFP	GFP	CONTROL	CDS		n/a
19	TRCN0000231765	CCTACGGCGTGCAGTGCTTCA	GFP	GFP	CONTROL	CDS		n/a
20	TRCN0000231763	CCCGACCACATGAAGCAGCAC	GFP	GFP	CONTROL	CDS		n/a
21	TRCN0000231705	GCCACAACATCGAGGACGGCA	GFP	GFP	CONTROL	CDS		n/a
22	TRCN0000231756	ACGTCTATATCATGGCCGACA	GFP	GFP	CONTROL	CDS		n/a
23	TRCN0000231755	GCGACGTAAACGGCCACAAGT	GFP	GFP	CONTROL	CDS		n/a
24	TRCN0000231721	TGTCCGGTTATGTAAACAATC	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
25	TRCN0000231697	TCACAGAATCGTCGTATGCAG	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
26	TRCN0000231728	GAGTACTTCGAAATGTCCGTT	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
27	TRCN0000231729	GAATGTTTACTACACTCGGAT	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
28	TRCN0000231736	CACTTACGCTGAGTACTTCGA	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
29	TRCN0000231707	CACTCGGATATTTGATATGTG	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
30	TRCN0000231695	ATCACAGAATCGTCGTATGCA	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
31	TRCN0000231718	AGTCAAGTAACAACCGCGAAA	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
32	TRCN0000231730	AGAATCGTCGTATGCAGTGAA	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
33	TRCN0000231740	TGAGTACTTCGAAATGTCCGT	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
34	TRCN0000231734	TCTACTGGTCTGCCTAAAGGT	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
35	TRCN0000231739	AGTACTTCGAAATGTCCGTTC	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
36	TRCN0000231733	ACTTACGCTGAGTACTTCGAA	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
37	TRCN0000231742	ACGCTGAGTACTTCGAAATGT	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
38	TRCN0000231732	ACACTCGGATATTTGATATGT	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
39	TRCN0000231693	CTTCGAAATGTCCGTTCGGTT	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
40	TRCN0000231741	GCTGAGTACTTCGAAATGTCC	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
41	TRCN0000231719	GCGGTTGCCAAGAGGTTCCAT	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
42	TRCN0000231731	GCGCCATTCTATCCGCTGGAA	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
43	TRCN0000231727	GTTGTGTTTGTGGACGAAGTA	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
44	TRCN0000231720	GAATCGTCGTATGCAGTGAAA	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
45	TRCN0000231715	CGCTGAGTACTTCGAAATGTC	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
46	TRCN0000231714	CAGAATCGTCGTATGCAGTGA	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
47	TRCN0000231737	ATGTTTACTACACTCGGATAT	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
48	TRCN0000231699	CAAATCACAGAATCGTCGTAT	LUCIFERASE	LUCIFERASE	CONTROL	CDS		n/a
49	TRCN0000231692	CAACGAGGACTACACCATCGT	RFP	RFP	CONTROL	CDS		n/a
50	TRCN0000231686	CAGTTCCAGTACGGCTCCAAG	RFP	RFP	CONTROL	CDS		n/a
51	TRCN0000231694	CCACTACGACGCCGAGGTCAA	RFP	RFP	CONTROL	CDS		n/a
52	TRCN0000231688	CCTGCAGGACGGCGAGTTCAT	RFP	RFP	CONTROL	CDS		n/a
53	TRCN0000231698	CGAGTTCGAGATCGAGGGCGA	RFP	RFP	CONTROL	CDS		n/a
54	TRCN0000231703	GCTCCGTGAACGGCCACGAGT	RFP	RFP	CONTROL	CDS		n/a
55	TRCN0000231684	GTACGGCTCCAAGGCCTACGT	RFP	RFP	CONTROL	CDS		n/a
56	TRCN0000231690	CGTAATGCAGAAGAAGACCAT	RFP	RFP	CONTROL	CDS		n/a
57	TRCN0000231689	CTACAAGACCGACATCAAGCT	RFP	RFP	CONTROL	CDS		n/a
58	TRCN0000231723	CTACACCATCGTGGAACAGTA	RFP	RFP	CONTROL	CDS		n/a
59	TRCN0000231683	CTCAGTTCCAGTACGGCTCCA	RFP	RFP	CONTROL	CDS		n/a
60	TRCN0000231691	GAACGGCCACGAGTTCGAGAT	RFP	RFP	CONTROL	CDS		n/a
61	TRCN0000231725	ACTACACCATCGTGGAACAGT	RFP	RFP	CONTROL	CDS		n/a
62	TRCN0000231724	CCGTAATGCAGAAGAAGACCA	RFP	RFP	CONTROL	CDS		n/a
63	TRCN0000231682	GCTTCAAGTGGGAGCGCGTGA	RFP	RFP	CONTROL	CDS		n/a
64	TRCN0000231696	GTAATGCAGAAGAAGACCATG	RFP	RFP	CONTROL	CDS		n/a
65	TRCN0000231701	TCAGTTCCAGTACGGCTCCAA	RFP	RFP	CONTROL	CDS		n/a
66	TRCN0000231687	TGCAGAAGAAGACCATGGGCT	RFP	RFP	CONTROL	CDS		n/a
67	TRCN0000231711	CCCGTCAGTATCGGCGGAATT	lacZ	lacZ	CONTROL	CDS		n/a
68	TRCN0000231738	CCGTCATAGCGATAACGAGTT	lacZ	lacZ	CONTROL	CDS		n/a
69	TRCN0000231722	CGCGATCGTAATCACCCGAGT	lacZ	lacZ	CONTROL	CDS		n/a
70	TRCN0000231743	CGCGCCTTTCGGCGGTGAAAT	lacZ	lacZ	CONTROL	CDS		n/a
71	TRCN0000231700	GCCGTCGTATTACAACGTCGT	lacZ	lacZ	CONTROL	CDS		n/a
72	TRCN0000231706	GTCGGCTTACGGCGGTGATTT	lacZ	lacZ	CONTROL	CDS		n/a
73	TRCN0000231712	GCGATCGTAATCACCCGAGTG	lacZ	lacZ	CONTROL	CDS		n/a
74	TRCN0000231704	GCGTTGGCAATTTAACCGCCA	lacZ	lacZ	CONTROL	CDS		n/a
75	TRCN0000231717	CGTCGTATTACAACGTCGTGA	lacZ	lacZ	CONTROL	CDS		n/a
76	TRCN0000231702	TCGTATTACAACGTCGTGACT	lacZ	lacZ	CONTROL	CDS		n/a
77	TRCN0000231726	TGTTCGCATTATCCGAACCAT	lacZ	lacZ	CONTROL	CDS		n/a
78	TRCN0000231716	CGACCACGCAAATCAGCGATT	lacZ	lacZ	CONTROL	CDS		n/a
79	TRCN0000231708	GCGCTAATCACGACGCGCTGT	lacZ	lacZ	CONTROL	CDS		n/a
80	TRCN0000231685	GTTCCGTCATAGCGATAACGA	lacZ	lacZ	CONTROL	CDS		n/a
81	TRCN0000231710	CGCTAAATACTGGCAGGCGTT	lacZ	lacZ	CONTROL	CDS		n/a
82	TRCN0000231735	CCAACGTGACCTATCCCATTA	lacZ	lacZ	CONTROL	CDS		n/a
83	TRCN0000231713	ACTCTGGCTAACGGTACGCGT	lacZ	lacZ	CONTROL	CDS		n/a
84	TRCN0000231709	CGATCGTAATCACCCGAGTGT	lacZ	lacZ	CONTROL	CDS		n/a
85	TRCN0000241923		(no hairpin)	n/a	n/a	n/a		n/a