Construct: ORF TRCN0000470877
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011040.1_s317c1
- Derived from:
- BRDN0000395429
- DNA Barcode:
- GCCCTTTAGGCACCTCGCCTTCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNNM3 (26505)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470877
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26505 | CNNM3 | cyclin and CBS domain dival... | NM_017623.5 | 99.9% | 100% | 894C>T |
| 2 | human | 26505 | CNNM3 | cyclin and CBS domain dival... | XM_011510957.3 | 97.9% | 96.6% | (many diffs) |
| 3 | human | 26505 | CNNM3 | cyclin and CBS domain dival... | XM_017003800.2 | 97.6% | 97% | (many diffs) |
| 4 | human | 26505 | CNNM3 | cyclin and CBS domain dival... | NM_199078.3 | 93.1% | 93.2% | 894C>T;1225_1226ins144 |
| 5 | human | 26505 | CNNM3 | cyclin and CBS domain dival... | XR_244887.5 | 43.8% | (many diffs) | |
| 6 | human | 26505 | CNNM3 | cyclin and CBS domain dival... | XR_001738700.2 | 40.8% | (many diffs) | |
| 7 | human | 26505 | CNNM3 | cyclin and CBS domain dival... | XR_001738701.2 | 40.6% | (many diffs) | |
| 8 | human | 26505 | CNNM3 | cyclin and CBS domain dival... | XR_001738702.2 | 32.9% | (many diffs) | |
| 9 | mouse | 94218 | Cnnm3 | cyclin M3 | NM_053186.3 | 85.1% | 86.2% | (many diffs) |
| 10 | mouse | 94218 | Cnnm3 | cyclin M3 | NM_001331209.1 | 85% | 86% | (many diffs) |
| 11 | mouse | 94218 | Cnnm3 | cyclin M3 | NM_001039551.2 | 83.4% | 84.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2187
- ORF length:
- 2121
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggcggta gctgcggcgg gtcggttagg ctggttgttc gccgcgctct 121 gcctgggcaa cgccgcgggg gaggccgcgc cgggcccgcg agtgctgggc ttctgcctgg 181 aggaggatgg agcggcgggc gcgggttggg tacgcggagg ggcggcgcgg gacacgccgg 241 acgccacctt cctcctgcgc ctcttcggcc cgggcttcgc caacagctct tggtcctggg 301 tggccccgga gggggcgggc tgccgggagg aggcggcctc ccccgcgggc gagtggcgcg 361 cgctgctgcg cttgcgcctg cgggccgagg ccgtgcgccc gcactcggcg ctgctggcgg 421 tgcgcgtgga gccgggtggc ggggcggctg aggaggcggc gccgccctgg gctctgggcc 481 tgggggcggc cgggctgctg gcgctggcag cgctggcgcg aggcctgcag ctgagcgcgc 541 tggcgctggc gcctgccgag gtgcaggtgc tgcgcgagag cggctcggag gcggagcgtg 601 cggcggcgcg gcgtttggag cccgcgcggc gctgggccgg ctgcgccttg ggcgcgctgc 661 tgctgctggc cagcctggcg caggcggcgc tggcggtgct gctgtaccgc gcggccggcc 721 agcgtgcggt gcccgccgtg ttgggcagcg cggggctcgt gttcctggtg ggagaggtgg 781 tgccggccgc cgtgagcggg cgctggacgc tggcgctggc gccgcgagcg ctcggcctca 841 gccgcctggc cgtcctgctc actctgcccg tcgcgctgcc cgtggggcag ctgctggagc 901 tggcggcgcg gcccgggcgg ctgcgggagc gggtgctgga gctggcgcgc ggcggcggtg 961 acccctacag cgatctcagc aagggcgtgc tgcgctgccg gaccgtggag gacgtgctca 1021 cgcccctcga agactgcttc atgctggacg ccagcaccgt gctggacttc ggcgtcctgg 1081 ccagcatcat gcagagcggc cacacgcgca tcccggtgta cgaggaggag cgctccaaca 1141 tcgtggacat gctctacctc aaggacttgg ccttcgtgga tcccgaagac tgcacgccgc 1201 tcagcaccat cactcgtttc tacaaccatc cgctccactt cgtcttcaac gacaccaagc 1261 tggacgctgt cctggaggaa ttcaagcgag ggaagtccca cctggccatc gtgcagaagg 1321 tgaacaacga gggtgaaggc gaccccttct acgaggtcct gggcctggtc accctggagg 1381 acgtgatcga ggagatcatc aggtccgaga tcctggacga gtctgaagac taccgagaca 1441 ccgtggtgaa gaggaagcct gcttctctga tggcccctct gaagcggaag gaggagttct 1501 ccttgttcaa ggtgtctgat gatgaatata aagtaacaat ctcgcctcag ctgctcttgg 1561 ccacccagcg cttcctgtcc cgagaagtgg atgtattcag cccgctgcgc atctctgaga 1621 aggtcctgct gcacctgttg aagcatccca gtgtcaacca ggaagtgagg tttgacgaga 1681 gcaaccggct ggccacacac cactacctgt accagcgcag ccagccggtg gattacttca 1741 ttctcaTCCT GCAGGGCAGG GTTGAAGTGG AGATCGGGAA AGAGGGTCTG AAGTTTGAGA 1801 ATGGGGCCTT CACGTACTAT GGAGTGTCGG CCCTAACTGT GCCATCCTCG GTTCACCAGT 1861 CCCCGGTGTC CTCGCTCCAG CCCATCCGCC ATGACCTGCA GCCCGACCCA GGTGACGGCA 1921 CGCATTCATC TGCGTATTGT CCCGACTACA CCGTGAGGGC GCTCTCTGAT CTGCAGCTCA 1981 TCAAGGTTAC GCGACTGCAG TACCTCAATG CACTCCTGGC TACCCGAGCC CAGAACCTGC 2041 CACAGTCCCC TGAGAACACC GACCTGCAGG TTATTCCAGG CAGCCAGACC AGGCTCCTTG 2101 GTGAGAAGAC CACCACAGCG GCAGGGTCCA GCCACAGCAG GCCCGGCGTC CCGGTGGAAG 2161 GCAGCCCTGG GCGGAACCCA GGCGTTTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 2221 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2281 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCCCTTTA 2341 GGCACCTCGC CTTCATACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2401 aagatt