Construct: ORF TRCN0000471890
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009486.1_s317c1
- Derived from:
- ccsbBroadEn_05195
- DNA Barcode:
- TCAGTCACTACGGACACAAGATGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARL13B (200894)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471890
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 200894 | ARL13B | ADP ribosylation factor lik... | NM_001174150.2 | 100% | 100% | |
| 2 | human | 200894 | ARL13B | ADP ribosylation factor lik... | NM_182896.3 | 100% | 100% | |
| 3 | human | 200894 | ARL13B | ADP ribosylation factor lik... | XM_011512535.2 | 94.1% | 94.1% | 0_1ins75 |
| 4 | human | 200894 | ARL13B | ADP ribosylation factor lik... | NM_001321328.2 | 92.1% | 91.2% | (many diffs) |
| 5 | human | 200894 | ARL13B | ADP ribosylation factor lik... | XM_006713532.3 | 92.1% | 91.2% | (many diffs) |
| 6 | human | 200894 | ARL13B | ADP ribosylation factor lik... | XM_011512534.2 | 92.1% | 91.2% | (many diffs) |
| 7 | human | 200894 | ARL13B | ADP ribosylation factor lik... | XM_011512532.2 | 89% | 88.6% | 0_1ins91;39_93del |
| 8 | human | 200894 | ARL13B | ADP ribosylation factor lik... | XM_011512533.2 | 89% | 88.6% | 0_1ins91;39_93del |
| 9 | human | 200894 | ARL13B | ADP ribosylation factor lik... | NM_001174151.1 | 75.9% | 75.9% | 0_1ins309 |
| 10 | human | 200894 | ARL13B | ADP ribosylation factor lik... | XM_017005853.1 | 75.9% | 75.9% | 0_1ins309 |
| 11 | human | 200894 | ARL13B | ADP ribosylation factor lik... | NM_144996.4 | 75% | 75% | 59_60ins321 |
| 12 | human | 200894 | ARL13B | ADP ribosylation factor lik... | NR_033427.2 | 26% | (many diffs) | |
| 13 | human | 200894 | ARL13B | ADP ribosylation factor lik... | NR_135621.2 | 25.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1353
- ORF length:
- 1284
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttcagtctg atggccagtt gctgcggctg gttcaagcgg tggcgggagc 121 ctgtcagaaa ggtgactctt ttgatggtgg gacttgataa tgctggtaaa accgcaacag 181 caaagggaat ccaaggagaa taccctgaag atgtagctcc tactgttgga ttttcaaaaa 241 ttaaccttag acaaggaaag tttgaagtca ccatctttga cttgggaggt ggaataagaa 301 ttcggggaat ctggaagaat tactatgctg aatcctatgg ggtaatattt gttgtggatt 361 ccagtgatga agagagaatg gaagagacaa aagaggctat gtcagaaatg ctaagacatc 421 ctaggatatc gggaaagcct atattggtgt tggcaaataa acaagataaa gaaggagctt 481 taggagaagc tgatgtcatt gaatgtctat ctctggaaaa attggtcaat gagcacaagt 541 gcctgtgtca gatagaacca tgttcagcaa tctcggggta tggaaagaaa attgacaagt 601 ccattaaaaa aggcctttat tggctgctac atgttattgc aagagacttt gatgccttaa 661 atgaacgcat ccaaaaagag acaacagagc agcgtgctct tgaggaacaa gagaaacaag 721 aaagagctga acgagtgcga aaattacgag aagaaagaaa acaaaatgaa caggagcagg 781 ctgaactcga tggaaccagt ggtctggctg agttggaccc agaaccaacg aatcctttcc 841 agccaatagc atctgtaatc attgagaatg aaggaaaact tgaaagagag aaaaaaaacc 901 aaaaaatgga gaaagacagt gatggctgcc acctgaaaca taaaaTGGAG CATGAGCAAA 961 TAGAGACACA AGGCCAGGTT AATCACAATG GCCAAAAAAA TAATGAATTT GGACTAGTAG 1021 AAAATTATAA GGAGGCATTA ACACAGCAGT TAAAGAATGA AGATGAGACA GACCGGCCAT 1081 CATTGGAATC AGCTAATGGT AAAAAGAAAA CTAAGAAACT AAGAATGAAA AGGAACCACC 1141 GGGTAGAACC ACTTAATATA GATGACTGTG CTCCTGAGAG TCCAACGCCA CCCCCACCCC 1201 CTCCTCCTGT TGGCTGGGGA ACCCCTAAAG TCACTAGACT TCCAAAACTT GAGCCTCTTG 1261 GTGAAACACA TCATAATGAT TTCTATAGGA AGCCACTGCC TCCCCTGGCT GTGCCACAGC 1321 GACCTAACAG TGATGCTCAT GATGTGATCT CATTGCCAAC TTTCTTGTAC AAAGTGGTTG 1381 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1441 GTCCGTAACT TGAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCA 1501 GTCACTACGG ACACAAGATG AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt 1561 tgtgaaagat t