Construct: ORF TRCN0000475165

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006620.1_s317c1
Derived from:
ccsbBroadEn_15990
DNA Barcode:
TATAAAGATTGACATCCGACGTGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACBD4 (79777)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475165
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79777 ACBD4 acyl-CoA binding domain con... NM_001135705.3 99.7% 100% 906C>T;915G>A
2 human 79777 ACBD4 acyl-CoA binding domain con... NM_024722.4 99.7% 100% 906C>T;915G>A
3 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025091.1 99.7% 100% 906C>T;915G>A
4 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025092.2 99.7% 100% 906C>T;915G>A
5 human 79777 ACBD4 acyl-CoA binding domain con... XM_006722085.2 99.4% 99.6% 573_575delGCA;909C>T;918G>A
6 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025090.1 97.5% 97.7% 573_593del;927C>T;936G>A
7 human 79777 ACBD4 acyl-CoA binding domain con... NM_001135706.3 89.2% 55.7% 500_537del;944C>T;953_1023delinsA
8 human 79777 ACBD4 acyl-CoA binding domain con... NM_001321352.2 89.2% 55.7% 500_537del;944C>T;953_1023delinsA
9 human 79777 ACBD4 acyl-CoA binding domain con... NM_001321353.2 89.2% 55.7% 500_537del;944C>T;953_1023delinsA
10 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025088.1 89.2% 55.7% 500_537del;944C>T;953_1023delinsA
11 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025087.1 88.9% 55.7% (many diffs)
12 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025084.1 87.4% 56.9% (many diffs)
13 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025085.1 87.4% 56.9% (many diffs)
14 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025086.1 87.4% 56.9% (many diffs)
15 human 79777 ACBD4 acyl-CoA binding domain con... NM_001135707.3 87.1% 65.2% (many diffs)
16 human 79777 ACBD4 acyl-CoA binding domain con... XM_024450949.1 86.7% 80.4% (many diffs)
17 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025089.2 86.4% 80.1% (many diffs)
18 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025094.1 84.9% 55.1% (many diffs)
19 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025093.1 82.8% 56.7% (many diffs)
20 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025099.1 81.6% 67.7% 647_648ins140;766C>T;775_807delinsA
21 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025097.1 81.1% 72.4% (many diffs)
22 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025098.1 81.1% 72.4% (many diffs)
23 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025096.1 79.8% 66.3% (many diffs)
24 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025095.1 79.3% 71.3% (many diffs)
25 human 79777 ACBD4 acyl-CoA binding domain con... XM_011525259.2 73.7% 53.1% (many diffs)
26 human 79777 ACBD4 acyl-CoA binding domain con... XM_011525261.2 72.5% 71.8% (many diffs)
27 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025100.1 72.1% 54.3% (many diffs)
28 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025101.2 70.9% 70.2% (many diffs)
29 human 79777 ACBD4 acyl-CoA binding domain con... XM_024450950.1 60.4% 32.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
981
ORF length:
915
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg caccgagaaa gaaagcccag agcccgactg ccagaaacag ttccaggctg
121cagtgagcgt catccagaac ctgcccaaga acggttctta ccgcccctcc tatgaagaga
181tgctgcgatt ctacagttac tacaagcagg ccaccatggg gccctgcctg gtcccccggc
241ccgggttctg ggaccccatt ggacgatata agtgggacgc ctggaacagt ctgggcaaga
301tgagcaggga ggaggccatg tctgcctaca tcactgaaat gaaactggtg gcacagaagg
361tgatcgacac agtgcccctg ggtgaggtgg cagaggacat gtttggttac ttcgagcccc
421tgtaccaggt gatccctgac atgccgaggc ccccagagac cttcctgaga agggtcacag
481gttggaaaga gcaggttgtg aatggagatg ttggggctgt ttcagagcct ccctgcctcc
541ccaaggaacc ggcaccccca agcccagagt cccattcacc cagggacctg gactccgagg
601ttttctgtga ttccctggag cagctggagc ctgagctggt ttggacagag cagcgggcag
661catctggagg aaagcgtgat cccaggaaca gccccgtgcc ccccacaaag aaagaggggt
721tgcggggcag cccgccgggg ccccaggagt tggacgtgtg gctgctgggg acagttcgag
781cactacagga gagcatgcag gaggtgcagg cgagggtgca gagcctggag agcatgcccc
841ggccccctga gcagaggccg cagcccaggc ccagtgctcg gccatggccc cttgggctcc
901cggggcccgc gctgctcttc ttcctcctgt ggcccTTCGT CGTCCAGTGG CTCTTCCGAA
961TGTTTCGGAC TCAAAAGAGA TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1021CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1081AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATATA AAGATTGACA
1141TCCGACGTGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)