Construct: ORF TRCN0000487784

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020655.1_s317c1
DNA Barcode:
CACAGGGCATCCGCATTTATCAGC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
MKNK1 (8569)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487784
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711000.1 94.3% 79.7% 1005_1048del;1245_1272del
2 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711001.3 92.6% 77.1% (many diffs)
3 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711002.3 92.6% 77.1% (many diffs)
4 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711003.1 92.6% 77.1% (many diffs)
5 human 8569 MKNK1 MAPK interacting serine/thr... NM_001135553.4 91.5% 76.8% 0_1ins36;969_1012del;1209_1236del
6 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450508.1 91.5% 76.8% 0_1ins36;969_1012del;1209_1236del
7 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450509.1 91.5% 76.8% 0_1ins36;969_1012del;1209_1236del
8 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450510.1 91.5% 76.8% 0_1ins36;969_1012del;1209_1236del
9 human 8569 MKNK1 MAPK interacting serine/thr... XM_017002659.1 86.7% 100% 1041_1042ins159
10 human 8569 MKNK1 MAPK interacting serine/thr... XM_017002660.2 85% 96.8% (many diffs)
11 human 8569 MKNK1 MAPK interacting serine/thr... XM_017002661.2 85% 96.8% (many diffs)
12 human 8569 MKNK1 MAPK interacting serine/thr... XM_017002662.1 85% 96.8% (many diffs)
13 human 8569 MKNK1 MAPK interacting serine/thr... NM_198973.5 83.7% 96.5% 0_1ins36;1005_1006ins159
14 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450512.1 83.7% 96.5% 0_1ins36;1005_1006ins159
15 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450513.1 83.7% 96.5% 0_1ins36;1005_1006ins159
16 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450514.1 83.7% 96.5% 0_1ins36;1005_1006ins159
17 human 8569 MKNK1 MAPK interacting serine/thr... NM_003684.7 83.4% 70.1% (many diffs)
18 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711007.1 81.3% 62.7% (many diffs)
19 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450511.1 81.3% 62.7% (many diffs)
20 human 8569 MKNK1 MAPK interacting serine/thr... XM_017002652.2 79.4% 61.6% (many diffs)
21 human 8569 MKNK1 MAPK interacting serine/thr... XM_017002653.1 79.4% 61.6% (many diffs)
22 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711013.1 71.9% 51.4% (many diffs)
23 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711014.1 71.9% 51.4% (many diffs)
24 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711015.1 71.9% 51.4% (many diffs)
25 human 8569 MKNK1 MAPK interacting serine/thr... NR_146512.2 46% 1_176del;1015_1045del;1408_2606del
26 human 8569 MKNK1 MAPK interacting serine/thr... NR_024174.3 43% 1_218del;532_533ins74;1345_2543del
27 human 8569 MKNK1 MAPK interacting serine/thr... NR_024176.3 40.7% (many diffs)
28 mouse 17346 Mknk1 MAP kinase-interacting seri... NM_001285487.1 81.4% 72.5% (many diffs)
29 mouse 17346 Mknk1 MAP kinase-interacting seri... XM_006502822.3 81.4% 72.5% (many diffs)
30 mouse 17346 Mknk1 MAP kinase-interacting seri... XM_006502823.3 81.1% 72.8% (many diffs)
31 mouse 17346 Mknk1 MAP kinase-interacting seri... NM_001285488.1 80.2% 72.3% (many diffs)
32 mouse 17346 Mknk1 MAP kinase-interacting seri... NM_021461.5 80.2% 72.3% (many diffs)
33 mouse 17346 Mknk1 MAP kinase-interacting seri... XM_006502825.3 75.7% 90.7% (many diffs)
34 mouse 17346 Mknk1 MAP kinase-interacting seri... XM_017320015.1 74.5% 90.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
99
ORF end:
1140
ORF length:
1041
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggctccgc ggccgccccc ttcaccggat ccttacagat ggtatcttct caaaagttgg
121aaaaacctat agagatgggc agtagcgaac cccttcccat cgcagatggt gacaggagga
181ggaagaagaa gcggaggggc cgggccactg actccttgcc aggaaagttt gaagatatgt
241acaagctgac ctctgaattg cttggagagg gagcctatgc caaagttcaa ggtgccgtga
301gcctacagaa tggcaaagag tatgccgtca aaatcatcga gaaacaagca gggcacagtc
361ggagtagggt gtttcgagag gtggagacgc tgtatcagtg tcagggaaac aagaacattt
421tggagctgat tgagttcttt gaagatgaca caaggtttta cttggtcttt gagaaattgc
481aaggaggttc catcttagcc cacatccaga agcaaaagca cttcaatgag cgagaagcca
541gccgagtggt gcgggacgtt gctgctgccc ttgacttcct gcataccaaa ggcattgctc
601atcgtgatct gaaaccagaa aatatattgt gtgaatctcc agaaaaggtg tctccagtga
661aaatctgtga ctttgacttg ggcagtggga tgaaactgaa caactcctgt acccccataa
721ccacaccaga gctgaccacc ccatgtggct ctgcagaata catggcccct gaggtagtgg
781aggtcttcac ggaccaggcc acattctacg acaagcgctg tgacctgtgg agcctgggcg
841tggtcctcta catcatgctg agtggctacc cacccttcgt gggtcactgc ggggccgact
901gtggctggga ccggggcgag gtctgcaggg tgtgccagaa caagctgttt gaaagcatcc
961aggaaggcaa gtatgagttt cctgacaagg actgggcaca catctccagt gaagccaaag
1021acctcatctc caagctccTG GTGCGAGATG CAAAGCAGAG ACTTAGCGCC GCCCAAGTTC
1081TGCAGCACCC ATGGGTGCAG GGGGAACAGC AGCACAATGG ACCTGACGCT CTTCGCAGCT
1141GAGGCCATCG CCCTTAACCG CCAGCTATCT CAGCACGAAG AGAACGAACT AGCAGAGGAG
1201CCAGAGGCAC TAGCTGATGG CCTCTGCTCC ATGAAGCTTT CCCCTCCCTG CAAGTCACGC
1261CTGGCCCGGA GACGGGCCCT GGCCCAGGCA GGCCGTGGTG AAGACAGGAG CCCGCCCACA
1321GCACTCAAGG GTGGGCGCGC CGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG
1381CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT
1441GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACAC AGGGCATCCG
1501CATTTATCAG CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat
1561t

Download FASTA (ORF) (Full)