Construct: ORF TRCN0000489474
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020089.1_s317c1
- DNA Barcode:
- CATCAGAGCTTCTTGCTCATCAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IKBKB (3551)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489474
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3551 | IKBKB | inhibitor of nuclear factor... | NM_001556.3 | 100% | 100% | |
| 2 | human | 3551 | IKBKB | inhibitor of nuclear factor... | NM_001190720.2 | 95.2% | 93.9% | (many diffs) |
| 3 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_005273492.4 | 93.3% | 93.1% | 2114_2115insTGAAGAACTGGTGGCTGAA;2117C>A;2118_2119ins131 |
| 4 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_005273490.3 | 92.5% | 90% | (many diffs) |
| 5 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_011544517.2 | 92.5% | 90% | (many diffs) |
| 6 | human | 3551 | IKBKB | inhibitor of nuclear factor... | NM_001242778.2 | 92.1% | 91.2% | 0_1ins82;21_22ins95 |
| 7 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_005273494.3 | 88.8% | 87.2% | (many diffs) |
| 8 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_011544518.2 | 86.3% | 81.9% | (many diffs) |
| 9 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_005273491.5 | 85.2% | 82% | (many diffs) |
| 10 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_011544519.2 | 85.2% | 82% | (many diffs) |
| 11 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_011544520.2 | 79.9% | 72.7% | (many diffs) |
| 12 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_005273493.4 | 79.5% | 74.5% | (many diffs) |
| 13 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_005273495.2 | 66.5% | 61.3% | (many diffs) |
| 14 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_005273496.4 | 66.5% | 61.3% | (many diffs) |
| 15 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_005273498.4 | 66.5% | 61.3% | (many diffs) |
| 16 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_011544521.2 | 66.5% | 61.3% | (many diffs) |
| 17 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_011544522.2 | 58% | 55.9% | (many diffs) |
| 18 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_017013396.1 | 58% | 55.9% | (many diffs) |
| 19 | human | 3551 | IKBKB | inhibitor of nuclear factor... | NR_033818.2 | 56.7% | 1_186del;575_630del;2511_3994del | |
| 20 | human | 3551 | IKBKB | inhibitor of nuclear factor... | NR_040009.2 | 56.2% | 1_186del;1309_1310ins53;2402_3885del | |
| 21 | human | 3551 | IKBKB | inhibitor of nuclear factor... | NR_033819.2 | 56% | 1_186del;1702_1703ins62;2393_3876del | |
| 22 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XR_001745530.2 | 53.8% | (many diffs) | |
| 23 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XR_949402.3 | 46.8% | (many diffs) | |
| 24 | mouse | 16150 | Ikbkb | inhibitor of kappaB kinase ... | NM_001159774.1 | 87% | 92.4% | (many diffs) |
| 25 | mouse | 16150 | Ikbkb | inhibitor of kappaB kinase ... | XM_006509019.3 | 85.9% | 90.6% | (many diffs) |
| 26 | mouse | 16150 | Ikbkb | inhibitor of kappaB kinase ... | NM_010546.2 | 84.9% | 90.7% | (many diffs) |
| 27 | mouse | 16150 | Ikbkb | inhibitor of kappaB kinase ... | XM_006509020.3 | 81.8% | 87.5% | (many diffs) |
| 28 | mouse | 16150 | Ikbkb | inhibitor of kappaB kinase ... | XM_006509021.2 | 63.2% | 64.2% | (many diffs) |
| 29 | mouse | 16150 | Ikbkb | inhibitor of kappaB kinase ... | XM_006509022.3 | 62% | 65.3% | (many diffs) |
| 30 | mouse | 16150 | Ikbkb | inhibitor of kappaB kinase ... | XM_006509023.3 | 48.5% | 51.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 99
- ORF end:
- 2367
- ORF length:
- 2268
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggccgccccc ttcacccaat tgatcagtat gagctggtca ccttccctga 121 caacgcagac atgtggggcc tgggaaatga aagagcgcct tgggacaggg ggatttggaa 181 atgtcatccg atggcacaat caggaaacag gtgagcagat tgccatcaag cagtgccggc 241 aggagctcag cccccggaac cgagagcggt ggtgcctgga gatccagatc atgagaaggc 301 tgacccaccc caatgtggtg gctgcccgag atgtccctga ggggatgcag aacttggcgc 361 ccaatgacct gcccctgctg gccatggagt actgccaagg aggagatctc cggaagtacc 421 tgaaccagtt tgagaactgc tgtggtctgc gggaaggtgc catcctcacc ttgctgagtg 481 acattgcctc tgcgcttaga taccttcatg aaaacagaat catccatcgg gatctaaagc 541 cagaaaacat cgtcctgcag caaggagaac agaggttaat acacaaaatt attgacctag 601 gatatgccaa ggagctggat cagggcagtc tttgcacatc attcgtgggg accctgcagt 661 acctggcccc agagctactg gagcagcaga agtacacagt gaccgtcgac tactggagct 721 tcggcaccct ggcctttgag tgcatcacgg gcttccggcc cttcctcccc aactggcagc 781 ccgtgcagtg gcattcaaaa gtgcggcaga agagtgaggt ggacattgtt gttagcgaag 841 acttgaatgg aacggtgaag ttttcaagct ctttacccta ccccaataat cttaacagtg 901 tcctggctga gcgactggag aagtggctgc aactgatgct gatgtggcac ccccgacaga 961 ggggcacgga tcccacgtat gggcccaatg gctgcttcaa ggccctggat gacatcttaa 1021 acttaaagct ggttcatatc ttgaacatgg tcacgggcac catccacacc taccctgtga 1081 cagaggatga gagtctgcag agcttgaagg ccagaatcca acaggacacg ggcatcccag 1141 aggaggacca ggagctgctg caggaagcgg gcctggcgtt gatccccgat aagcctgcca 1201 ctcagtgtat ttcagacggc aagttaaatg agggccacac attggacatg gatcttgttt 1261 ttctctttga caacagtaaa atcacctatg agactcagat ctccccacgg ccccaacctg 1321 aaagtgtcag ctgtatcctt caagagccca agaggaatct cgccttcttc cagctgagga 1381 aggtgtgggg ccaggtctgg cacagcatcc agaccctgaa ggaagattgc aaccggctgc 1441 agcagggaca gcgagccgcc atgatgaatc tcctccgaaa caacagctgc ctctccaaaa 1501 tgaagaattc catggcttcc atgtctcagc agctcaaggc caagttggat ttcttcaaaa 1561 ccagcatcca gattgacctg gagaagtaca gcgagcaaac cgagtttggg atcacatcag 1621 ataaactgct gctggcctgg agggaaatgg agcaggctgt ggagctctgt gggcgggaga 1681 acgaagtgaa actcctggta gaacggatga tggctctgca gaccgacatt gtggacttac 1741 agaggagccc catgggccgg aagcaggggg gaacgctgga cgacctagag gagcaagcaa 1801 gggagctgta caggagacta agggaaaaac ctcgagacca gcgaactgag ggtgacagtc 1861 aggaaatggt acggctgctg cttcaggcaa ttcagagctt cgagaagaaa gtgcgagtga 1921 tctatacgca gctcagtaaa actgtggttt gcaagcagaa ggcgctggaa ctgttgccca 1981 aGGTGGAAGA GGTGGTGAGC TTAATGAATG AGGATGAGAA GACTGTTGTC CGGCTGCAGG 2041 AGAAGCGGCA GAAGGAGCTC TGGAATCTCC TGAAGATTGC TTGTAGCAAG GTCCGTGGTC 2101 CTGTCAGTGG AAGCCCGGAT AGCATGAATG CCTCTCGACT TAGCCAGCCT GGGCAGCTGA 2161 TGTCTCAGCC CTCCACGGCC TCCAACAGCT TACCTGAGCC AGCCAAGAAG AGTGAAGAAC 2221 TGGTGGCTGA AGCACATAAC CTCTGCACCC TGCTAGAAAA TGCCATACAG GACACTGTGA 2281 GGGAACAAGA CCAGAGTTTC ACGGCCCTAG ACTGGAGCTG GTTACAGACG GAAGAAGAAG 2341 AGCACAGCTG CCTGGAGCAG GCCTCAAAGG GTGGGCGCGC CGACCCAGCT TTCTTGTACA 2401 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2461 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2521 AGGACGACAT CAGAGCTTCT TGCTCATCAG TACGCGTTAA GTCgacaatc aacctctgga 2581 ttacaaaatt tgtgaaagat t