Construct: ORF ccsbBroad304_02688
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011563.1_s304c1, BRDN0000400262
- Derived from:
- ccsbBroadEn_02688
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTF2 (22823)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02688
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22823 | MTF2 | metal response element bind... | NM_001164392.1 | 100% | 100% | |
| 2 | human | 22823 | MTF2 | metal response element bind... | NM_007358.4 | 90.3% | 90.3% | 990_1160del |
| 3 | human | 22823 | MTF2 | metal response element bind... | NM_001164391.1 | 73.1% | 73.1% | 0_1ins306;684_854del |
| 4 | human | 22823 | MTF2 | metal response element bind... | NM_001164393.1 | 73.1% | 73.1% | 0_1ins306;684_854del |
| 5 | mouse | 17765 | Mtf2 | metal response element bind... | NM_013827.3 | 86.1% | 88.1% | (many diffs) |
| 6 | mouse | 17765 | Mtf2 | metal response element bind... | NM_001359088.1 | 84.4% | 86.2% | (many diffs) |
| 7 | mouse | 17765 | Mtf2 | metal response element bind... | XM_011249412.3 | 84.4% | 86.2% | (many diffs) |
| 8 | mouse | 17765 | Mtf2 | metal response element bind... | XM_030254189.1 | 84.4% | 86.2% | (many diffs) |
| 9 | mouse | 17765 | Mtf2 | metal response element bind... | XM_030254190.1 | 81.7% | 83.8% | (many diffs) |
| 10 | mouse | 17765 | Mtf2 | metal response element bind... | NM_001253877.1 | 69.5% | 71.5% | (many diffs) |
| 11 | mouse | 17765 | Mtf2 | metal response element bind... | NM_001253878.1 | 69.5% | 71.5% | (many diffs) |
| 12 | mouse | 17765 | Mtf2 | metal response element bind... | NM_001253879.1 | 69.5% | 71.5% | (many diffs) |
| 13 | mouse | 17765 | Mtf2 | metal response element bind... | NM_001253880.1 | 69.5% | 71.5% | (many diffs) |
| 14 | mouse | 17765 | Mtf2 | metal response element bind... | NM_001359089.1 | 69.5% | 71.5% | (many diffs) |
| 15 | mouse | 17765 | Mtf2 | metal response element bind... | XM_011249416.3 | 69.5% | 71.5% | (many diffs) |
| 16 | mouse | 17765 | Mtf2 | metal response element bind... | XM_030254191.1 | 69.5% | 71.5% | (many diffs) |
| 17 | mouse | 17765 | Mtf2 | metal response element bind... | XM_030254192.1 | 69.5% | 71.5% | (many diffs) |
| 18 | mouse | 17765 | Mtf2 | metal response element bind... | XM_030254193.1 | 69.5% | 71.5% | (many diffs) |
| 19 | mouse | 17765 | Mtf2 | metal response element bind... | XM_030254194.1 | 69.5% | 71.5% | (many diffs) |
| 20 | mouse | 17765 | Mtf2 | metal response element bind... | XM_030254195.1 | 69.5% | 71.5% | (many diffs) |
| 21 | mouse | 17765 | Mtf2 | metal response element bind... | XR_389261.4 | 64.3% | (many diffs) | |
| 22 | mouse | 17765 | Mtf2 | metal response element bind... | XM_030254196.1 | 61.7% | 61.7% | (many diffs) |
| 23 | mouse | 17765 | Mtf2 | metal response element bind... | XM_011249417.3 | 60.4% | 60.1% | (many diffs) |
| 24 | mouse | 17765 | Mtf2 | metal response element bind... | NR_152817.1 | 32.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1674
- ORF length:
- 1608
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag agactctaca ggggcaggta attcactggt ccacaagcgg tctcctttac 121 gtcgaaacca aaagacccca acatccttga ccaagctgtc tttacaggat ggacataaag 181 ccaaaaagcc agcatgtaaa tttgaagagg gtcaggatgt cctagctaga tggtcagatg 241 gcttgtttta tcttggcact atcaaaaaga taaacatatt gaaacagagc tgcttcatca 301 tatttgaaga cagttctaaa tcctgggttc tctggaagga cattcaaaca ggagccactg 361 gaagtgggga aatggtctgt acaatatgtc aagaagagta ttcagaagct cccaatgaaa 421 tggttatatg tgacaagtgt ggccaaggat atcatcagtt gtgtcacaca cctcatattg 481 attccagtgt gattgattca gatgaaaaat ggctctgtcg gcagtgtgtt tttgcaacaa 541 caacaaagag gggtggtgca cttaagaaag gaccaaatgc caaagcattg caagtcatga 601 agcagacatt accctatagt gtggcagacc ttgaatggga tgcaggtcat aaaaccaatg 661 tccagcagtg ttactgctat tgtggaggcc ctggagactg gtatttgaag atgctacagt 721 gctgcaaatg taagcagtgg tttcatgagg cttgtgtgca atgccttcaa aagccaatgc 781 tatttggaga cagattttat acgtttatat gctctgtctg cagttctgga ccagaatacc 841 tcaaacgtct accattacag tgggtagata tagcacacct atgcctttac aacctaagtg 901 ttattcataa gaagaaatac tttgattctg aacttgagct tatgacatac attaatgaaa 961 actgggatag attgcaccct ggagagctgg cagacacacc aaaatctgaa agatatgagc 1021 atgttctgga ggcattaaat gattacaaga ccatggaagt aagcaatggc atagaaaaaa 1081 aaggaaagaa aaaatctgta ggtcgtccac ctggcccata tacaagaaaa atgattcaaa 1141 aaactgctga gccacttttg gataaggaat caatttcaga gaatcctact ttggatttac 1201 cttgttctat agggagaact gagggaactg cacattcatc caatacctca gatgtggatt 1261 tcacgggtgc ttccagtgca aaagaaacta cctcgtctag catttccagg cattatggat 1321 tatctgactc cagaaaaaga acgcgtacag gaagatcttg gcctgctgca ataccacatt 1381 tgcggagaag aagaggtcgt cttccaagaa gagcactcca gactcagaac tcagaaattg 1441 taaaagatga tgaaggcaaa gaagattatc agtttgatga actcaacaca gagattctga 1501 ataacttagc agatcaggag ttacaactca atcatctaaa gaactccatt accagttatt 1561 ttggtgctgc aggtagaata gcatgtggcg aaaaataccg agttttggca cgtcgggtga 1621 cacttgatgg aaaggtgcag tatcttgtgg aatgggaagg agcaactgca tcctgcccaa 1681 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1741 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta