Construct: ORF ccsbBroad304_08635

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001484.1_s304c1, BRDN0000408585
Derived from:
ccsbBroadEn_08635
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ZNF253 (56242)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08635
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 56242 ZNF253 zinc finger protein 253 NM_021047.3 99.8% 100% 1005A>T;1017C>T
2 human 56242 ZNF253 zinc finger protein 253 NM_001331133.1 87% 87.1% 0_1ins192;813A>T;825C>T
3 human 56242 ZNF253 zinc finger protein 253 NM_001331134.1 84.6% 84.7% 0_1ins228;777A>T;789C>T
4 human 199777 ZNF626 zinc finger protein 626 NM_001076675.3 82.2% 71% (many diffs)
5 human 100129842 ZNF737 zinc finger protein 737 NM_001159293.2 80.5% 69.7% (many diffs)
6 human 440515 ZNF506 zinc finger protein 506 NM_001099269.3 79.2% 69.6% (many diffs)
7 human 100129842 ZNF737 zinc finger protein 737 XM_006722597.4 78.5% 68% (many diffs)
8 human 100129842 ZNF737 zinc finger protein 737 XM_011527599.3 78.5% 68% (many diffs)
9 human 100129842 ZNF737 zinc finger protein 737 XM_005259697.4 78.4% 67.8% (many diffs)
10 human 100129842 ZNF737 zinc finger protein 737 XM_011527598.3 78.4% 67.8% (many diffs)
11 human 90649 ZNF486 zinc finger protein 486 NM_052852.4 77% 65.6% (many diffs)
12 human 100129842 ZNF737 zinc finger protein 737 XM_017026116.1 74.8% 63.9% (many diffs)
13 human 7617 ZNF66 zinc finger protein 66 NM_001355197.2 74.2% 64.2% (many diffs)
14 human 440515 ZNF506 zinc finger protein 506 NM_001145404.2 73.3% 63.8% (many diffs)
15 human 7639 ZNF85 zinc finger protein 85 NM_003429.5 72% 60.8% (many diffs)
16 human 81931 ZNF93 zinc finger protein 93 NM_031218.4 69.3% 58.7% (many diffs)
17 human 7643 ZNF90 zinc finger protein 90 NM_007138.2 69.1% 58% (many diffs)
18 human 7639 ZNF85 zinc finger protein 85 NM_001256171.1 68.6% 57.7% (many diffs)
19 human 440519 ZNF724 zinc finger protein 724 NM_001355404.2 68.2% 58.3% (many diffs)
20 human 100129842 ZNF737 zinc finger protein 737 XM_017026117.2 67.4% 56.7% (many diffs)
21 human 7639 ZNF85 zinc finger protein 85 XM_011528263.2 67% 56.5% (many diffs)
22 human 148213 ZNF681 zinc finger protein 681 NM_138286.3 65.7% 54.9% (many diffs)
23 human 7639 ZNF85 zinc finger protein 85 NM_001256173.1 62.6% 51.7% (many diffs)
24 human 100129842 ZNF737 zinc finger protein 737 XR_001753570.2 60.3% (many diffs)
25 human 7608 ZNF56 zinc finger protein 56 NM_001355194.2 41.6% 32.8% (many diffs)
26 human 105372319 LOC105372319 zinc finger protein 430-like XM_017027571.1 32.3% 21.6% (many diffs)
27 human 7643 ZNF90 zinc finger protein 90 XR_001753753.2 30.3% (many diffs)
28 human 440519 ZNF724 zinc finger protein 724 XM_024451521.1 13.6% 12.6% (many diffs)
29 human 51427 ZNF107 zinc finger protein 107 XM_024446791.1 13.2% 11.6% (many diffs)
30 human 7697 ZNF138 zinc finger protein 138 NM_001271637.2 10.9% 8.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1566
ORF length:
1497
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gggaccattg caatttagag atgtggccat agaattctct ctggaggagt
121ggcattgcct ggacactgca cagcggaatt tatataggga tgtgatgtta gagaactaca
181gaaacttggt cttccttggt attgttgtct ctaagccaga cctggttacc tgtctggagc
241aaggaaaaaa acctttaact atggaaagac atgagatgat tgccaaaccc ccagttatga
301gttctcattt tgcccaagac ctttggccag agaacataca aaattctttc caaataggga
361tgctgagaag atatgaagaa tgcagacatg acaatttaca gttaaaaaaa ggctgtaaaa
421gcgtgggtga gcataaggtg cacaaaggag gttataatgg acttaaccaa tgtttgacaa
481ctacccagaa agaaatattt caatgtgata aatatggaaa agtctttcat aagttttcaa
541attcaaacac atataagaca agacatactg gaataaatct tttcaaatgt ataatatgtg
601gcaaagcttt taaacggtcc tcaaccctta ctacacataa gaaaattcat actggagaga
661aaccttacag atgtgaagaa tgtggcaaag cttttaacca atctgcaaac cttactacac
721ataagagaat tcataccgga gagaaaccct acagatgtga agaatgtggc aaagccttta
781agcagtcctc aaaccttact acacataaga aaattcatac tggagagaaa ccctacaaat
841gtgaagaatg tggcaaagcc ttcaaccgat ccacagacct tactacacat aagatagttc
901atactggaga gaaaccctac aaatgtgaag aatgtggcaa agcctttaag cacccctcac
961acgttaccac acataagaaa attcatacta gagggaaacc ctacaactgt gaagaatgtg
1021gcaaatcctt taagcactgc tctaacctta ctatacataa gagaattcat actggagaga
1081aaccttacaa atgtgaagaa tgtggcaaag cctttcacct atcctcacac cttactacac
1141ataagatact tcatactgga gagaaaccct acagatgtag agaatgtggc aaagctttta
1201accattccac aacccttttt tcacatgaga aaattcatac tggagagaaa ccctacaaat
1261gtgatgaatg tggcaaaacc tttacctggc cctcaatcct ctccaaacat aaaagaactc
1321atactggaga gaaaccctac aaatgtgaag aatgtggcaa atcctttact gcatcctcaa
1381ctctaactac acataagaga attcatactg gagagaaacc ttacaaatgt gaagaatgtg
1441gcaaagcttt taactggtcc tcagacctta ataaacataa gaaaattcat attgaacgaa
1501aaccctacat agtgaagaat gtgacagatc ttttaaatgt tcctccactt ttaattagca
1561taagattgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
1621gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
1681acctctggat ta

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