Construct: ORF ccsbBroad304_14622

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019049.1_s304c1, BRDN0000459848
Derived from:
ccsbBroadEn_14622
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
EGFR (1956)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14622
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1956 EGFR epidermal growth factor rec... NM_005228.5 81.4% 26.7% (many diffs)
2 human 1956 EGFR epidermal growth factor rec... NM_005228.3:c.2239_2247del 81.2% 26.8% (many diffs)
3 human 1956 EGFR epidermal growth factor rec... NM_005228.3:c.2240_2254del 81.1% 26.8% (many diffs)
4 human 1956 EGFR epidermal growth factor rec... NM_001346899.1 77.9% 22.9% (many diffs)
5 human 1956 EGFR epidermal growth factor rec... NM_001346900.2 77.3% 22.3% (many diffs)
6 human 1956 EGFR epidermal growth factor rec... NM_001346898.2 74.5% 28.5% (many diffs)
7 human 1956 EGFR epidermal growth factor rec... NM_001346897.2 71% 24.4% (many diffs)
8 human 1956 EGFR epidermal growth factor rec... NM_001346941.2 65% 12% (many diffs)
9 human 1956 EGFR epidermal growth factor rec... NM_201284.2 49% 45.9% (many diffs)
10 human 1956 EGFR epidermal growth factor rec... NM_201282.2 44.6% 51.5% (many diffs)
11 human 1956 EGFR epidermal growth factor rec... NM_201283.1 31.5% 80% (many diffs)
12 mouse 13649 Egfr epidermal growth factor rec... NM_207655.2 72.6% 23.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1056
ORF length:
987
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gcgaccctcc gggacggccg gggcagcgct cctggcgctg ctggctgcgc
121tctgcccggc gagtcgggct ctggaggaaa agaaagtttg ccaaggcacg agtaacaagc
181tcacgcagtt gggcactttt gaagatcatt ttctcagcct ccagaggatg ttcaataact
241gtgaggtggt ccttgggaat ttggaaatta cctatgtgca gaggaattat gatctttcct
301tcttaaagac catccaggag gtggctggtt atgtcctcat tgccctcaac acagtggagc
361gaattccttt ggaaaacctg cagatcatca gaggaaatat gtactacgaa aattcctatg
421ccttagcagt cttatctaac tatgatgcaa ataaaaccgg actgaaggag ctgcccatga
481gaaatttaca ggaaatcctg catggcgccg tgcggttcag caacaaccct gccctgtgca
541acgtggagag catccagtgg cgggacatag tcagcagtga ctttctcagc aacatgtcga
601tggacttcca gaaccacctg ggcagctgcc aaaagtgtga tccaagctgt cccaatggga
661gctgctgggg tgcaggagag gagaactgcc agaaactgac caaaatcatc tgtgcccagc
721agtgctccgg gcgctgccgt ggcaagtccc ccagtgactg ctgccacaac cagtgtgctg
781caggctgcac aggcccccgg gagagcgact gcctggtctg ccgcaaattc cgagacgaag
841ccacgtgcaa ggacacctgc cccccactca tgctctacaa ccccaccacg taccagatgg
901atgtgaaccc cgagggcaaa tacagctttg gtgccacctg cgtgaagaag tgtccccgta
961attatgtggt gacagatcac ggctcgtgcg tccgagcctg tggggccgac agctatgaga
1021tggaggaaga cggcgtccgc aCTAGTACGA CGTTATAAGG TCGCCCGGGG GCAGTGTATA
1081CGACCATTGG GACGAATCTG TTGTAAATGG TTTCAAAATA GCTGCTCTAA TATGGAAATT
1141AAGAGGCCGG GGGGCCTCGG TCTAAGTATA ATAAAAACAT GATCCGCCGT ACAGCGGAGA
1201AAGGGGGGGA CTGGAGGAGT TAGATGGCGG GTAAGGGAGT CGGGGCGGGA TAGCTAGAGC
1261CAGACAGATA AGATACATTG AGAGTTTGGA CAATCCACAA CTAGAATGCC GTGAAAAAAA
1321TGATTTATGT GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT
1381AAACAAGTTC CTCTCACTCT GTGATATTCA TTTCTTTGCA AGTTATAAAT AGTGAATAAT
1441AAGATGACAT GAACTACTAT ACGTACTGCT AGAGATTTTC CAGACTGACT AAAAGGGTCT
1501GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTA GAAGCACTCA
1561AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC
1621TCAGATCTGG TCTAACCAGA GAGACCCGTT TATGTATCGA GCTAGGCACT TAAATACAAT
1681ATCTTTGCAA TGCGGCAATT CAGTGGTTCG TCCAATCCAT GTCAGACCCG TCTGTTGCCT
1741TCCTAATAAG GCACGATCGT ACCACCTGAC TTCCACCAAT CGGCATGCAC GGTGCTTTTT
1801CTCTCCTTGT AAGGCAAGTT GCTAACTCAT CGTTACCATG TTGCAAGACT ACAAGTTTAT
1861TGCATAAGAC TACATTAAGC TTGCAGCTCC AGCTTTTGTT CCCGTTAGTG AGGGTAAATG
1921CGCGCTGGCG TTTCCGGCCT Attcatgcga aggcgccaca tcgttcggaa gcgcacgctg
1981cggaggctgc tgcaggagag ggagcttgtg gagcctctta cacccagtgg agaagctccc
2041aaccaagctc tcttgaggat cttgaaggaa actgaattca aaaagatcaa agtgctgggc
2101tccggtgcgt tcggcacggt gtataaggga ctctggatcc cagaaggtga gaaagttaaa
2161attcccgtcg ctatcaagga attaagagaa gcaacatctc cgaaagccaa caaggaaatc
2221ctcgatgaag cctacgtgat ggccagcgtg gacaaccccc acgtgtgccg cctgctgggc
2281atctgcctca cctccaccgt gcagctcatc acgcagctca tgcccttcgg ctgcctcctg
2341gactatgtcc gggaacacaa agacaatatt ggctcccagt acctgctcaa ctggtgtgtg
2401cagatcgcaa agggcatgaa ctacttggag gaccgtcgct tggtgcaccg cgacctggca
2461gccaggaacg tactggtgaa aacaccgcag catgtcaaga tcacagattt tgggctggcc
2521aaactgctgg gtgcggaaga gaaagaatac catgcagaag gaggcaaagt gcctatcaag
2581tggatggcat tggaatcaat tttacacaga atctataccc accagagtga tgtctggagc
2641tacggggtga ccgtttggga gttgatgacc tttggatcca agccatatga cggaatccct
2701gccagcgaga tctcctccat cctggagaaa ggagaacgcc tccctcagcc acccatatgt
2761accatcgatg tctacatgat catggtcaag tgctggatga tagacgcaga tagtcgccca
2821aagttccgtg agttgatcat cgaattctcc aaaatggccc gagaccccca gcgctacctt
2881gtcattcagg gggatgaaag aatgcatttg ccaagtccta cagactccaa cttctaccgt
2941gccctgatgg atgaagaaga catggacgac gtggtggatg ccgacgagta cctcatccca
3001cagcagggct tcttcagcag cccctccacg tcacggactc ccctcctgag ctctctgagt
3061gcaaccagca acaattccac cgtggcttgc attgatagaa atgggctgca aagctgtccc
3121atcaaggaag acagcttctt gcagcgatac agctcagacc ccacaggcgc cttgactgag
3181gacagcatag acgacacctt cctcccagtg cctgaataca taaaccagtc cgttcccaaa
3241aggcccgctg gctctgtgca gaatcctgtc tatcacaatc agcctctgaa ccccgcgccc
3301agcagagacc cacactacca ggacccccac agcactgcag tgggcaaccc cgagtatctc
3361aacactgtcc agcccacctg tgtcaacagc acattcgaca gccctgccca ctgggcccag
3421aaaggcagcc accaaattag cctggacaac cctgactacc agcaggactt ctttcccaag
3481gaagccaagc caaatggcat ctttaagggc tccacagctg aaaatgcaga atacctaagg
3541gtcgcgccac aaagcagtga atttattgga gcattgccaa ctttcttgta caaagtggtt
3601ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct
3661aaccggtggc gcgttaagtc gacaatcaac ctctggatta

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