Construct: ORF ccsbBroad304_14622
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019049.1_s304c1, BRDN0000459848
- Derived from:
- ccsbBroadEn_14622
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- EGFR (1956)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14622
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.5 | 81.4% | 26.7% | (many diffs) |
| 2 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2239_2247del | 81.2% | 26.8% | (many diffs) |
| 3 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2240_2254del | 81.1% | 26.8% | (many diffs) |
| 4 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346899.1 | 77.9% | 22.9% | (many diffs) |
| 5 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346900.2 | 77.3% | 22.3% | (many diffs) |
| 6 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346898.2 | 74.5% | 28.5% | (many diffs) |
| 7 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346897.2 | 71% | 24.4% | (many diffs) |
| 8 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346941.2 | 65% | 12% | (many diffs) |
| 9 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201284.2 | 49% | 45.9% | (many diffs) |
| 10 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201282.2 | 44.6% | 51.5% | (many diffs) |
| 11 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201283.1 | 31.5% | 80% | (many diffs) |
| 12 | mouse | 13649 | Egfr | epidermal growth factor rec... | NM_207655.2 | 72.6% | 23.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1056
- ORF length:
- 987
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcgaccctcc gggacggccg gggcagcgct cctggcgctg ctggctgcgc 121 tctgcccggc gagtcgggct ctggaggaaa agaaagtttg ccaaggcacg agtaacaagc 181 tcacgcagtt gggcactttt gaagatcatt ttctcagcct ccagaggatg ttcaataact 241 gtgaggtggt ccttgggaat ttggaaatta cctatgtgca gaggaattat gatctttcct 301 tcttaaagac catccaggag gtggctggtt atgtcctcat tgccctcaac acagtggagc 361 gaattccttt ggaaaacctg cagatcatca gaggaaatat gtactacgaa aattcctatg 421 ccttagcagt cttatctaac tatgatgcaa ataaaaccgg actgaaggag ctgcccatga 481 gaaatttaca ggaaatcctg catggcgccg tgcggttcag caacaaccct gccctgtgca 541 acgtggagag catccagtgg cgggacatag tcagcagtga ctttctcagc aacatgtcga 601 tggacttcca gaaccacctg ggcagctgcc aaaagtgtga tccaagctgt cccaatggga 661 gctgctgggg tgcaggagag gagaactgcc agaaactgac caaaatcatc tgtgcccagc 721 agtgctccgg gcgctgccgt ggcaagtccc ccagtgactg ctgccacaac cagtgtgctg 781 caggctgcac aggcccccgg gagagcgact gcctggtctg ccgcaaattc cgagacgaag 841 ccacgtgcaa ggacacctgc cccccactca tgctctacaa ccccaccacg taccagatgg 901 atgtgaaccc cgagggcaaa tacagctttg gtgccacctg cgtgaagaag tgtccccgta 961 attatgtggt gacagatcac ggctcgtgcg tccgagcctg tggggccgac agctatgaga 1021 tggaggaaga cggcgtccgc aCTAGTACGA CGTTATAAGG TCGCCCGGGG GCAGTGTATA 1081 CGACCATTGG GACGAATCTG TTGTAAATGG TTTCAAAATA GCTGCTCTAA TATGGAAATT 1141 AAGAGGCCGG GGGGCCTCGG TCTAAGTATA ATAAAAACAT GATCCGCCGT ACAGCGGAGA 1201 AAGGGGGGGA CTGGAGGAGT TAGATGGCGG GTAAGGGAGT CGGGGCGGGA TAGCTAGAGC 1261 CAGACAGATA AGATACATTG AGAGTTTGGA CAATCCACAA CTAGAATGCC GTGAAAAAAA 1321 TGATTTATGT GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT 1381 AAACAAGTTC CTCTCACTCT GTGATATTCA TTTCTTTGCA AGTTATAAAT AGTGAATAAT 1441 AAGATGACAT GAACTACTAT ACGTACTGCT AGAGATTTTC CAGACTGACT AAAAGGGTCT 1501 GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTA GAAGCACTCA 1561 AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC 1621 TCAGATCTGG TCTAACCAGA GAGACCCGTT TATGTATCGA GCTAGGCACT TAAATACAAT 1681 ATCTTTGCAA TGCGGCAATT CAGTGGTTCG TCCAATCCAT GTCAGACCCG TCTGTTGCCT 1741 TCCTAATAAG GCACGATCGT ACCACCTGAC TTCCACCAAT CGGCATGCAC GGTGCTTTTT 1801 CTCTCCTTGT AAGGCAAGTT GCTAACTCAT CGTTACCATG TTGCAAGACT ACAAGTTTAT 1861 TGCATAAGAC TACATTAAGC TTGCAGCTCC AGCTTTTGTT CCCGTTAGTG AGGGTAAATG 1921 CGCGCTGGCG TTTCCGGCCT Attcatgcga aggcgccaca tcgttcggaa gcgcacgctg 1981 cggaggctgc tgcaggagag ggagcttgtg gagcctctta cacccagtgg agaagctccc 2041 aaccaagctc tcttgaggat cttgaaggaa actgaattca aaaagatcaa agtgctgggc 2101 tccggtgcgt tcggcacggt gtataaggga ctctggatcc cagaaggtga gaaagttaaa 2161 attcccgtcg ctatcaagga attaagagaa gcaacatctc cgaaagccaa caaggaaatc 2221 ctcgatgaag cctacgtgat ggccagcgtg gacaaccccc acgtgtgccg cctgctgggc 2281 atctgcctca cctccaccgt gcagctcatc acgcagctca tgcccttcgg ctgcctcctg 2341 gactatgtcc gggaacacaa agacaatatt ggctcccagt acctgctcaa ctggtgtgtg 2401 cagatcgcaa agggcatgaa ctacttggag gaccgtcgct tggtgcaccg cgacctggca 2461 gccaggaacg tactggtgaa aacaccgcag catgtcaaga tcacagattt tgggctggcc 2521 aaactgctgg gtgcggaaga gaaagaatac catgcagaag gaggcaaagt gcctatcaag 2581 tggatggcat tggaatcaat tttacacaga atctataccc accagagtga tgtctggagc 2641 tacggggtga ccgtttggga gttgatgacc tttggatcca agccatatga cggaatccct 2701 gccagcgaga tctcctccat cctggagaaa ggagaacgcc tccctcagcc acccatatgt 2761 accatcgatg tctacatgat catggtcaag tgctggatga tagacgcaga tagtcgccca 2821 aagttccgtg agttgatcat cgaattctcc aaaatggccc gagaccccca gcgctacctt 2881 gtcattcagg gggatgaaag aatgcatttg ccaagtccta cagactccaa cttctaccgt 2941 gccctgatgg atgaagaaga catggacgac gtggtggatg ccgacgagta cctcatccca 3001 cagcagggct tcttcagcag cccctccacg tcacggactc ccctcctgag ctctctgagt 3061 gcaaccagca acaattccac cgtggcttgc attgatagaa atgggctgca aagctgtccc 3121 atcaaggaag acagcttctt gcagcgatac agctcagacc ccacaggcgc cttgactgag 3181 gacagcatag acgacacctt cctcccagtg cctgaataca taaaccagtc cgttcccaaa 3241 aggcccgctg gctctgtgca gaatcctgtc tatcacaatc agcctctgaa ccccgcgccc 3301 agcagagacc cacactacca ggacccccac agcactgcag tgggcaaccc cgagtatctc 3361 aacactgtcc agcccacctg tgtcaacagc acattcgaca gccctgccca ctgggcccag 3421 aaaggcagcc accaaattag cctggacaac cctgactacc agcaggactt ctttcccaag 3481 gaagccaagc caaatggcat ctttaagggc tccacagctg aaaatgcaga atacctaagg 3541 gtcgcgccac aaagcagtga atttattgga gcattgccaa ctttcttgta caaagtggtt 3601 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct 3661 aaccggtggc gcgttaagtc gacaatcaac ctctggatta