Transcript: Human NM_177534.2

Homo sapiens sulfotransferase family 1A member 1 (SULT1A1), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-09-24
Taxon:
Homo sapiens (human)
Gene:
SULT1A1 (6817)
Length:
1609
CDS:
474..1361

Additional Resources:

NCBI RefSeq record:
NM_177534.2
NBCI Gene record:
SULT1A1 (6817)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_177534.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000432180 ACCAAGCGGCTCAAGAATAAA pLKO_005 1409 3UTR 100% 15.000 10.500 N SULT1A1 n/a
2 TRCN0000035234 GAGAAGTTCATGGTCGGAGAA pLKO.1 951 CDS 100% 4.050 2.835 N SULT1A1 n/a
3 TRCN0000035236 ACGCAAAGGATGTGGCAGTTT pLKO.1 865 CDS 100% 4.950 2.970 N SULT1A1 n/a
4 TRCN0000035238 AGTTTCCTACTACCACTTCTA pLKO.1 881 CDS 100% 4.950 2.970 N SULT1A1 n/a
5 TRCN0000035235 GACATGAAGGAGAACCCGAAA pLKO.1 1056 CDS 100% 4.050 2.430 N SULT1A1 n/a
6 TRCN0000415811 GCTGAGGTGGGAGGATCATTT pLKO_005 1486 3UTR 100% 13.200 6.600 Y SULT1A1 n/a
7 TRCN0000254869 AGAAGGTCAAGGTGGTCTATG pLKO_005 835 CDS 100% 10.800 5.400 Y SULT1A4 n/a
8 TRCN0000434454 AGACCAACCTGGGCAACATAG pLKO_005 1522 3UTR 100% 10.800 5.400 Y SULT1A1 n/a
9 TRCN0000254871 TCCCGCTCATCAAGTACTTTG pLKO_005 526 CDS 100% 10.800 5.400 Y SULT1A4 n/a
10 TRCN0000419471 TGCCCTTCCTTGAGTTCAAAG pLKO_005 709 CDS 100% 10.800 5.400 Y SULT1A1 n/a
11 TRCN0000035202 CCTGTTCTCTACCTCTTCTAT pLKO.1 1032 CDS 100% 5.625 2.813 Y SULT1A2 n/a
12 TRCN0000035201 GTCCCGCTCATCAAGTACTTT pLKO.1 525 CDS 100% 5.625 2.813 Y SULT1A2 n/a
13 TRCN0000035200 CAGAAGGTCAAGGTGGTCTAT pLKO.1 834 CDS 100% 4.950 2.475 Y SULT1A2 n/a
14 TRCN0000181155 CAGAAGGTCAAGGTGGTCTAT pLKO.1 834 CDS 100% 4.950 2.475 Y SULT1A4 n/a
15 TRCN0000431947 CAGATTCTGGACATGATCTAC pLKO_005 639 CDS 100% 4.950 2.475 Y SULT1A2 n/a
16 TRCN0000035313 CCTCTTCTATGAAGACATGAA pLKO.1 1043 CDS 100% 4.950 2.475 Y SULT1A3 n/a
17 TRCN0000432229 GGTTCAGCACACGTCGTTCAA pLKO_005 1142 CDS 100% 4.950 2.475 Y SULT1A3 n/a
18 TRCN0000416831 AGCGCTTCGATGCGGACTATG pLKO_005 1294 CDS 100% 3.600 1.800 Y SULT1A2 n/a
19 TRCN0000035203 AGGAGATGAAGAAGAACCCTA pLKO.1 1162 CDS 100% 2.640 1.320 Y SULT1A2 n/a
20 TRCN0000035237 GATTCTGGACATGATCTACCA pLKO.1 641 CDS 100% 2.640 1.320 Y SULT1A1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_177534.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07015 pDONR223 100% 99.8% 99.6% None 638G>A n/a
2 ccsbBroad304_07015 pLX_304 0% 99.8% 99.6% V5 638G>A n/a
3 TRCN0000466799 CATACAATGTCGAAGTCAAGGAAT pLX_317 35.9% 99.8% 99.6% V5 638G>A n/a
4 ccsbBroadEn_01615 pDONR223 100% 96.9% 95.9% None (many diffs) n/a
5 ccsbBroad304_01615 pLX_304 0% 96.9% 95.9% V5 (many diffs) n/a
6 ccsbBroadEn_01621 pDONR223 100% 94.4% 92.8% None (many diffs) n/a
7 ccsbBroad304_01621 pLX_304 0% 94.4% 92.8% V5 (many diffs) n/a
8 TRCN0000467038 ATGCTACGCCCGAGCGTATCCGTA pLX_317 35.5% 94.4% 92.8% V5 (many diffs) n/a
9 ccsbBroadEn_07016 pDONR223 100% 94.2% 92.5% None (many diffs) n/a
10 ccsbBroad304_07016 pLX_304 0% 94.2% 92.5% V5 (many diffs) n/a
11 TRCN0000466576 ATCCCCCCGAGGACTACGCACATA pLX_317 30.4% 94.2% 92.5% V5 (many diffs) n/a
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