Construct: ORF TRCN0000466576

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008771.1_s317c1
Derived from:
ccsbBroadEn_07016
DNA Barcode:
ATCCCCCCGAGGACTACGCACATA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SULT1A3 (6818)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466576
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 445329 SULT1A4 sulfotransferase family 1A ... NM_001017390.2 99.7% 99.6% 4G>A;252C>T
2 human 6818 SULT1A3 sulfotransferase family 1A ... NM_177552.3 99.7% 99.6% 4G>A;252C>T
3 human 6817 SULT1A1 sulfotransferase family 1A ... NM_001055.3 94.2% 92.5% (many diffs)
4 human 6817 SULT1A1 sulfotransferase family 1A ... NM_177529.2 94.2% 92.5% (many diffs)
5 human 6817 SULT1A1 sulfotransferase family 1A ... NM_177530.2 94.2% 92.5% (many diffs)
6 human 6817 SULT1A1 sulfotransferase family 1A ... NM_177534.2 94.2% 92.5% (many diffs)
7 human 6817 SULT1A1 sulfotransferase family 1A ... XM_017023611.2 94.2% 92.5% (many diffs)
8 human 6817 SULT1A1 sulfotransferase family 1A ... XM_017023612.2 94.2% 92.5% (many diffs)
9 human 6817 SULT1A1 sulfotransferase family 1A ... XM_017023613.2 94.2% 92.5% (many diffs)
10 human 6817 SULT1A1 sulfotransferase family 1A ... XM_024450409.1 94.2% 92.5% (many diffs)
11 human 6817 SULT1A1 sulfotransferase family 1A ... XM_024450410.1 94.2% 92.5% (many diffs)
12 human 6817 SULT1A1 sulfotransferase family 1A ... XM_024450411.1 94.2% 92.5% (many diffs)
13 human 6799 SULT1A2 sulfotransferase family 1A ... NM_177528.2 93.3% 90.1% (many diffs)
14 human 6799 SULT1A2 sulfotransferase family 1A ... NM_001054.4 93.2% 89.8% (many diffs)
15 human 6817 SULT1A1 sulfotransferase family 1A ... XM_017023604.1 92.3% 90.6% (many diffs)
16 human 6817 SULT1A1 sulfotransferase family 1A ... XM_017023605.1 92.3% 90.6% (many diffs)
17 human 6817 SULT1A1 sulfotransferase family 1A ... XM_017023608.1 92.3% 90.6% (many diffs)
18 human 6817 SULT1A1 sulfotransferase family 1A ... XM_017023609.1 92.3% 90.6% (many diffs)
19 human 6817 SULT1A1 sulfotransferase family 1A ... XM_017023610.1 92.3% 90.6% (many diffs)
20 human 6817 SULT1A1 sulfotransferase family 1A ... XM_017023607.2 72% 70.7% (many diffs)
21 human 6817 SULT1A1 sulfotransferase family 1A ... XM_024450408.1 71.8% 70.5% (many diffs)
22 human 6799 SULT1A2 sulfotransferase family 1A ... NM_001363863.1 67.6% 61.9% (many diffs)
23 human 6817 SULT1A1 sulfotransferase family 1A ... NM_177536.3 64.6% 50.7% (many diffs)
24 human 6817 SULT1A1 sulfotransferase family 1A ... XR_001751973.1 61.4% (many diffs)
25 human 100526831 SLX1B-SULT1A4 SLX1B-SULT1A4 readthrough (... NR_037609.1 39.8% (many diffs)
26 human 100526830 SLX1A-SULT1A3 SLX1A-SULT1A3 readthrough (... NR_037608.1 39.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
951
ORF length:
885
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa gctgatccag gacacctccc gcccgccact ggagtacgtg aagggggtcc
121cgctcatcaa gtactttgca gaggcactgg ggcccctgca gagcttccaa gcccgacctg
181atgacctgct catcaacacc taccccaagt ctggcaccac ctgggtgagc cagatactgg
241acatgatcta ccagggcggc gacctagaga agtgtaaccg ggctcccatc tacgtacggg
301tgcccttcct tgaggttaat gatccagggg aaccctcagg gctggagact ctgaaagaca
361caccgccccc acggctcatc aagtcacacc tgcccctggc tctgctccct cagactctgt
421tggatcagaa ggtcaaggtg gtctatgttg cccgaaaccc aaaggacgtg gcggtctcct
481actaccattt ccaccgtatg gaaaaggcgc accctgagcc tgggacctgg gacagcttcc
541tggaaaagtt catggctgga gaagtgtcct acgggtcctg gtaccagcac gtgcaggagt
601ggtgggagct gagccgcacc caccctgttc tctacctctt ctatgaagac atgaaggaga
661accccaaaag ggagattcaa aagatCCTGG AGTTTGTGGG GCGCTCCCTG CCAGAGGAGA
721CCATGGACTT CATGGTTCAG CACACGTCGT TCAAGGAGAT GAAGAAGAAC CCTATGACCA
781ACTACACCAC CGTCCCCCAG GAGCTCATGG ACCACAGCAT CTCCCCCTTC ATGAGGAAAG
841GCATGGCTGG GGACTGGAAG ACCACCTTCA CCGTGGCGCA GAATGAGCGC TTCGATGCGG
901ACTATGCGGA GAAGATGGCA GGCTGCAGCC TCAGCTTCCG CTCTGAGCTG TGCCCAACTT
961TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1021CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1081CTTGTGGAAA GGACGAATCC CCCCGAGGAC TACGCACATA ACGCGTTAAG TCgacaatca
1141acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)