Transcript: Human NR_135758.2

Homo sapiens leucine rich repeat containing 28 (LRRC28), transcript variant 14, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
LRRC28 (123355)
Length:
5880
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_135758.2
NBCI Gene record:
LRRC28 (123355)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_135758.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000432959 ACCTCACTGTGGACCGAAATC pLKO_005 562 3UTR 100% 10.800 15.120 N LRRC28 n/a
2 TRCN0000005131 CGGTGTAGTGAGCCTATGTTT pLKO.1 1098 3UTR 100% 5.625 7.875 N LRRC28 n/a
3 TRCN0000005129 GCCTATGTTTACCATCGTCTA pLKO.1 1109 3UTR 100% 4.050 5.670 N LRRC28 n/a
4 TRCN0000423131 CCATCTTATCTGTACAATAAA pLKO_005 738 3UTR 100% 15.000 10.500 N LRRC28 n/a
5 TRCN0000434516 ACCAATCGTTTGCTAACTTTA pLKO_005 507 3UTR 100% 13.200 9.240 N LRRC28 n/a
6 TRCN0000425165 TTTGACCTGCTGAGTTGATAA pLKO_005 1236 3UTR 100% 13.200 9.240 N LRRC28 n/a
7 TRCN0000434786 ACTCCAATGTCTGGATCTTAG pLKO_005 347 3UTR 100% 10.800 7.560 N LRRC28 n/a
8 TRCN0000005130 GCTTTACGTCATCTTCGATTA pLKO.1 414 3UTR 100% 10.800 7.560 N LRRC28 n/a
9 TRCN0000010924 GATAACAACATTCACCTGAAA pLKO.1 711 3UTR 100% 4.950 3.465 N LRRC28 n/a
10 TRCN0000010923 CCAGTCCAGCACACTCTTCCA pLKO.1 1306 3UTR 100% 0.880 0.616 N LRRC28 n/a
11 TRCN0000256748 GGCAGGAGAATTGCTTGAATC pLKO_005 5172 3UTR 100% 10.800 5.400 Y SMIM11A n/a
12 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 2468 3UTR 100% 5.625 2.813 Y KLHL30 n/a
13 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 2468 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_135758.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04772 pDONR223 100% 17.9% None (many diffs) n/a
2 ccsbBroad304_04772 pLX_304 0% 17.9% V5 (many diffs) n/a
3 TRCN0000466194 TCCCGTCAACCGGTGAGCTGGTCA pLX_317 35.2% 17.9% V5 (many diffs) n/a
4 ccsbBroadEn_13098 pDONR223 100% 4.2% None (many diffs) n/a
5 ccsbBroad304_13098 pLX_304 0% 4.2% V5 (many diffs) n/a
6 TRCN0000468959 TCCTAGGGACCACCATATTTTTAG pLX_317 100% 4.2% V5 (many diffs) n/a
7 ccsbBroadEn_13766 pDONR223 100% 3.7% None (many diffs) n/a
8 ccsbBroad304_13766 pLX_304 0% 3.7% V5 (many diffs) n/a
9 TRCN0000474083 TCTCGACACAAACAGACTTCAACG pLX_317 100% 3.7% V5 (many diffs) n/a
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