Construct: ORF TRCN0000466709
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013520.1_s317c1
- Derived from:
- ccsbBroadEn_02416
- DNA Barcode:
- TCTCGCAGGACAGTTCGGCCAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUBB4B (10383)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466709
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10383 | TUBB4B | tubulin beta 4B class IVb | NM_006088.6 | 100% | 100% | |
| 2 | human | 347688 | TUBB8 | tubulin beta 8 class VIII | NM_177987.2 | 88.6% | 90.7% | (many diffs) |
| 3 | human | 260334 | TUBB8B | tubulin beta 8B | NM_001358689.1 | 88.2% | 88.9% | (many diffs) |
| 4 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_001289129.1 | 87.2% | 98.4% | (many diffs) |
| 5 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_006087.4 | 87.2% | 98.4% | (many diffs) |
| 6 | human | 7280 | TUBB2A | tubulin beta 2A class IIa | NM_001069.3 | 87.2% | 96.4% | (many diffs) |
| 7 | human | 347733 | TUBB2B | tubulin beta 2B class IIb | NM_178012.5 | 86.4% | 96.8% | (many diffs) |
| 8 | human | 203068 | TUBB | tubulin beta class I | NM_178014.4 | 84.4% | 97.3% | (many diffs) |
| 9 | human | 10381 | TUBB3 | tubulin beta 3 class III | NM_006086.4 | 84% | 91.7% | (many diffs) |
| 10 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303524.1 | 83.2% | 90.5% | (many diffs) |
| 11 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_032525.3 | 83.2% | 90.5% | (many diffs) |
| 12 | human | 203068 | TUBB | tubulin beta class I | NM_001293212.2 | 79.6% | 90.3% | (many diffs) |
| 13 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_001289127.1 | 79.2% | 89.3% | (many diffs) |
| 14 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_001289123.1 | 78.2% | 88.3% | (many diffs) |
| 15 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303526.2 | 77.4% | 83.1% | (many diffs) |
| 16 | human | 203068 | TUBB | tubulin beta class I | NM_001293214.2 | 75.5% | 87.8% | (many diffs) |
| 17 | human | 347688 | TUBB8 | tubulin beta 8 class VIII | XM_011519459.3 | 74.4% | 76.6% | (many diffs) |
| 18 | human | 260334 | TUBB8B | tubulin beta 8B | XM_024451145.1 | 74% | 75.5% | (many diffs) |
| 19 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_001289130.1 | 73.4% | 82.4% | (many diffs) |
| 20 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_001289131.1 | 73.4% | 82.4% | (many diffs) |
| 21 | human | 260334 | TUBB8B | tubulin beta 8B | XM_024451143.1 | 71.8% | 53.8% | (many diffs) |
| 22 | human | 260334 | TUBB8B | tubulin beta 8B | XM_024451144.1 | 71.8% | 53.8% | (many diffs) |
| 23 | human | 260334 | TUBB8B | tubulin beta 8B | XM_024451146.1 | 71.6% | 72.8% | (many diffs) |
| 24 | human | 10381 | TUBB3 | tubulin beta 3 class III | NM_001197181.2 | 71.4% | 77.7% | (many diffs) |
| 25 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303528.2 | 71.2% | 71.2% | (many diffs) |
| 26 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303527.2 | 70.7% | 76.9% | (many diffs) |
| 27 | human | 203068 | TUBB | tubulin beta class I | NM_001293215.2 | 70.5% | 82% | (many diffs) |
| 28 | human | 203068 | TUBB | tubulin beta class I | NM_001293216.2 | 70.5% | 82% | (many diffs) |
| 29 | human | 7280 | TUBB2A | tubulin beta 2A class IIa | NM_001310315.2 | 70.4% | 76.7% | (many diffs) |
| 30 | human | 347688 | TUBB8 | tubulin beta 8 class VIII | XM_017016192.2 | 66.3% | 68.3% | (many diffs) |
| 31 | human | 347688 | TUBB8 | tubulin beta 8 class VIII | XM_017016193.2 | 66.3% | 68.3% | (many diffs) |
| 32 | human | 260334 | TUBB8B | tubulin beta 8B | XM_024451147.1 | 65.4% | 67.4% | (many diffs) |
| 33 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303529.2 | 57.4% | 61.6% | (many diffs) |
| 34 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303530.2 | 57.4% | 61.6% | (many diffs) |
| 35 | human | 347688 | TUBB8 | tubulin beta 8 class VIII | XM_011519460.2 | 57% | 56.6% | (many diffs) |
| 36 | human | 260334 | TUBB8B | tubulin beta 8B | XM_024451148.1 | 56.8% | 55.9% | (many diffs) |
| 37 | human | 203068 | TUBB | tubulin beta class I | NM_001293213.2 | 46.5% | 52.1% | (many diffs) |
| 38 | human | 643224 | TUBBP5 | tubulin beta pseudogene 5 | NR_027156.1 | 43.7% | (many diffs) | |
| 39 | human | 203068 | TUBB | tubulin beta class I | NR_120608.2 | 24.9% | (many diffs) | |
| 40 | mouse | 227613 | Tubb4b | tubulin, beta 4B class IVB | NM_146116.2 | 90.1% | 100% | (many diffs) |
| 41 | mouse | 22151 | Tubb2a | tubulin, beta 2A class IIA | NM_009450.2 | 85.8% | 96.4% | (many diffs) |
| 42 | mouse | 73710 | Tubb2b | tubulin, beta 2B class IIB | NM_023716.2 | 85.4% | 96.8% | (many diffs) |
| 43 | mouse | 22154 | Tubb5 | tubulin, beta 5 class I | NM_011655.5 | 84.1% | 97.3% | (many diffs) |
| 44 | mouse | 22153 | Tubb4a | tubulin, beta 4A class IVA | NM_009451.3 | 83.9% | 98.4% | (many diffs) |
| 45 | mouse | 22152 | Tubb3 | tubulin, beta 3 class III | NM_023279.2 | 83.9% | 91.5% | (many diffs) |
| 46 | mouse | 67951 | Tubb6 | tubulin, beta 6 class V | NM_026473.2 | 82.2% | 90.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1401
- ORF length:
- 1335
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ggaaatcgtg cacttgcagg ccgggcagtg cggcaaccaa atcggcgcca 121 agttttggga ggtgatcagc gatgagcacg gcatcgaccc cacgggcacc taccacgggg 181 acagcgacct gcagctggaa cgcatcaacg tgtactacaa tgaggccacc ggcggcaagt 241 acgtgccccg cgccgtgctc gtggatctgg agcccggcac catggactcc gtgcgctcgg 301 ggcccttcgg gcagatcttc cggccggaca acttcgtttt cggtcagagt ggtgctggga 361 acaactgggc caaggggcac tacacagaag gcgcggagct ggtggactcg gtgctggatg 421 ttgtgagaaa ggaggctgag agctgtgact gcctgcaggg tttccagctg acccactccc 481 tgggtggggg gactgggtct gggatgggta ccctcctcat cagcaagatc cgggaggagt 541 acccagacag gatcatgaac acgtttagtg tggtgccttc gcccaaagtg tcagacacag 601 tggtggagcc ctacaacgcc accctctcag tccaccagct cgtagaaaac acagacgaga 661 cctactgcat tgataacgaa gctctctacg acatttgctt cagaacccta aagctgacca 721 cgcccaccta tggtgacctg aaccacctgg tgtctgctac catgagtggg gtcaccacct 781 gcctgcgctt cccaggccag ctcaatgctg acctgcggaa gctggctgtg aacatggtcc 841 cgtttccccg gctgcacttc ttcatgcccg gctttgcccc actgaccagc cggggcagcc 901 agcagtaccg ggcgctgacc gtgcccgagc tcacccagca gatgtttgat gccaagaaca 961 tgatggctgc cTGCGACCCC CGCCATGGCC GCTACCTGAC GGTTGCCGCC GTGTTCAGGG 1021 GCCGCATGTC CATGAAGGAG GTGGATGAGC AAATGCTTAA TGTCCAAAAC AAAAACAGCA 1081 GCTATTTTGT TGAGTGGATC CCCAACAATG TGAAAACGGC TGTCTGTGAC ATCCCACCTC 1141 GGGGGCTAAA AATGTCCGCC ACCTTCATTG GCAACAGCAC GGCCATCCAG GAGCTGTTCA 1201 AGCGCATCTC CGAGCAGTTC ACGGCCATGT TCCGGCGCAA GGCCTTCCTG CACTGGTACA 1261 CGGGCGAGGG CATGGACGAG ATGGAGTTCA CCGAGGCCGA GAGCAACATG AATGACCTGG 1321 TGTCCGAGTA CCAGCAGTAC CAGGATGCCA CAGCCGAGGA GGAGGGCGAG TTCGAGGAGG 1381 AGGCTGAGGA GGAGGTGGCC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1441 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1501 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCTC GCAGGACAGT 1561 TCGGCCAACT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt