Construct: ORF TRCN0000470280
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003468.1_s317c1
- Derived from:
- ccsbBroadEn_11047
- DNA Barcode:
- ATCGGTGCCCCACATGACGGCCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POU2F2 (5452)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470280
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | NM_001247994.2 | 100% | 100% | |
2 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | NM_002698.5 | 86.3% | 83.3% | 1145_1280del;1337_1389del |
3 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | NM_001207026.1 | 85.6% | 82.6% | 499_546del;1193_1328del;1385_1401del |
4 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | NM_001207025.3 | 83.5% | 80.5% | 499_546del;1193_1328del;1385_1437del |
5 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_011527042.3 | 82.4% | 79.5% | 185_250del;1211_1346del;1403_1455del |
6 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_011527041.3 | 79.8% | 77% | (many diffs) |
7 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_011527043.3 | 74.4% | 74.4% | 185_250del;369_665del;862_909del |
8 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_005259010.4 | 68.2% | 65.8% | 1145_1280del;1337_1758del |
9 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_017026896.2 | 66.6% | 64.3% | (many diffs) |
10 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_017026894.2 | 66.4% | 64.1% | 499_546del;1193_1328del;1385_1806del |
11 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_017026893.2 | 65.7% | 63.4% | 185_250del;1211_1346del;1403_1824del |
12 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_017026895.2 | 65.6% | 62.6% | (many diffs) |
13 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_011527040.3 | 64.1% | 61.8% | (many diffs) |
14 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_017026892.2 | 57% | 55% | (many diffs) |
15 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_024451547.1 | 56.6% | 54.5% | (many diffs) |
16 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_017026891.2 | 56.5% | 54.5% | (many diffs) |
17 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_024451546.1 | 55.3% | 53.3% | (many diffs) |
18 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_017026884.2 | 55.3% | 53.3% | (many diffs) |
19 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_024451545.1 | 54.8% | 52.8% | (many diffs) |
20 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_017026885.2 | 54.6% | 52.2% | (many diffs) |
21 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_017026886.2 | 53.6% | 51.7% | (many diffs) |
22 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_017026887.2 | 53.6% | 51.7% | (many diffs) |
23 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_017026888.2 | 53.6% | 51.7% | (many diffs) |
24 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_017026889.2 | 53.6% | 51.7% | (many diffs) |
25 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XM_017026890.2 | 53.6% | 51.7% | (many diffs) |
26 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XR_001753710.2 | 17.5% | 1_79del;1280_6848del | |
27 | human | 5452 | POU2F2 | POU class 2 homeobox 2 | XR_001753709.2 | 16.5% | (many diffs) | |
28 | mouse | 18987 | Pou2f2 | POU domain, class 2, transc... | NM_001163556.1 | 81.5% | 82% | (many diffs) |
29 | mouse | 18987 | Pou2f2 | POU domain, class 2, transc... | XM_006539662.3 | 80% | 79.8% | (many diffs) |
30 | mouse | 18987 | Pou2f2 | POU domain, class 2, transc... | NM_011138.2 | 78.8% | 79.3% | (many diffs) |
31 | mouse | 18987 | Pou2f2 | POU domain, class 2, transc... | NM_001163555.1 | 77.8% | 78.3% | (many diffs) |
32 | mouse | 18987 | Pou2f2 | POU domain, class 2, transc... | XM_006539661.2 | 77.3% | 77.1% | (many diffs) |
33 | mouse | 18987 | Pou2f2 | POU domain, class 2, transc... | XM_006539660.3 | 76.4% | 76.2% | (many diffs) |
34 | mouse | 18987 | Pou2f2 | POU domain, class 2, transc... | XM_006539659.2 | 68.7% | 69.2% | (many diffs) |
35 | mouse | 18987 | Pou2f2 | POU domain, class 2, transc... | XM_006539658.3 | 67.5% | 67.3% | (many diffs) |
36 | mouse | 18987 | Pou2f2 | POU domain, class 2, transc... | NM_001163554.1 | 64.7% | 65.1% | (many diffs) |
37 | mouse | 18987 | Pou2f2 | POU domain, class 2, transc... | XM_006539656.3 | 63.6% | 63.4% | (many diffs) |
38 | mouse | 18987 | Pou2f2 | POU domain, class 2, transc... | XM_006539655.3 | 62.5% | 62.3% | (many diffs) |
39 | mouse | 18987 | Pou2f2 | POU domain, class 2, transc... | XM_006539654.3 | 61.9% | 61.7% | (many diffs) |
40 | mouse | 18987 | Pou2f2 | POU domain, class 2, transc... | XM_006539653.3 | 59.8% | 59.6% | (many diffs) |
41 | mouse | 18987 | Pou2f2 | POU domain, class 2, transc... | XM_006539652.3 | 53.7% | 53.5% | (many diffs) |
42 | mouse | 18987 | Pou2f2 | POU domain, class 2, transc... | XM_006539651.3 | 53.2% | 53% | (many diffs) |
43 | mouse | 18987 | Pou2f2 | POU domain, class 2, transc... | XM_006539648.2 | 52.8% | 53.2% | (many diffs) |
44 | mouse | 18987 | Pou2f2 | POU domain, class 2, transc... | XM_006539647.3 | 52% | 51.8% | (many diffs) |
45 | mouse | 18987 | Pou2f2 | POU domain, class 2, transc... | XM_006539649.3 | 51.2% | 51.5% | (many diffs) |
46 | mouse | 18987 | Pou2f2 | POU domain, class 2, transc... | XM_006539650.3 | 51.2% | 51.5% | (many diffs) |
47 | mouse | 18987 | Pou2f2 | POU domain, class 2, transc... | XM_011250457.2 | 51.2% | 51.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1266
- ORF length:
- 1200
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt tcactccagc atgggggctc cagaaataag aatgtctaag cccctggagg 121 ccgagaagca aggtctggac tccccatcag agcacacaga caccgaaaga aatggaccag 181 acactaatca tcagaacccc caaaataaga cctccccatt ctccgtgtcc ccaactggcc 241 ccagtacaaa gatcaaggct gaagacccca gtggcgattc agccccagca gcacccctgc 301 cccctcagcc ggcccagcct catctgcccc aggcccaact catgttgacg ggcagccagc 361 tagctgggga catacagcag ctcctccagc tccagcagct ggtgcttgtg ccaggccacc 421 acctccagcc acctgctcag ttcctgctac cgcaggccca gcagagccag ccaggcctgc 481 taccgacacc aaatctattc cagctacctc agcaaaccca gggagctctt ctgacctccc 541 agccccgggc cgggcttccc acacagcccc ccaaatgctt ggagccacca tcccaccccg 601 aggagcccag tgatctggag gagctggagc aattcgcccg caccttcaag caacgccgca 661 tcaagctggg cttcacgcag ggtgatgtgg gcctggccat gggcaagctc tacggcaacg 721 acttcagcca gacgaccatt tcccgcttcg aggccctcaa cctgagcttc aagaacatgt 781 gcaaactcaa gcccctcctg gagaagtggc tcaacgatgc agagactatg tctgtggact 841 caagcctgcc cagccccaac cagctgagca gccccagcct gggtttcgac ggcctgcccg 901 gccggagacg caagaagagg accagcatcg agacaaacgt ccgcttcgcc ttagagaaga 961 gttttctagc gaaccagaag cctaccTCAG AGGAGATCCT GCTGATCGCC GAGCAGCTGC 1021 ACATGGAGAA GGAAGTGATC CGCGTCTGGT TCTGCAACCG GCGCCAGAAG GAGAAACGCA 1081 TCAACCCCTG CAGTGCGGCC CCCATGCTGC CCAGCCCAGG GAAGCCGGCC AGCTACAGCC 1141 CCCATATGGT CACACCCCAA GGGGGCGCGG GGACCTTACC GTTGTCCCAA GCTTCCAGCA 1201 GTCTGAGCAC AACAGCACAA ACCCCAGCCC TCAAGGCAGC CACTCGGCTA TCGGCTTGTC 1261 AGGCCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1321 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1381 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATCGGTGCC CCACATGACG GCCCAACGCG 1441 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt