Construct: ORF TRCN0000480554
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013971.1_s317c1
- Derived from:
- ccsbBroadEn_06871
- DNA Barcode:
- CGCCACACCACTTTAATCCAGGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNH1 (6050)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480554
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6050 | RNH1 | ribonuclease/angiogenin inh... | NM_002939.4 | 99.7% | 99.7% | 563G>A;570G>A;1239C>G |
| 2 | human | 6050 | RNH1 | ribonuclease/angiogenin inh... | NM_203383.2 | 99.7% | 99.7% | 563G>A;570G>A;1239C>G |
| 3 | human | 6050 | RNH1 | ribonuclease/angiogenin inh... | NM_203384.2 | 99.7% | 99.7% | 563G>A;570G>A;1239C>G |
| 4 | human | 6050 | RNH1 | ribonuclease/angiogenin inh... | NM_203385.1 | 99.7% | 99.7% | 563G>A;570G>A;1239C>G |
| 5 | human | 6050 | RNH1 | ribonuclease/angiogenin inh... | NM_203386.3 | 99.7% | 99.7% | 563G>A;570G>A;1239C>G |
| 6 | human | 6050 | RNH1 | ribonuclease/angiogenin inh... | NM_203387.3 | 99.7% | 99.7% | 563G>A;570G>A;1239C>G |
| 7 | human | 6050 | RNH1 | ribonuclease/angiogenin inh... | NM_203388.3 | 99.7% | 99.7% | 563G>A;570G>A;1239C>G |
| 8 | human | 6050 | RNH1 | ribonuclease/angiogenin inh... | NM_203389.3 | 99.7% | 99.7% | 563G>A;570G>A;1239C>G |
| 9 | human | 6050 | RNH1 | ribonuclease/angiogenin inh... | XM_011520255.1 | 99.7% | 99.7% | 563G>A;570G>A;1239C>G |
| 10 | human | 6050 | RNH1 | ribonuclease/angiogenin inh... | XM_011520257.3 | 99.7% | 99.7% | 563G>A;570G>A;1239C>G |
| 11 | human | 6050 | RNH1 | ribonuclease/angiogenin inh... | XM_011520258.3 | 99.7% | 99.7% | 563G>A;570G>A;1239C>G |
| 12 | human | 6050 | RNH1 | ribonuclease/angiogenin inh... | XM_011520259.3 | 99.7% | 99.7% | 563G>A;570G>A;1239C>G |
| 13 | human | 6050 | RNH1 | ribonuclease/angiogenin inh... | XM_011520260.2 | 99.7% | 99.7% | 563G>A;570G>A;1239C>G |
| 14 | human | 6050 | RNH1 | ribonuclease/angiogenin inh... | XM_011520261.2 | 99.7% | 99.7% | 563G>A;570G>A;1239C>G |
| 15 | human | 6050 | RNH1 | ribonuclease/angiogenin inh... | XM_011520262.3 | 99.7% | 99.7% | 563G>A;570G>A;1239C>G |
| 16 | human | 6050 | RNH1 | ribonuclease/angiogenin inh... | XM_011520263.1 | 99.7% | 99.7% | 563G>A;570G>A;1239C>G |
| 17 | human | 6050 | RNH1 | ribonuclease/angiogenin inh... | XM_017018106.1 | 99.7% | 99.7% | 563G>A;570G>A;1239C>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1449
- ORF length:
- 1383
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cctggacatc cagagcctgg acatccagtg tgaggagctg agcgacgcta 121 gatgggccga gctcctccct ctgctccagc agtgccaagt ggtcaggctg gacgactgtg 181 gcctcacgga agcacggtgc aaggacatca gctctgcact tcgagtcaac cctgcactgg 241 cagagctcaa cctgcgcagc aacgagctgg gcgatgtcgg cgtgcattgc gtgctccagg 301 gcctgcagac cccctcctgc aagatccaga agctgagcct ccagaactgc tgcctgacgg 361 gggccggctg cggggtcctg tccagcacac tacgcaccct gcccaccctg caggagctgc 421 acctcagcga caacctcttg ggggatgcgg gcctgcagct gctctgcgaa ggactcctgg 481 acccccagtg ccgcctggaa aagctgcagc tggagtattg cagcctctcg gctgccagct 541 gcgagcccct ggcctccgtg ctcagggcca agccggactt caaggagctc acggttagca 601 acaacgacat caatgaggct ggcgtccatg tgctatgcca gggcctgaag gactccccct 661 gccagctgga ggcgctcaag ctggagagct gcggtgtgac atcagacaac tgccgggacc 721 tgtgcggcat tgtggcctcc aaggcctcgc tgcgggagct ggccctgggc agcaacaagc 781 tgggtgatgt gggcatggcg gagctgtgcc cagggctgct ccaccccagc tccaggctca 841 ggaccctgtg gatctgggag tgtggcatca ctgccaaggg ctgcggggat ctgtgccgtg 901 tcctcagggc caaggagagc ctgaaggagc tcagcctggc cggcaacgag ctgggggatg 961 agggtgcccg actgctgtgt gagaccctgc tggaacctgg ctgccagctG GAGTCGCTGT 1021 GGGTGAAGTC CTGCAGCTTC ACAGCCGCCT GCTGCTCCCA CTTCAGCTCA GTGCTGGCCC 1081 AGAACAGGTT TCTCCTGGAG CTACAGATAA GCAACAACAG GCTGGAGGAT GCGGGCGTGC 1141 GGGAGCTGTG CCAGGGCCTG GGCCAGCCTG GCTCTGTGCT GCGGGTGCTC TGGTTGGCCG 1201 ACTGCGATGT GAGTGACAGC AGCTGCAGCA GCCTCGCCGC AACCCTGTTG GCCAACCACA 1261 GCCTGCGTGA GCTGGACCTC AGCAACAACT GCCTGGGGGA CGCGGGCATC CTGCAGCTGG 1321 TGGAGAGCGT CCGGCAGCCG GGCTGCCTCC TGGAGCAGCT GGTCCTGTAC GACATTTACT 1381 GGTCTGAGGA GATGGAGGAC CGGCTGCAGG CCCTGGAGAA GGACAAGCCA TCCCTGAGGG 1441 TCATCTCCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1501 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1561 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGCCAC ACCACTTTAA TCCAGGAAAC 1621 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt