Construct: ORF ccsbBroad304_13723

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001770.1_s304c1, BRDN0000408600
Derived from:
ccsbBroadEn_13723
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
INTS4P2 (644619)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13723
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 644619 INTS4P2 integrator complex subunit ... NR_027392.2 74.4% 1_27del;1345_1768del
2 human 92105 INTS4 integrator complex subunit 4 XR_001748041.2 56.5% (many diffs)
3 human 92105 INTS4 integrator complex subunit 4 XM_011545352.2 48.2% 44.2% (many diffs)
4 human 92105 INTS4 integrator complex subunit 4 XR_002957209.1 47.5% (many diffs)
5 human 92105 INTS4 integrator complex subunit 4 XM_017018560.2 45.2% 41.5% (many diffs)
6 human 92105 INTS4 integrator complex subunit 4 NM_033547.4 42.8% 39.3% (many diffs)
7 human 92105 INTS4 integrator complex subunit 4 XM_011545353.3 42.2% 38% (many diffs)
8 human 285905 INTS4P1 integrator complex subunit ... NR_146905.1 40.5% (many diffs)
9 human 92105 INTS4 integrator complex subunit 4 XR_001748037.2 40.5% (many diffs)
10 human 92105 INTS4 integrator complex subunit 4 XR_001748036.2 39.7% (many diffs)
11 human 92105 INTS4 integrator complex subunit 4 XR_001748039.2 37.5% (many diffs)
12 human 92105 INTS4 integrator complex subunit 4 XR_001748040.2 36.5% (many diffs)
13 human 285905 INTS4P1 integrator complex subunit ... NR_146906.1 33.6% (many diffs)
14 human 92105 INTS4 integrator complex subunit 4 XR_002957208.1 23.6% (many diffs)
15 mouse 101861 Ints4 integrator complex subunit 4 XM_006507180.1 40.4% 38.8% (many diffs)
16 mouse 101861 Ints4 integrator complex subunit 4 XM_006507179.3 39.3% 38.4% (many diffs)
17 mouse 101861 Ints4 integrator complex subunit 4 NM_027256.2 39.2% 38.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1386
ORF length:
1317
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gcagcggccg ctgagggtgg cgcaggggcc ccggccagcc cggggctgca
121gcagtgcgga cagctccaga agctcatcgg catctccatt ggcagcctgc gcgggctggg
181caccaagtgc gctgtgtcca acgacctcac cgagcaggag atacggaccc tggagcattg
241tcccaattcc ttcttctaat gaagaaatac gcttagttga tgatgcgttt ggaaaaattt
301gtcacatggt cagtgatggc tcttgggtgg ttcatgttca ggcagcaaaa ctgttgggct
361ctatggagca agtcagttct catttcttgg agcagaccct tgacaagaag ctgatgtcag
421atctgaggag gaaacgtact gcacatgagc gtgccaagga actttacagt tcaggggagt
481tttccagtgg cagaaagtgg gaagatgatg ctcccaagga agaagtagat accggggctg
541tgaacttgat tgagtcagga gcttgtggag cttttgttca tgggttggaa gatgagatgt
601atgaggttcg tattgctgct gtggaggccc tctgcatgtt ggcccagtct tcaccctctt
661ttgctgagaa gtgccttgat ttcctagttg acatgttcaa cgatgaaatt gaggaagtac
721gtctgcagtc catacatacc atgagaaaaa tctctaacaa catcaccctc cgagaagatc
781agcttgacac tgtcctggct gtgctagagg attcagccag agatattcga gaggctcttc
841atgaactctt atgctgtact aatgtttcaa ccaaagaagg gattcatctt gcattggtgg
901agctgctgaa aaatttaacc aagtacccta ctgataggga ctccatatgg aagtgcttga
961agtttctggg aagtcggcat ccaaccctgg tgcttccctt ggtgccagag cttctgagca
1021cccacccatt ttttgacaca gctgaaccag acatggatga tccagcttat attgcagttt
1081tggtacttat tttcaatgct gctaaaacct gtccaacaat gcgagcattg ttctcagatc
1141acaccgtcag gcactatgcc tacctccgag acagtctttc tcatcttgtt cctgccttga
1201ggttaccagg tagaaaactg gtgtcatcag ctgtttctcc cagcatcata cctcaagagg
1261atccttcgca gcagttcctg cagcagagcc ttgaaagagt gtatagtctt cagcacttgg
1321accctcaggg agcccaggag ctgctggaat tcaccatcag gccttgcagg aaaagtcgtg
1381gaatgttgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
1441gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
1501acctctggat ta

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