Construct: ORF ccsbBroadEn_00683
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017508.1_s300c1, BRDN0000390378
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPS1 (2873)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2873 | GPS1 | G protein pathway suppressor 1 | NM_212492.3 | 100% | 100% | |
| 2 | human | 2873 | GPS1 | G protein pathway suppressor 1 | NM_001321090.2 | 94.9% | 94.9% | 1_84del |
| 3 | human | 2873 | GPS1 | G protein pathway suppressor 1 | NM_001321091.2 | 94.7% | 94.7% | 1_84del;889_890insAGC |
| 4 | human | 2873 | GPS1 | G protein pathway suppressor 1 | NM_001321089.2 | 94.2% | 94.2% | 1_84del;510_521del |
| 5 | human | 2873 | GPS1 | G protein pathway suppressor 1 | NM_001321092.2 | 91.8% | 90.8% | (many diffs) |
| 6 | human | 2873 | GPS1 | G protein pathway suppressor 1 | NM_001330539.2 | 91.6% | 90.7% | (many diffs) |
| 7 | human | 2873 | GPS1 | G protein pathway suppressor 1 | NM_004127.6 | 91.1% | 90.2% | (many diffs) |
| 8 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_024450718.1 | 91% | 91% | 1154_1231del;1586_1663del |
| 9 | human | 2873 | GPS1 | G protein pathway suppressor 1 | NM_001330541.2 | 90.9% | 90% | (many diffs) |
| 10 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_005256353.1 | 90.7% | 90.7% | 1_84del;1592_1669del |
| 11 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_024450717.1 | 90.7% | 90.7% | 1_84del;1238_1315del |
| 12 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_017024525.1 | 90.5% | 90.5% | 1_84del;889_890insAGC;1589_1666del |
| 13 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_005256350.1 | 90% | 90% | 1_84del;510_521del;1604_1681del |
| 14 | human | 2873 | GPS1 | G protein pathway suppressor 1 | NM_001321093.2 | 89.3% | 89.3% | 0_1ins168 |
| 15 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_017024535.2 | 89.1% | 89.1% | 0_1ins168;637_638insAGC |
| 16 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_024450728.1 | 89.1% | 89.1% | 0_1ins168;637_638insAGC |
| 17 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_017024534.1 | 88.7% | 88.7% | 0_1ins168;258_269del |
| 18 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_017024526.2 | 88.5% | 88.5% | 0_1ins168;258_269del;649_650insAGC |
| 19 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_005256360.4 | 87.5% | 86.6% | (many diffs) |
| 20 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_024450727.1 | 87.5% | 86.6% | (many diffs) |
| 21 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_017024527.2 | 87.3% | 86.4% | (many diffs) |
| 22 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_005256359.2 | 86.8% | 85.9% | (many diffs) |
| 23 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_024450716.1 | 86.8% | 86.8% | 1_84del;1238_1315del;1670_1747del |
| 24 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_024450715.1 | 86.2% | 86.2% | (many diffs) |
| 25 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_024450713.1 | 85.4% | 85.4% | 1_84del;890_1075del |
| 26 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_017024531.2 | 84.9% | 84.9% | 0_1ins168;637_638insAGC;1337_1414del |
| 27 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_017024524.2 | 84.3% | 84.3% | (many diffs) |
| 28 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_024450720.1 | 83.5% | 82.7% | (many diffs) |
| 29 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_024450721.1 | 83.4% | 82.5% | (many diffs) |
| 30 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_024450719.1 | 83% | 82.1% | (many diffs) |
| 31 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_024450725.1 | 81.3% | 81.3% | 0_1ins168;986_1063del;1418_1495del |
| 32 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_024450726.1 | 81.3% | 81.3% | 0_1ins168;986_1063del;1418_1495del |
| 33 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_024450722.1 | 80.7% | 80.7% | (many diffs) |
| 34 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_024450723.1 | 80.7% | 80.7% | (many diffs) |
| 35 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_024450714.1 | 80.6% | 80.6% | (many diffs) |
| 36 | human | 2873 | GPS1 | G protein pathway suppressor 1 | XM_024450724.1 | 80.6% | 80.6% | (many diffs) |
| 37 | mouse | 209318 | Gps1 | G protein pathway suppressor 1 | NM_145370.2 | 86.4% | 95.6% | (many diffs) |
| 38 | mouse | 209318 | Gps1 | G protein pathway suppressor 1 | XM_017314410.1 | 86.3% | 95.4% | (many diffs) |
| 39 | mouse | 209318 | Gps1 | G protein pathway suppressor 1 | XM_006532744.2 | 83.3% | 91.9% | (many diffs) |
| 40 | mouse | 209318 | Gps1 | G protein pathway suppressor 1 | XM_017314409.1 | 83.1% | 91.7% | (many diffs) |
| 41 | mouse | 209318 | Gps1 | G protein pathway suppressor 1 | NM_001177874.1 | 80.5% | 88.9% | (many diffs) |
| 42 | mouse | 209318 | Gps1 | G protein pathway suppressor 1 | XM_017314412.1 | 80.3% | 88.8% | (many diffs) |
| 43 | mouse | 209318 | Gps1 | G protein pathway suppressor 1 | NM_001346709.1 | 77.5% | 85.3% | (many diffs) |
| 44 | mouse | 209318 | Gps1 | G protein pathway suppressor 1 | XM_017314411.1 | 77.3% | 85.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1647
- ORF length:
- 1581
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAG GGATAGCTCG GCCCCCAGCT CGGCCTCCTC GTCAGTGACA GATCTGTACT 121 GCACCCCTCA CAGCAGTAGG TCAGACCTCG TCCTGCCCGG CACGGCCGGG GACTTCAGCC 181 TGAGCGCCAG CCTGTCGGCC TGTACGCTGC TCTACGAGGG GGCCGTGGAG CCCATGCAGA 241 TCGACGTGGA CCCCCAGGAA GACCCGCAGA ATGCACCTGA CGTCAACTAC GTGGTGGAGA 301 ACCCCAGCCT GGATCTGGAA CAGTACGCGG CCAGCTACAG CGGCCTGATG CGCATCGAAC 361 GGCTGCAGTT CATTGCTGAT CACTGCCCCA CGCTGCGGGT GGAGGCCCTG AAGATGGCCC 421 TCTCCTTCGT GCAGAGAACC TTTAACGTGG ACATGTACGA GGAGATCCAC CGCAAGCTCT 481 CAGAGGCCAC CAGGGAGCTG CAGAACGCAC CCGACGCCAT CCCTGAGAGC GGCGTGGAGC 541 CCCCAGCCCT GGACACGGCC TGGGTGGAGG CCACGCGGAA GAAGGCGCTG CTGAAGCTGG 601 AGAAGCTGGA CACAGACCTG AAGAACTACA AGGGCAACTC CATCAAAGAG AGCATCCGGC 661 GCGGCCACGA CGACCTGGGC GACCACTACC TGGACTGTGG GGACCTCAGC AACGCCCTCA 721 AGTGCTATTC CCGGGCCCGG GACTACTGCA CCAGCGCCAA ACACGTCATC AACATGTGCC 781 TCAATGTCAT CAAGGTCAGC GTCTACTTGC AGAATTGGTC TCATGTGCTC AGCTACGTCA 841 GCAAGGCTGA GTCCACCCCA GAGATTGCCG AGCAGCGAGG AGAGCGTGAC AGCCAGACCC 901 AGGCCATCCT CACCAAGCTC AAGTGTGCCG CAGGCTTGGC AGAGCTGGCC GCCAGGAAGT 961 ACAAGCAGGC TGCCAAGTGC CTCCTGCTGG CTTCCTTTGA TCACTGTGAC TTCCCTGAGC 1021 TGCTGTCCCC CAGCAACGTG GCCATCTACG GTGGCCTGTG CGCCTTGGCT ACCTTTGACC 1081 GGCAGGAGCT GCAGCGCAAT GTCATCTCCA GCAGCTCCTT CAAGTTGTTC TTGGAGCTGG 1141 AGCCACAGGT CCGAGACATC ATCTTCAAAT TCTACGAGTC CAAGTACGCC TCATGTCTCA 1201 AGATGCTGGA CGAGATGAAG GACAACCTGC TCCTGGACAT GTATCTGGCC CCCCATGTCA 1261 GGACCCTGTA CACCCAGATT CGCAACCGTG CCCTCATCCA GTATTTCAGC CCCTACGTGT 1321 CAGCCGACAT GCATAGGATG GCGGCAGCCT TCAATACCAC GGTGGCCGCC CTGGAGGACG 1381 AGCTGACGCA GCTAATCCTG GAGGGGCTGA TCAGTGCCCG TGTGGACTCA CACAGCAAGA 1441 TCCTATACGC CCGGGACGTG GATCAGCGCA GCACCACCTT TGAGAAGTCT CTGTTGATGG 1501 GCAAGGAGTT CCAGCGCCGC GCCAAGGCCA TGATGCTGCG GGCAGCTGTG CTCCGCAACC 1561 AGATCCATGT CAAGTCCCCG CCCAGAGAAG GGAGCCAGGG GGAGCTGACT CCAGCCAACA 1621 GCCAGTCCCG GATGAGCACC AACATGTGCC CAACTTTCTT GTACAAAGTt ggcattataa 1681 gaaagcattg cttatcaatt tgttgcaacg aac