Construct: ORF ccsbBroadEn_07469

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011988.1_s300c1, BRDN0000389843
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PUM1 (9698)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9698 PUM1 pumilio RNA binding family ... NM_014676.3 99.9% 100% 2337T>C
2 human 9698 PUM1 pumilio RNA binding family ... NM_001020658.2 99.8% 99.8% 2337T>C;2851_2856delGTAATT
3 mouse 80912 Pum1 pumilio RNA-binding family ... NM_001159603.1 93.9% 98.9% (many diffs)
4 mouse 80912 Pum1 pumilio RNA-binding family ... XM_017320459.1 93.8% 98.9% (many diffs)
5 mouse 80912 Pum1 pumilio RNA-binding family ... NM_001159604.1 93.7% 98.8% (many diffs)
6 mouse 80912 Pum1 pumilio RNA-binding family ... NM_030722.2 93.6% 98.7% (many diffs)
7 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539294.3 93.5% 98.5% (many diffs)
8 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539291.3 93.3% 98.4% (many diffs)
9 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539292.2 93.3% 98.4% (many diffs)
10 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539293.3 93.2% 98.3% (many diffs)
11 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539295.3 90.9% 95.9% (many diffs)
12 mouse 80912 Pum1 pumilio RNA-binding family ... XM_017320463.1 87.3% 92.6% (many diffs)
13 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539298.1 87.3% 92.5% (many diffs)
14 mouse 80912 Pum1 pumilio RNA-binding family ... XM_017320462.1 87.3% 92.5% (many diffs)
15 mouse 80912 Pum1 pumilio RNA-binding family ... XM_017320461.1 87.2% 92.5% (many diffs)
16 mouse 80912 Pum1 pumilio RNA-binding family ... XM_017320460.1 87.1% 92.4% (many diffs)
17 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539297.3 87% 92.2% (many diffs)
18 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539296.3 86.8% 92.1% (many diffs)
19 mouse 80912 Pum1 pumilio RNA-binding family ... XM_017320464.1 85.8% 90.8% (many diffs)
20 mouse 80912 Pum1 pumilio RNA-binding family ... NM_001159605.1 85.7% 90.7% (many diffs)
21 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539299.3 85.3% 90.3% (many diffs)
22 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539300.3 83.5% 88.3% (many diffs)
23 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539301.3 83.4% 88.2% (many diffs)
24 mouse 80912 Pum1 pumilio RNA-binding family ... XM_011250358.2 83.1% 86.9% (many diffs)
25 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539302.3 81.2% 86.1% (many diffs)
26 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539303.3 77.9% 79.3% (many diffs)
27 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539304.3 77.9% 79.3% (many diffs)
28 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539305.3 77.8% 79% (many diffs)
29 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539306.3 77.8% 79% (many diffs)
30 mouse 80912 Pum1 pumilio RNA-binding family ... XM_011250359.2 77.1% 78.8% (many diffs)
31 mouse 80912 Pum1 pumilio RNA-binding family ... XM_011250360.2 75.5% 80.3% (many diffs)
32 mouse 80912 Pum1 pumilio RNA-binding family ... NM_001159606.1 73.6% 78.5% (many diffs)
33 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539310.3 73.5% 78.4% (many diffs)
34 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539311.3 73.5% 78.4% (many diffs)
35 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539309.3 73.5% 78.3% (many diffs)
36 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539308.3 73.2% 78.1% (many diffs)
37 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539312.3 69.7% 73.1% (many diffs)
38 mouse 80912 Pum1 pumilio RNA-binding family ... XM_017320466.1 65.5% 70% (many diffs)
39 mouse 80912 Pum1 pumilio RNA-binding family ... XM_006539314.3 62.9% 66.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3624
ORF length:
3558
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAG CGTTGCATGT GTCTTGAAGA GAAAAGCAGT GCTTTGGCAG GACTCTTTCA
121GCCCCCACCT GAAACATCAC CCTCAAGAAC CAGCTAATCC CAACATGCCT GTTGTTTTGA
181CATCTGGAAC AGGGTCGCAA GCGCAGCCAC AACCAGCTGC AAATCAGGCT CTTGCAGCTG
241GGACTCACTC CAGCCCTGTC CCAGGATCTA TAGGAGTTGC AGGCCGTTCC CAGGACGACG
301CTATGGTGGA CTACTTCTTT CAGAGGCAGC ATGGTGAGCA GCTTGGGGGA GGAGGAAGTG
361GAGGAGGCGG CTATAATAAT AGCAAACATC GATGGCCTAC TGGGGATAAC ATTCATGCAG
421AACATCAGGT GCGTTCCATG GATGAACTGA ATCATGATTT TCAAGCACTT GCTCTGGAGG
481GAAGAGCGAT GGGAGAGCAG CTCTTGCCAG GTAAAAAGTT TTGGGAAACA GATGAATCCA
541GCAAAGATGG ACCAAAAGGA ATATTCCTGG GTGATCAATG GCGAGACAGT GCCTGGGGAA
601CATCAGATCA TTCAGTTTCC CAGCCAATCA TGGTGCAGAG AAGACCTGGT CAGAGTTTCC
661ATGTGAACAG TGAGGTCAAT TCTGTACTGT CCCCACGATC GGAGAGTGGG GGACTAGGCG
721TTAGCATGGT GGAGTATGTG TTGAGCTCAT CCCCGGGCGA TTCCTGTCTA AGAAAAGGAG
781GATTTGGCCC AAGGGATGCA GACAGTGATG AAAACGACAA AGGTGAAAAG AAGAACAAGG
841GTACGTTTGA TGGAGATAAG CTAGGAGATT TGAAGGAGGA GGGTGATGTG ATGGACAAGA
901CCAATGGTTT ACCAGTGCAG AATGGGATTG ATGCAGACGT CAAAGATTTT AGCCGTACCC
961CTGGTAATTG CCAGAACTCT GCTAATGAAG TGGATCTTCT GGGTCCAAAC CAGAATGGTT
1021CTGAGGGCTT AGCCCAGCTG ACCAGCACCA ATGGTGCCAA GCCTGTGGAG GATTTCTCCA
1081ACATGGAGTC CCAGAGTGTC CCCTTGGACC CCATGGAACA TGTGGGCATG GAGCCTCTTC
1141AGTTTGATTA TTCAGGCACG CAGGTACCTG TGGACTCAGC AGCAGCAACT GTGGGACTTT
1201TTGACTACAA TTCTCAACAA CAGCTGTTCC AAAGACCTAA TGCGCTTGCT GTCCAGCAGT
1261TGACAGCTGC TCAGCAGCAG CAGTATGCAC TGGCAGCTGC TCATCAGCCG CACATCGGTT
1321TAGCTCCCGC TGCGTTTGTC CCCAATCCAT ACATCATCAG CGCTGCTCCC CCAGGGACGG
1381ACCCCTACAC AGCTGGATTG GCTGCAGCAG CGACACTAGG CCCAGCTGTG GTCCCTCACC
1441AGTATTATGG AGTTACTCCC TGGGGAGTCT ACCCTGCCAG TCTTTTCCAG CAGCAAGCTG
1501CCGCTGCCGC TGCAGCAACT AATTCAGCTA ATCAACAGAC CACCCCACAG GCTCAGCAAG
1561GACAGCAGCA GGTTCTCCGT GGAGGAGCCA GCCAACGTCC TTTGACCCCA AACCAGAACC
1621AGCAGGGACA GCAAACGGAT CCCCTTGTGG CAGCTGCAGC AGTGAATTCT GCCCTTGCAT
1681TTGGACAAGG TCTGGCAGCA GGCATGCCAG GTTATCCGGT GTTGGCTCCT GCTGCTTACT
1741ATGACCAAAC TGGTGCCCTT GTAGTGAATG CAGGCGCGAG AAATGGTCTT GGAGCTCCTG
1801TTCGACTTGT AGCTCCTGCC CCAGTCATCA TTAGTTCCTC AGCTGCACAA GCAGCTGTTG
1861CAGCAGCCGC AGCTTCAGCA AATGGAGCAG CTGGTGGTCT TGCTGGAACA ACAAATGGAC
1921CATTTCGCCC TTTAGGAACA CAGCAGCCTC AGCCCCAGCC CCAGCAGCAG CCCAATAACA
1981ACCTGGCATC CAGTTCTTTC TACGGCAACA ACTCTCTGAA CAGCAATTCA CAGAGCAGCT
2041CCCTCTTCTC CCAGGGCTCT GCCCAGCCTG CCAACACATC CTTGGGATTC GGAAGTAGCA
2101GTTCTCTCGG CGCCACCCTG GGATCCGCCC TTGGAGGGTT TGGAACAGCA GTTGCAAACT
2161CCAACACTGG CAGTGGCTCC CGCCGTGACT CCCTGACTGG CAGCAGTGAC CTTTATAAGA
2221GGACATCGAG CAGCTTGACC CCCATTGGAC ACAGTTTTTA TAACGGCCTT AGCTTTTCCT
2281CCTCTCCTGG ACCCGTGGGC ATGCCTCTCC CTAGTCAGGG ACCAGGACAT TCACAGACAC
2341CACCTCCTTC CCTCTCTTCA CATGGATCCT CTTCAAGCTT AAACCTGGGA GGACTCACGA
2401ACGGCAGTGG AAGATACATC TCTGCTGCTC CAGGCGCTGA AGCCAAGTAC CGCAGTGCAA
2461GCAGCGCCTC CAGCCTCTTC AGCCCGAGCA GCACTCTTTT CTCTTCCTCT CGTTTGCGAT
2521ATGGAATGTC TGATGTCATG CCTTCTGGCA GGAGCAGGCT TTTGGAAGAT TTTCGAAACA
2581ACCGGTACCC CAATTTACAA CTGCGGGAGA TTGCTGGACA TATAATGGAA TTTTCCCAAG
2641ACCAGCATGG GTCCAGATTC ATTCAGCTGA AACTGGAGCG TGCCACACCA GCTGAGCGCC
2701AGCTTGTCTT CAATGAAATC CTCCAGGCTG CCTACCAACT CATGGTGGAT GTGTTTGGTA
2761ATTACGTCAT TCAGAAGTTC TTTGAATTTG GCAGTCTTGA ACAGAAGCTG GCTTTGGCAG
2821AACGGATTCG AGGCCACGTC CTGTCATTGG CACTACAGAT GTATGGCTGC CGTGTTATCC
2881AGAAAGCTCT TGAGTTTATT CCTTCAGACC AGCAGAATGA GATGGTTCGG GAACTAGATG
2941GCCATGTCTT GAAGTGTGTG AAAGATCAGA ATGGCAATCA CGTGGTTCAG AAATGCATTG
3001AATGTGTACA GCCCCAGTCT TTGCAATTTA TCATCGATGC GTTTAAGGGA CAGGTATTTG
3061CCTTATCCAC ACATCCTTAT GGCTGCCGAG TGATTCAGAG AATCCTGGAG CACTGTCTCC
3121CTGACCAGAC ACTCCCTATT TTAGAGGAGC TTCACCAGCA CACAGAGCAG CTTGTACAGG
3181ATCAATATGG AAATTATGTA ATCCAACATG TACTGGAGCA CGGTCGTCCT GAGGATAAAA
3241GCAAAATTGT AGCAGAAATC CGAGGCAATG TACTTGTATT GAGTCAGCAC AAATTTGCAA
3301GCAATGTTGT GGAGAAGTGT GTTACTCACG CCTCACGTAC GGAGCGCGCT GTGCTCATCG
3361ATGAGGTGTG CACCATGAAC GACGGTCCCC ACAGTGCCTT ATACACCATG ATGAAGGACC
3421AGTATGCCAA CTACGTGGTC CAGAAGATGA TTGACGTGGC GGAGCCAGGC CAGCGGAAGA
3481TCGTCATGCA TAAGATCCGG CCCCACATCG CAACTCTTCG TAAGTACACC TATGGCAAGC
3541ACATTCTGGC CAAGCTGGAG AAGTACTACA TGAAGAACGG TGTTGACTTA GGGCCCATCT
3601GTGGCCCCCC TAATGGTATC ATCTGCCCAA CTTTCTTGTA CAAAGTtggc attataagaa
3661agcattgctt atcaatttgt tgcaacgaac

Download FASTA (ORF) (Full)