Transcript: Human NM_001351774.2

Homo sapiens zinc finger protein 320 (ZNF320), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
ZNF320 (162967)
Length:
6331
CDS:
386..1915

Additional Resources:

NCBI RefSeq record:
NM_001351774.2
NBCI Gene record:
ZNF320 (162967)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001351774.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000230180 ATTCACCTTCACACCTTATTA pLKO_005 1737 CDS 100% 15.000 21.000 N ZNF320 n/a
2 TRCN0000218471 GGTCAGCTTGAATCAAGATTT pLKO_005 797 CDS 100% 13.200 18.480 N ZNF320 n/a
3 TRCN0000230179 CGTACCTCGCATGTCATCAAA pLKO_005 1578 CDS 100% 5.625 4.500 N ZNF320 n/a
4 TRCN0000156757 GCTCGAAATTCAGTCCTCGTA pLKO.1 1232 CDS 100% 2.640 2.112 N ZNF320 n/a
5 TRCN0000219853 ACTGATACAAATCCGTTATAA pLKO.1 3379 3UTR 100% 15.000 10.500 N ZNF320 n/a
6 TRCN0000230181 ACTGATACAAATCCGTTATAA pLKO_005 3379 3UTR 100% 15.000 10.500 N ZNF320 n/a
7 TRCN0000230178 TTTGCATTTGCGAAGACATAG pLKO_005 820 CDS 100% 10.800 7.560 N ZNF320 n/a
8 TRCN0000219854 TTGCTTCCCTTGCCCTATTTA pLKO.1 5025 3UTR 100% 15.000 9.000 N ZNF320 n/a
9 TRCN0000155388 CCCTTGCATGTCATCATAGAA pLKO.1 1077 CDS 100% 5.625 3.375 N ZNF320 n/a
10 TRCN0000152095 CTGGAGAGAAACTTTACGAAT pLKO.1 1608 CDS 100% 4.950 2.970 N ZNF320 n/a
11 TRCN0000157621 GCATGATTCACACCTGGCAAA pLKO.1 982 CDS 100% 4.050 2.430 N ZNF320 n/a
12 TRCN0000021905 GACGTGATGCTGGAGAATTAT pLKO.1 488 CDS 100% 15.000 7.500 Y ZNF765 n/a
13 TRCN0000018107 GCTGGAAACAAGCCTATTAAA pLKO.1 776 CDS 100% 15.000 7.500 Y ZNF600 n/a
14 TRCN0000021906 CCCTGCTCAGAGGACTCTATA pLKO.1 463 CDS 100% 13.200 6.600 Y ZNF765 n/a
15 TRCN0000147302 GTCGCAAATCACATCTTGAAA pLKO.1 1401 CDS 100% 5.625 2.813 Y ZNF321P n/a
16 TRCN0000015887 GTGAAGAATGTGACAAAGTTT pLKO.1 1629 CDS 100% 5.625 2.813 Y ZNF702P n/a
17 TRCN0000021908 TCAGGGATGTGGCCATAGAAT pLKO.1 414 CDS 100% 5.625 2.813 Y ZNF765 n/a
18 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 2030 3UTR 100% 4.950 2.475 Y CFLAR n/a
19 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 2030 3UTR 100% 4.950 2.475 Y C19orf31 n/a
20 TRCN0000021816 CTGGAGAGAAACCTTACAGAT pLKO.1 1356 CDS 100% 4.950 2.475 Y ZNF253 n/a
21 TRCN0000155654 CTGGAGAGAAACCTTACAGAT pLKO.1 1356 CDS 100% 4.950 2.475 Y ZNF320 n/a
22 TRCN0000154986 GCAAGACCTTTAGTCAGACAT pLKO.1 1137 CDS 100% 4.950 2.475 Y ZNF320 n/a
23 TRCN0000142662 GCAAGGCAATACAGAAGTGAT pLKO.1 571 CDS 100% 4.950 2.475 Y ZNF468 n/a
24 TRCN0000154835 GCAGAACATCAGAGAGTTCAT pLKO.1 1502 CDS 100% 4.950 2.475 Y ZNF320 n/a
25 TRCN0000150214 GTAATGAATGTGGCAAGGTTT pLKO.1 1041 CDS 100% 4.950 2.475 Y ZNF813 n/a
26 TRCN0000151709 CAAATGTAATGAGTGTGGCAA pLKO.1 1204 CDS 100% 2.640 1.320 Y ZNF320 n/a
27 TRCN0000021907 GCTGGAGAATTATAGGAACCT pLKO.1 496 CDS 100% 2.640 1.320 Y ZNF765 n/a
28 TRCN0000142023 GTAAGGTTTGTGACAAGGCTT pLKO.1 1461 CDS 100% 2.640 1.320 Y ZNF468 n/a
29 TRCN0000219000 ACTGCAGAGAAACCCTATAAA pLKO_005 1271 CDS 100% 15.000 7.500 Y ZNF443 n/a
30 TRCN0000235273 ACTGGAGAGAAACCTTATAAA pLKO_005 1103 CDS 100% 15.000 7.500 Y Gm10771 n/a
31 TRCN0000337271 ACTGGAGAGAAACCTTATAAA pLKO_005 1103 CDS 100% 15.000 7.500 Y ZNF286B n/a
32 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 5735 3UTR 100% 13.200 6.600 Y LIAS n/a
33 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 2028 3UTR 100% 4.950 2.475 Y ERN2 n/a
34 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 2028 3UTR 100% 4.950 2.475 Y P3H4 n/a
35 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 2028 3UTR 100% 4.950 2.475 Y P3H4 n/a
36 TRCN0000158848 GAGAAACCTTACAAATGTGAT pLKO.1 857 CDS 100% 4.950 2.475 Y ZNF28 n/a
37 TRCN0000235274 ATACTGGAGAGAGACCTTATG pLKO_005 1521 CDS 100% 10.800 5.400 Y Gm10771 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001351774.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05120 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_05120 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000475531 CGCGGTATGATTAGCATATCCGAT pLX_317 14.1% 100% 100% V5 n/a
4 ccsbBroadEn_13646 pDONR223 100% 11.3% 1.4% None (many diffs) n/a
5 ccsbBroad304_13646 pLX_304 0% 11.3% 1.4% V5 (many diffs) n/a
6 TRCN0000473343 CGTTTTTTTACCTCATATCTGGTT pLX_317 100% 11.3% 1.4% V5 (many diffs) n/a
7 ccsbBroadEn_14339 pDONR223 100% 11.2% .5% None (many diffs) n/a
8 ccsbBroad304_14339 pLX_304 0% 11.2% .5% V5 (not translated due to prior stop codon) (many diffs) n/a
9 TRCN0000478280 ACGAGTTCACTGTCATGACCAACC pLX_317 100% 11.2% .5% V5 (not translated due to prior stop codon) (many diffs) n/a
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