Construct: ORF TRCN0000469026
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001770.1_s317c1
- Derived from:
- ccsbBroadEn_13723
- DNA Barcode:
- TTGCGCAAGTTGCCCGAAACCAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- INTS4P2 (644619)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469026
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 644619 | INTS4P2 | integrator complex subunit ... | NR_027392.2 | 74.4% | 1_27del;1345_1768del | |
| 2 | human | 92105 | INTS4 | integrator complex subunit 4 | XR_001748041.2 | 56.5% | (many diffs) | |
| 3 | human | 92105 | INTS4 | integrator complex subunit 4 | XM_011545352.2 | 48.2% | 44.2% | (many diffs) |
| 4 | human | 92105 | INTS4 | integrator complex subunit 4 | XR_002957209.1 | 47.5% | (many diffs) | |
| 5 | human | 92105 | INTS4 | integrator complex subunit 4 | XM_017018560.2 | 45.2% | 41.5% | (many diffs) |
| 6 | human | 92105 | INTS4 | integrator complex subunit 4 | NM_033547.4 | 42.8% | 39.3% | (many diffs) |
| 7 | human | 92105 | INTS4 | integrator complex subunit 4 | XM_011545353.3 | 42.2% | 38% | (many diffs) |
| 8 | human | 285905 | INTS4P1 | integrator complex subunit ... | NR_146905.1 | 40.5% | (many diffs) | |
| 9 | human | 92105 | INTS4 | integrator complex subunit 4 | XR_001748037.2 | 40.5% | (many diffs) | |
| 10 | human | 92105 | INTS4 | integrator complex subunit 4 | XR_001748036.2 | 39.7% | (many diffs) | |
| 11 | human | 92105 | INTS4 | integrator complex subunit 4 | XR_001748039.2 | 37.5% | (many diffs) | |
| 12 | human | 92105 | INTS4 | integrator complex subunit 4 | XR_001748040.2 | 36.5% | (many diffs) | |
| 13 | human | 285905 | INTS4P1 | integrator complex subunit ... | NR_146906.1 | 33.6% | (many diffs) | |
| 14 | human | 92105 | INTS4 | integrator complex subunit 4 | XR_002957208.1 | 23.6% | (many diffs) | |
| 15 | mouse | 101861 | Ints4 | integrator complex subunit 4 | XM_006507180.1 | 40.4% | 38.8% | (many diffs) |
| 16 | mouse | 101861 | Ints4 | integrator complex subunit 4 | XM_006507179.3 | 39.3% | 38.4% | (many diffs) |
| 17 | mouse | 101861 | Ints4 | integrator complex subunit 4 | NM_027256.2 | 39.2% | 38.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1386
- ORF length:
- 1317
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagcggccg ctgagggtgg cgcaggggcc ccggccagcc cggggctgca 121 gcagtgcgga cagctccaga agctcatcgg catctccatt ggcagcctgc gcgggctggg 181 caccaagtgc gctgtgtcca acgacctcac cgagcaggag atacggaccc tggagcattg 241 tcccaattcc ttcttctaat gaagaaatac gcttagttga tgatgcgttt ggaaaaattt 301 gtcacatggt cagtgatggc tcttgggtgg ttcatgttca ggcagcaaaa ctgttgggct 361 ctatggagca agtcagttct catttcttgg agcagaccct tgacaagaag ctgatgtcag 421 atctgaggag gaaacgtact gcacatgagc gtgccaagga actttacagt tcaggggagt 481 tttccagtgg cagaaagtgg gaagatgatg ctcccaagga agaagtagat accggggctg 541 tgaacttgat tgagtcagga gcttgtggag cttttgttca tgggttggaa gatgagatgt 601 atgaggttcg tattgctgct gtggaggccc tctgcatgtt ggcccagtct tcaccctctt 661 ttgctgagaa gtgccttgat ttcctagttg acatgttcaa cgatgaaatt gaggaagtac 721 gtctgcagtc catacatacc atgagaaaaa tctctaacaa catcaccctc cgagaagatc 781 agcttgacac tgtcctggct gtgctagagg attcagccag agatattcga gaggctcttc 841 atgaactctt atgctgtact aatgtttcaa ccaaagaagg gattcatctt gcattggtgg 901 agctgctgaa aaatttaacc aagtacccta ctgataggga ctccatatgg aagtgcttga 961 agttTCTGGG AAGTCGGCAT CCAACCCTGG TGCTTCCCTT GGTGCCAGAG CTTCTGAGCA 1021 CCCACCCATT TTTTGACACA GCTGAACCAG ACATGGATGA TCCAGCTTAT ATTGCAGTTT 1081 TGGTACTTAT TTTCAATGCT GCTAAAACCT GTCCAACAAT GCGAGCATTG TTCTCAGATC 1141 ACACCGTCAG GCACTATGCC TACCTCCGAG ACAGTCTTTC TCATCTTGTT CCTGCCTTGA 1201 GGTTACCAGG TAGAAAACTG GTGTCATCAG CTGTTTCTCC CAGCATCATA CCTCAAGAGG 1261 ATCCTTCGCA GCAGTTCCTG CAGCAGAGCC TTGAAAGAGT GTATAGTCTT CAGCACTTGG 1321 ACCCTCAGGG AGCCCAGGAG CTGCTGGAAT TCACCATCAG GCCTTGCAGG AAAAGTCGTG 1381 GAATGTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1441 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1501 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTGCGCAAG TTGCCCGAAA CCAACACGCG 1561 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt