Construct: ORF TRCN0000470961
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016439.1_s317c1
- Derived from:
- ccsbBroadEn_12974
- DNA Barcode:
- GAATTATTCATAGACCCCCAATAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- INTS4 (92105)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470961
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92105 | INTS4 | integrator complex subunit 4 | XM_011545353.3 | 98.7% | 98.8% | 1515_1533delinsG |
| 2 | human | 92105 | INTS4 | integrator complex subunit 4 | XR_001748041.2 | 69.4% | 1_24del;1540_2182del | |
| 3 | human | 92105 | INTS4 | integrator complex subunit 4 | XR_002957209.1 | 59.2% | 1_24del;1540_2555del | |
| 4 | human | 92105 | INTS4 | integrator complex subunit 4 | XM_017018560.2 | 55.4% | 55.4% | 1516_2730del |
| 5 | human | 92105 | INTS4 | integrator complex subunit 4 | NM_033547.4 | 52.4% | 52.4% | 1516_2889del |
| 6 | human | 92105 | INTS4 | integrator complex subunit 4 | XR_001748037.2 | 49.6% | 1_24del;1540_3054del | |
| 7 | human | 92105 | INTS4 | integrator complex subunit 4 | XR_001748036.2 | 48.6% | 1_24del;1540_3114del | |
| 8 | human | 92105 | INTS4 | integrator complex subunit 4 | XR_001748039.2 | 46.1% | 1_24del;1540_3286del | |
| 9 | human | 92105 | INTS4 | integrator complex subunit 4 | XR_001748040.2 | 44.9% | 1_24del;1540_3372del | |
| 10 | human | 92105 | INTS4 | integrator complex subunit 4 | XM_011545352.2 | 41% | 41% | 0_1ins330;1186_2559del |
| 11 | human | 644619 | INTS4P2 | integrator complex subunit ... | NR_027392.2 | 35% | (many diffs) | |
| 12 | human | 92105 | INTS4 | integrator complex subunit 4 | XR_002957208.1 | 28.8% | 1_24del;1540_5259del | |
| 13 | human | 285905 | INTS4P1 | integrator complex subunit ... | NR_146905.1 | 25.9% | (many diffs) | |
| 14 | human | 285905 | INTS4P1 | integrator complex subunit ... | NR_146906.1 | 23.8% | (many diffs) | |
| 15 | mouse | 101861 | Ints4 | integrator complex subunit 4 | NM_027256.2 | 47.9% | 51.5% | (many diffs) |
| 16 | mouse | 101861 | Ints4 | integrator complex subunit 4 | XM_006507179.3 | 47.9% | 51.6% | (many diffs) |
| 17 | mouse | 101861 | Ints4 | integrator complex subunit 4 | XM_006507180.1 | 47% | 50.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1581
- ORF length:
- 1515
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgcacctt aagaagcggg tttatgagga attcacgaaa gtggttcagc 121 cacaggagga aattgctact aagaaactcc gactaacaaa accaagtaaa tctgcagcac 181 tccacataga tctgtgtaaa gctacctccc cagcagatgc tttgcaatac ttgctccagt 241 ttgccaggaa gcctgtcgag gcggaaagcg tagagggagt agtcaggatt ctcttggaac 301 attattacaa ggagaatgat ccatctgtga gactgaaaat tgcatcattg ttgggtttat 361 tatcaaagac agcaggattt tcaccagact gcattatgga tgatgccatc aacatcctgc 421 agaatgaaaa gtctcatcaa gtcctagctc aactgctgga tactttgctt gcaattggca 481 ctaagctacc agagaatcaa gctatccaaa tgcgattagt tgatgtggcc tgcaagcatc 541 tgacagatac gtctcatggt gtaagaaata agtgcctgca gttacttggc aatcttggct 601 ctttggagaa aagtgtcaca aaagatgcag aaggcctagc tgccagagat gtccagaaga 661 ttatagggga ttacttcagt gaccaagacc cacgtgtcag aacagcagct ataaaagcca 721 tgttgcagct ccatgaaaga ggactgaaat tacaccaaac aatttataat caggcctgta 781 aattactctc tgatgactat gaacaagtgc gcagtgctgc agtccagctt atctgggtcg 841 tcagtcagct ctatcctgaa agcattgtcc caattccttc ttctaatgaa gaaatacgct 901 tagttgatga tgcgtttggc aaaatttgtc acatggtcag tgatggctct tgggtggttc 961 gtgttcaggc agcaaaactg ttgggctcta tggagcaagt cagttctcat ttcttggagc 1021 agacccttga caagaagctg atgtcagatc tgaggaggaa acgtactgca catgagcgtg 1081 ccaaggaact ttacagttcg ggggagtttt ccagtggcag aaagtgggGA GATGATGCTC 1141 CCAAGGAAGA AGTAGATACC GGGGCTGTGA ACTTGATTGA GTCAGGAGCT TGTGGAGCTT 1201 TTGTTCATGG GTTGGAAGAT GAGATGTATG AGGTTCGTAT TGCTGCTGTG GAGGCCCTCT 1261 GCATGTTGGC CCAGTCTTCA CCCTCTTTTG CTGAGAAGTG CCTTGATTTC CTAGTTGACA 1321 TGTTCAACGA TGAAATTGAG GAAGTACGTC TGCAGTCTAT ACATACCATG AGAAAAATCT 1381 CTAACAACAT CACCCTCCGA GAAGATCAGC TTGACACTGT CCTGGCTGTG CTAGAGGATT 1441 CATCCAGAGA TATTCGAGAG GCTCTTCATG AACTCTTATG CTGTACTAAT GTTTCAACCA 1501 AAGAAGGGAT TCATCTTGCA TTGGTGGAGC TGCTGAAAAA TTTAACCAAG TACCCTACTG 1561 ATAGGGACTC CATATGGAAG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1621 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1681 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGAAT TATTCATAGA 1741 CCCCCAATAG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt