Construct: ORF TRCN0000472761
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015895.1_s317c1
- Derived from:
- ccsbBroadEn_15273
- DNA Barcode:
- GCGGTTCAATGTTGTAGTCTTGTG
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF100 (163227)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472761
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 148203 | ZNF738 | zinc finger protein 738 | NM_001355237.2 | 61.4% | 52.1% | (many diffs) |
| 2 | human | 163227 | ZNF100 | zinc finger protein 100 | NM_173531.4 | 48.4% | 48.5% | 789_1626del |
| 3 | human | 163227 | ZNF100 | zinc finger protein 100 | NM_001351669.2 | 48.2% | 48.3% | 222_223insGCA;786_1623del |
| 4 | human | 163227 | ZNF100 | zinc finger protein 100 | NM_001351670.2 | 42.3% | 42.4% | 222_223ins99;690_1527del |
| 5 | human | 80264 | ZNF430 | zinc finger protein 430 | NM_025189.4 | 40.8% | 35.6% | (many diffs) |
| 6 | human | 80264 | ZNF430 | zinc finger protein 430 | NM_001172671.2 | 40.7% | 35.4% | (many diffs) |
| 7 | human | 170959 | ZNF431 | zinc finger protein 431 | NM_001319124.2 | 39.6% | 33.9% | (many diffs) |
| 8 | human | 170959 | ZNF431 | zinc finger protein 431 | NM_133473.4 | 39.4% | 33.7% | (many diffs) |
| 9 | human | 148203 | ZNF738 | zinc finger protein 738 | NM_001355240.1 | 39.3% | 33.3% | (many diffs) |
| 10 | human | 170959 | ZNF431 | zinc finger protein 431 | XM_011527750.2 | 38.5% | 33% | (many diffs) |
| 11 | human | 148203 | ZNF738 | zinc finger protein 738 | NM_001355241.2 | 36.8% | 32.6% | (many diffs) |
| 12 | human | 646864 | ZNF723 | zinc finger protein 723 | NM_001349726.2 | 36.4% | 30.6% | (many diffs) |
| 13 | human | 646864 | ZNF723 | zinc finger protein 723 | XM_024451652.1 | 36.4% | 30.6% | (many diffs) |
| 14 | human | 646864 | ZNF723 | zinc finger protein 723 | XM_024451653.1 | 34.3% | 28.8% | (many diffs) |
| 15 | human | 284443 | ZNF493 | zinc finger protein 493 | NM_145326.3 | 32.7% | 27.1% | (many diffs) |
| 16 | human | 440519 | ZNF724 | zinc finger protein 724 | NM_001355404.2 | 29.8% | 25.5% | (many diffs) |
| 17 | human | 163227 | ZNF100 | zinc finger protein 100 | NM_001351671.2 | 29.8% | 29.8% | 0_1ins303;486_1323del |
| 18 | human | 440519 | ZNF724 | zinc finger protein 724 | NM_001355406.2 | 29% | 24.7% | (many diffs) |
| 19 | human | 57615 | ZNF492 | zinc finger protein 492 | NM_020855.3 | 28.5% | 24% | (many diffs) |
| 20 | human | 7639 | ZNF85 | zinc finger protein 85 | NM_001256172.2 | 28.1% | 23.8% | (many diffs) |
| 21 | human | 148206 | ZNF714 | zinc finger protein 714 | NM_182515.4 | 28.1% | 22.2% | (many diffs) |
| 22 | human | 440519 | ZNF724 | zinc finger protein 724 | XM_024451521.1 | 26.6% | 24.7% | (many diffs) |
| 23 | human | 284443 | ZNF493 | zinc finger protein 493 | NM_001076678.3 | 26.2% | 22% | (many diffs) |
| 24 | human | 51427 | ZNF107 | zinc finger protein 107 | XM_024446791.1 | 25.8% | 23.9% | (many diffs) |
| 25 | human | 170959 | ZNF431 | zinc finger protein 431 | XR_001753621.1 | 25.5% | (many diffs) | |
| 26 | human | 730087 | ZNF726 | zinc finger protein 726 | NM_001348689.2 | 25.3% | 22.4% | (many diffs) |
| 27 | human | 148206 | ZNF714 | zinc finger protein 714 | NR_117087.1 | 24.6% | (many diffs) | |
| 28 | human | 148206 | ZNF714 | zinc finger protein 714 | NR_117086.1 | 24.3% | (many diffs) | |
| 29 | human | 170959 | ZNF431 | zinc finger protein 431 | NM_001319126.2 | 24.1% | 20.2% | (many diffs) |
| 30 | human | 170959 | ZNF431 | zinc finger protein 431 | NM_001319127.2 | 23.2% | 19.9% | (many diffs) |
| 31 | human | 7697 | ZNF138 | zinc finger protein 138 | NM_001271637.2 | 20.9% | 15.2% | (many diffs) |
| 32 | human | 170959 | ZNF431 | zinc finger protein 431 | XR_001753620.1 | 13.6% | (many diffs) | |
| 33 | human | 400682 | LOC400682 | zinc finger protein 100-like | NR_144514.1 | 12.5% | (many diffs) | |
| 34 | human | 284443 | ZNF493 | zinc finger protein 493 | NM_175910.7 | 12.2% | 10.2% | (many diffs) |
| 35 | human | 170959 | ZNF431 | zinc finger protein 431 | NR_138053.2 | 7.9% | (many diffs) | |
| 36 | human | 170959 | ZNF431 | zinc finger protein 431 | NR_138052.2 | 7.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 855
- ORF length:
- 789
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgacccgagg tatggaatgt gtcctctcaa gggagcaagt ggatgccctg 121 gggctgagag gagtcttctg gtgcagtctt attttgaaaa ggggccattg acgtttaggg 181 atgtggccat agaattctct ctggaggagt ggcaatgcct ggacagtgct cagcagggtt 241 tgtataggaa agtgatgtta gagaactaca gaaacctggt cttcttggca ggtattgctc 301 tcactaagcc agacctgatc acctgtctgg agcaaggaaa agagccctGG AATATAAAGA 361 GACATGAGAT GGTAGCCAAA CCCCCAGTTA TATGTTCTCA TTTTCCCCAA GACCTTTGGG 421 CAGAGCAGGA CATTAAAGAT TCTTTTCAAG AAGCGATTCT GAAAAAATAT GGAAAATATG 481 GACATGACAA TTTACAGTTA CAAAAAGGCT GTAAAAGTGT GGATGAGTGT AAAGTGCACA 541 AAGAACATGA TAACAAATTA AACCAGTGTT TGATAACTAC CCAGAGCAAC ATATTTCAAT 601 GTGATCCATC TGCAAAAGTC TTTCATACAT TTTCAAATTC AAACAGACAT AAGATAAGAC 661 ATACTAGAAA GAAACCTTTC AAATGTAAAA AATGTGAAAA ATCATTTTGC ATGCTTTTAC 721 ACCTAACTCA ACATAAAAGA TTTCATATTA CAGAGAATTC CTACCAATGT AAAGATTGTG 781 GCAAAGCCTT CAACTGGTTC TCAACCCTTA CTACACACAG GAGAATTCAT ACTGGAGAGA 841 AACCCTACAA ATGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC 901 TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT 961 TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG CGGTTCAATG TTGTAGTCTT 1021 GTGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att