Transcript: Human NM_001319127.2

Homo sapiens zinc finger protein 431 (ZNF431), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
ZNF431 (170959)
Length:
13974
CDS:
546..1955

Additional Resources:

NCBI RefSeq record:
NM_001319127.2
NBCI Gene record:
ZNF431 (170959)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001319127.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000016484 CCGCAAGTGTAGATGAGTATA pLKO.1 667 CDS 100% 13.200 18.480 N ZNF431 n/a
2 TRCN0000016485 GTGAAGAATGTGACAATACAT pLKO.1 1849 CDS 100% 5.625 3.938 N ZNF431 n/a
3 TRCN0000016486 CCCACAACTTACTGCACATAA pLKO.1 1544 CDS 100% 13.200 7.920 N ZNF431 n/a
4 TRCN0000016483 GCTGAGAGGAATCTTCTAGTT pLKO.1 202 5UTR 100% 4.950 2.970 N ZNF431 n/a
5 TRCN0000430017 CCAGTCCTCAAACCTTATTAA pLKO_005 1874 CDS 100% 15.000 7.500 Y ZNF431 n/a
6 TRCN0000427608 GTGCACAAAGAAGGTTATAAT pLKO_005 690 CDS 100% 15.000 7.500 Y ZNF431 n/a
7 TRCN0000236731 ACCTTACTACACATAAGATAA pLKO_005 1129 CDS 100% 13.200 6.600 Y ZNF98 n/a
8 TRCN0000236730 CCCTTACTACACATAAGATAA pLKO_005 1045 CDS 100% 13.200 6.600 Y ZNF98 n/a
9 TRCN0000204533 CCTCCCAAAGTGCTGGAATTA pLKO.1 10341 3UTR 100% 13.200 6.600 Y LRRC74B n/a
10 TRCN0000096537 CTGGAGAGAAACCCTACAAAT pLKO.1 1240 CDS 100% 13.200 6.600 Y Zfp934 n/a
11 TRCN0000235358 CTGGAGAGAAACCCTACAAAT pLKO_005 1240 CDS 100% 13.200 6.600 Y 2810408B13Rik n/a
12 TRCN0000244342 CTGGAGAGAAACCCTACAAAT pLKO_005 1240 CDS 100% 13.200 6.600 Y EG668616 n/a
13 TRCN0000150276 GCCCAGCTAATTTGTGTATTT pLKO.1 3539 3UTR 100% 13.200 6.600 Y SPICE1 n/a
14 TRCN0000136653 GCCTGACCAACATGGTGAAAT pLKO.1 9179 3UTR 100% 13.200 6.600 Y IQCC n/a
15 TRCN0000060503 CCTCTCAAGTAGCTGGGATTA pLKO.1 8702 3UTR 100% 10.800 5.400 Y NCCRP1 n/a
16 TRCN0000155576 CCTCTCAAGTAGCTGGGATTA pLKO.1 8702 3UTR 100% 10.800 5.400 Y KLHL30 n/a
17 TRCN0000165697 CCTCTCAAGTAGCTGGGATTA pLKO.1 8702 3UTR 100% 10.800 5.400 Y SLC48A1 n/a
18 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 9113 3UTR 100% 4.950 2.475 Y CFLAR n/a
19 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 9113 3UTR 100% 4.950 2.475 Y C19orf31 n/a
20 TRCN0000016346 CACTGGAGAGAAACCCTACAA pLKO.1 1658 CDS 100% 4.950 2.475 Y ZNF254 n/a
21 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 2086 3UTR 100% 4.950 2.475 Y ORAI2 n/a
22 TRCN0000183765 CCCTCAATTCTTAACAGACAT pLKO.1 2334 3UTR 100% 4.950 2.475 Y ZNF714 n/a
23 TRCN0000017702 CCCTGGAATATGAAGAGACAT pLKO.1 421 5UTR 100% 4.950 2.475 Y ZNF430 n/a
24 TRCN0000146802 CCTCAAACCTTACTACACATA pLKO.1 1459 CDS 100% 4.950 2.475 Y ZNF714 n/a
25 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 5397 3UTR 100% 4.950 2.475 Y ERAP2 n/a
26 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 5956 3UTR 100% 4.050 2.025 Y P3H4 n/a
27 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 5956 3UTR 100% 4.050 2.025 Y ORAI2 n/a
28 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 5956 3UTR 100% 4.050 2.025 Y P3H4 n/a
29 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 5398 3UTR 100% 13.200 6.600 Y LIAS n/a
30 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 3639 3UTR 100% 5.625 2.813 Y KLHL30 n/a
31 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 2083 3UTR 100% 4.950 2.475 Y LOC339059 n/a
32 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 9111 3UTR 100% 4.950 2.475 Y ERN2 n/a
33 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 9111 3UTR 100% 4.950 2.475 Y P3H4 n/a
34 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 9111 3UTR 100% 4.950 2.475 Y P3H4 n/a
35 TRCN0000158848 GAGAAACCTTACAAATGTGAT pLKO.1 1581 CDS 100% 4.950 2.475 Y ZNF28 n/a
36 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 4108 3UTR 100% 4.950 2.475 Y DCAF11 n/a
37 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 3639 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001319127.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09784 pDONR223 100% 81.3% 81.2% None 0_1ins321;272A>G n/a
2 ccsbBroad304_09784 pLX_304 0% 81.3% 81.2% V5 0_1ins321;272A>G n/a
3 TRCN0000478115 ATTTTTATATATACCACTCGGCCC pLX_317 19.7% 81.3% 81.2% V5 0_1ins321;272A>G n/a
4 ccsbBroadEn_09774 pDONR223 100% 77.7% 66.7% None (many diffs) n/a
5 ccsbBroad304_09774 pLX_304 0% 77.7% 66.7% V5 (many diffs) n/a
6 TRCN0000472815 CCTGACTCCCTTTAAGTGTTCGTG pLX_317 27.4% 77.7% 66.7% V5 (many diffs) n/a
7 ccsbBroadEn_11232 pDONR223 100% 74.9% 65.4% None (many diffs) n/a
8 ccsbBroad304_11232 pLX_304 0% 74.9% 65.4% V5 (many diffs) n/a
9 TRCN0000476048 TACTATCCCAATAGAAACACCTCC pLX_317 21.8% 74.9% 65.4% V5 (many diffs) n/a
10 ccsbBroadEn_15167 pDONR223 53.6% 71% 25.6% None (many diffs) n/a
11 ccsbBroad304_15167 pLX_304 0% 71% 25.6% V5 (not translated due to prior stop codon) (many diffs) n/a
12 ccsbBroadEn_15278 pDONR223 59.5% 68.5% 58.5% None (many diffs) n/a
13 ccsbBroad304_15278 pLX_304 0% 68.5% 58.5% V5 (many diffs) n/a
14 ccsbBroadEn_15273 pDONR223 50.9% 65.7% 59.2% None (many diffs) n/a
15 ccsbBroad304_15273 pLX_304 0% 65.7% 59.2% V5 (many diffs) n/a
16 TRCN0000472761 GCGGTTCAATGTTGTAGTCTTGTG pLX_317 63.8% 23.2% 19.9% V5 (not translated due to frame shift) (many diffs) n/a
17 ccsbBroadEn_02188 pDONR223 100% 61.2% 52.1% None (many diffs) n/a
18 ccsbBroad304_02188 pLX_304 0% 61.2% 52.1% V5 (many diffs) n/a
19 TRCN0000476436 TCCGTGTTGGTCAAGCGACGCGCC pLX_317 12.5% 61.2% 52.1% V5 (many diffs) n/a
20 TRCN0000477054 GAGCCAATTTATTAAACTTAACTA pLX_317 14.1% 51.8% 43.5% V5 (many diffs) n/a
21 ccsbBroadEn_15067 pDONR223 92.3% 51.7% 43.8% None (many diffs) n/a
22 ccsbBroad304_15067 pLX_304 0% 51.7% 43.8% V5 (not translated due to prior stop codon) (many diffs) n/a
23 ccsbBroadEn_07157 pDONR223 100% 50.3% 42.1% None (many diffs) n/a
24 ccsbBroad304_07157 pLX_304 0% 50.3% 42.1% V5 (many diffs) n/a
25 TRCN0000475452 TAAAACTTCAACTTGGTTTCCTTC pLX_317 10.2% 50.3% 42.1% V5 (many diffs) n/a
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