Transcript: Human NM_001076678.3

Homo sapiens zinc finger protein 493 (ZNF493), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
ZNF493 (284443)
Length:
5023
CDS:
106..2430

Additional Resources:

NCBI RefSeq record:
NM_001076678.3
NBCI Gene record:
ZNF493 (284443)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001076678.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000017583 CCGATCTTCAATCCTTAGTAT pLKO.1 1938 CDS 100% 5.625 3.938 N ZNF493 n/a
2 TRCN0000017584 GCTGGGCACAAGCAAATTCAT pLKO.1 2038 CDS 100% 5.625 3.938 N ZNF493 n/a
3 TRCN0000018498 GAAGGGACACAGTACGGTAGT pLKO.1 327 CDS 100% 4.050 2.835 N ZNF493 n/a
4 TRCN0000017586 CCCTTACTAAACATAGGATAA pLKO.1 1529 CDS 100% 10.800 6.480 N ZNF493 n/a
5 TRCN0000018500 CAGCAGGATTTGTATAGGAAA pLKO.1 205 CDS 100% 4.950 2.970 N ZNF493 n/a
6 TRCN0000215374 CTAGAGAGAAACCCTATAAAT pLKO.1 968 CDS 100% 15.000 7.500 Y AI987944 n/a
7 TRCN0000427608 GTGCACAAAGAAGGTTATAAT pLKO_005 505 CDS 100% 15.000 7.500 Y ZNF431 n/a
8 TRCN0000017585 CAGAAGAGAAACCCTACAAAT pLKO.1 1472 CDS 100% 13.200 6.600 Y ZNF493 n/a
9 TRCN0000239639 CAGGAGAGAAACCCTACAAAT pLKO_005 3365 3UTR 100% 13.200 6.600 Y Zfp992 n/a
10 TRCN0000013040 CCCAGTTATATGTTCTCATTT pLKO.1 354 CDS 100% 13.200 6.600 Y ZNF208 n/a
11 TRCN0000428574 CTGAAGAGAAACCCTACAAAT pLKO_005 1556 CDS 100% 13.200 6.600 Y ZNF138 n/a
12 TRCN0000096537 CTGGAGAGAAACCCTACAAAT pLKO.1 1220 CDS 100% 13.200 6.600 Y Zfp934 n/a
13 TRCN0000235358 CTGGAGAGAAACCCTACAAAT pLKO_005 1220 CDS 100% 13.200 6.600 Y 2810408B13Rik n/a
14 TRCN0000244342 CTGGAGAGAAACCCTACAAAT pLKO_005 1220 CDS 100% 13.200 6.600 Y EG668616 n/a
15 TRCN0000019028 GCCTTTAACCAGTCCTCAATT pLKO.1 3403 3UTR 100% 13.200 6.600 Y ZNF92 n/a
16 TRCN0000018502 GATGTTAGAGAACTACAGAAA pLKO.1 228 CDS 100% 4.950 2.475 Y ZNF493 n/a
17 TRCN0000018501 GCCGTTGACATTTAGGGATGT pLKO.1 138 CDS 100% 4.050 2.025 Y ZNF493 n/a
18 TRCN0000284648 ATACAGGAGAGAAACCCTATA pLKO_005 3362 3UTR 100% 10.800 5.400 Y Gm14308 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001076678.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15278 pDONR223 59.5% 65.2% 57.2% None (many diffs) n/a
2 ccsbBroad304_15278 pLX_304 0% 65.2% 57.2% V5 (many diffs) n/a
3 ccsbBroadEn_09784 pDONR223 100% 59.5% 51.5% None (many diffs) n/a
4 ccsbBroad304_09784 pLX_304 0% 59.5% 51.5% V5 (many diffs) n/a
5 TRCN0000478115 ATTTTTATATATACCACTCGGCCC pLX_317 19.7% 59.5% 51.5% V5 (many diffs) n/a
6 ccsbBroadEn_10024 pDONR223 100% 59.3% 48.1% None (many diffs) n/a
7 ccsbBroad304_10024 pLX_304 0% 59.3% 48.1% V5 (many diffs) n/a
8 TRCN0000466950 AAAAATGGGCGCTCTGAGACACAC pLX_317 21.1% 59.3% 48.1% V5 (many diffs) n/a
9 ccsbBroadEn_15167 pDONR223 53.6% 59.1% 26.2% None (many diffs) n/a
10 ccsbBroad304_15167 pLX_304 0% 59.1% 26.2% V5 (not translated due to prior stop codon) (many diffs) n/a
11 ccsbBroadEn_13028 pDONR223 100% 58.2% 49.4% None (many diffs) n/a
12 ccsbBroad304_13028 pLX_304 0% 58.2% 49.4% V5 (many diffs) n/a
13 TRCN0000468257 GTACACCAGACCACTACATGCGAC pLX_317 25.6% 58.2% 49.4% V5 (many diffs) n/a
14 ccsbBroadEn_09774 pDONR223 100% 57.7% 50.1% None (many diffs) n/a
15 ccsbBroad304_09774 pLX_304 0% 57.7% 50.1% V5 (many diffs) n/a
16 TRCN0000472815 CCTGACTCCCTTTAAGTGTTCGTG pLX_317 27.4% 57.7% 50.1% V5 (many diffs) n/a
17 ccsbBroadEn_11550 pDONR223 100% 55.9% 47.2% None (many diffs) n/a
18 ccsbBroad304_11550 pLX_304 0% 55.9% 47.2% V5 (many diffs) n/a
19 ccsbBroadEn_15273 pDONR223 50.9% 55.2% 48.3% None (many diffs) n/a
20 ccsbBroad304_15273 pLX_304 0% 55.2% 48.3% V5 (many diffs) n/a
21 TRCN0000472761 GCGGTTCAATGTTGTAGTCTTGTG pLX_317 63.8% 26.2% 22% V5 (not translated due to frame shift) (many diffs) n/a
22 ccsbBroadEn_10299 pDONR223 100% 11.7% 9.6% None (many diffs) n/a
23 ccsbBroad304_10299 pLX_304 0% 11.7% 9.6% V5 (many diffs) n/a
24 TRCN0000470427 ATGCTGATTAAATGGTCGTCTGCC pLX_317 95.5% 11.7% 9.6% V5 (many diffs) n/a
25 ccsbBroadEn_11384 pDONR223 100% 10.2% 8.9% None (many diffs) n/a
26 ccsbBroad304_11384 pLX_304 0% 10.2% 8.9% V5 (many diffs) n/a
27 TRCN0000470576 TACATACAGACCTACACGTAGACC pLX_317 100% 10.2% 8.9% V5 (many diffs) n/a
28 ccsbBroadEn_15729 pDONR223 0% 9.3% 8.1% None (many diffs) n/a
29 ccsbBroad304_15729 pLX_304 0% 9.3% 8.1% V5 (many diffs) n/a
30 TRCN0000470492 GCCGACTTGCTCCATGATGCAGCT pLX_317 100% 9.3% 8.1% V5 (many diffs) n/a
31 ccsbBroadEn_13746 pDONR223 100% 8.6% 7.6% None (many diffs) n/a
32 ccsbBroad304_13746 pLX_304 0% 8.6% 7.6% V5 (many diffs) n/a
33 TRCN0000475669 ATTCGCAGCGAGTTTGTCCGCCGC pLX_317 100% 8.6% 7.6% V5 (many diffs) n/a
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