Transcript: Human NM_173531.4

Homo sapiens zinc finger protein 100 (ZNF100), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
ZNF100 (163227)
Length:
5691
CDS:
151..1779

Additional Resources:

NCBI RefSeq record:
NM_173531.4
NBCI Gene record:
ZNF100 (163227)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_173531.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000235915 GTTAGGCATTCAGAGTAATAT pLKO_005 3029 3UTR 100% 15.000 21.000 N ZNF100 n/a
2 TRCN0000020830 GCACAAAGAACATGATAACAA pLKO.1 621 CDS 100% 5.625 7.875 N ZNF100 n/a
3 TRCN0000235914 CAGTCCCTAAGCCTTATTAAA pLKO_005 1720 CDS 100% 15.000 10.500 N ZNF100 n/a
4 TRCN0000020831 CCAGTCCCTAAGCCTTATTAA pLKO.1 1719 CDS 100% 15.000 10.500 N ZNF100 n/a
5 TRCN0000235913 AGTGCACAAAGAACATGATAA pLKO_005 618 CDS 100% 13.200 9.240 N ZNF100 n/a
6 TRCN0000235917 GAGTTTGTGTGTATGAATTTG pLKO_005 4142 3UTR 100% 13.200 9.240 N ZNF100 n/a
7 TRCN0000020829 CCCTAAGCCTTATTAAACAAA pLKO.1 1724 CDS 100% 5.625 3.938 N ZNF100 n/a
8 TRCN0000020833 CCTCACAACTAACTGCACATA pLKO.1 1556 CDS 100% 4.950 3.465 N ZNF100 n/a
9 TRCN0000235916 AGTCTTCTGGTGCAGTCTTAT pLKO_005 217 CDS 100% 13.200 7.920 N ZNF100 n/a
10 TRCN0000020832 CCCTGGAATATAAAGAGACAT pLKO.1 430 CDS 100% 4.950 2.970 N ZNF100 n/a
11 TRCN0000236731 ACCTTACTACACATAAGATAA pLKO_005 974 CDS 100% 13.200 6.600 Y ZNF98 n/a
12 TRCN0000013040 CCCAGTTATATGTTCTCATTT pLKO.1 468 CDS 100% 13.200 6.600 Y ZNF208 n/a
13 TRCN0000236730 CCCTTACTACACATAAGATAA pLKO_005 1226 CDS 100% 13.200 6.600 Y ZNF98 n/a
14 TRCN0000096537 CTGGAGAGAAACCCTACAAAT pLKO.1 917 CDS 100% 13.200 6.600 Y Zfp934 n/a
15 TRCN0000235358 CTGGAGAGAAACCCTACAAAT pLKO_005 917 CDS 100% 13.200 6.600 Y 2810408B13Rik n/a
16 TRCN0000244342 CTGGAGAGAAACCCTACAAAT pLKO_005 917 CDS 100% 13.200 6.600 Y EG668616 n/a
17 TRCN0000421971 TTACACCTAACTCAACATAAA pLKO_005 802 CDS 100% 13.200 6.600 Y ZNF430 n/a
18 TRCN0000165774 CCTCCTAAGTAGCTGGGATTA pLKO.1 3513 3UTR 100% 10.800 5.400 Y SNX29P1 n/a
19 TRCN0000018499 CTGGTCTTCTTGGCAGGTATT pLKO.1 361 CDS 100% 10.800 5.400 Y ZNF493 n/a
20 TRCN0000419715 TGGTCTTCTTGGCAGGTATTG pLKO_005 362 CDS 100% 10.800 5.400 Y ZNF430 n/a
21 TRCN0000149130 GCTGGAGAGAAACCTTACAAA pLKO.1 1252 CDS 100% 5.625 2.813 Y ZNF761 n/a
22 TRCN0000018502 GATGTTAGAGAACTACAGAAA pLKO.1 339 CDS 100% 4.950 2.475 Y ZNF493 n/a
23 TRCN0000158848 GAGAAACCTTACAAATGTGAT pLKO.1 1258 CDS 100% 4.950 2.475 Y ZNF28 n/a
24 TRCN0000149073 GCAAAGCCTTTAACCAGTCTT pLKO.1 1202 CDS 100% 4.950 2.475 Y ZNF714 n/a
25 TRCN0000149336 GCAAAGGCTTTAACTGGTCTT pLKO.1 1370 CDS 100% 4.050 2.025 Y ZNF714 n/a
26 TRCN0000019028 GCCTTTAACCAGTCCTCAATT pLKO.1 1207 CDS 100% 13.200 6.600 Y ZNF92 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_173531.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15273 pDONR223 50.9% 99.8% 99.8% None 1166C>T;1599C>T n/a
2 ccsbBroad304_15273 pLX_304 0% 99.8% 99.8% V5 1166C>T;1599C>T n/a
3 TRCN0000472761 GCGGTTCAATGTTGTAGTCTTGTG pLX_317 63.8% 48.4% 48.5% V5 (not translated due to frame shift) 789_1626del n/a
4 ccsbBroadEn_15167 pDONR223 53.6% 85.7% 41.5% None (many diffs) n/a
5 ccsbBroad304_15167 pLX_304 0% 85.7% 41.5% V5 (not translated due to prior stop codon) (many diffs) n/a
6 ccsbBroadEn_09784 pDONR223 100% 81.8% 73.1% None (many diffs) n/a
7 ccsbBroad304_09784 pLX_304 0% 81.8% 73.1% V5 (many diffs) n/a
8 TRCN0000478115 ATTTTTATATATACCACTCGGCCC pLX_317 19.7% 81.8% 73.1% V5 (many diffs) n/a
9 ccsbBroadEn_15278 pDONR223 59.5% 70% 60.3% None (many diffs) n/a
10 ccsbBroad304_15278 pLX_304 0% 70% 60.3% V5 (many diffs) n/a
11 ccsbBroadEn_09655 pDONR223 100% 69.1% 58.4% None (many diffs) n/a
12 ccsbBroad304_09655 pLX_304 0% 69.1% 58.4% V5 (many diffs) n/a
13 TRCN0000468487 GAACACCAGATCGGTGGCCCTCAA pLX_317 20.1% 69.1% 58.4% V5 (many diffs) n/a
14 ccsbBroadEn_02188 pDONR223 100% 65.1% 57.1% None (many diffs) n/a
15 ccsbBroad304_02188 pLX_304 0% 65.1% 57.1% V5 (many diffs) n/a
16 TRCN0000476436 TCCGTGTTGGTCAAGCGACGCGCC pLX_317 12.5% 65.1% 57.1% V5 (many diffs) n/a
17 ccsbBroadEn_09774 pDONR223 100% 61.1% 51.6% None (many diffs) n/a
18 ccsbBroad304_09774 pLX_304 0% 61.1% 51.6% V5 (many diffs) n/a
19 TRCN0000472815 CCTGACTCCCTTTAAGTGTTCGTG pLX_317 27.4% 61.1% 51.6% V5 (many diffs) n/a
20 TRCN0000477054 GAGCCAATTTATTAAACTTAACTA pLX_317 14.1% 42.6% 35% V5 (many diffs) n/a
21 ccsbBroadEn_15067 pDONR223 92.3% 42.6% 35.2% None (many diffs) n/a
22 ccsbBroad304_15067 pLX_304 0% 42.6% 35.2% V5 (not translated due to prior stop codon) (many diffs) n/a
23 ccsbBroadEn_11235 pDONR223 100% 27.2% 21.5% None (many diffs) n/a
24 ccsbBroad304_11235 pLX_304 0% 27.2% 21.5% V5 (many diffs) n/a
25 TRCN0000481650 TTTATCAAAGGAATCCCCAATTTT pLX_317 100% 27.2% 21.5% V5 (many diffs) n/a
26 ccsbBroadEn_15729 pDONR223 0% 13.3% 11.5% None (many diffs) n/a
27 ccsbBroad304_15729 pLX_304 0% 13.3% 11.5% V5 (many diffs) n/a
28 TRCN0000470492 GCCGACTTGCTCCATGATGCAGCT pLX_317 100% 13.3% 11.5% V5 (many diffs) n/a
29 ccsbBroadEn_13746 pDONR223 100% 12.2% 10.8% None (many diffs) n/a
30 ccsbBroad304_13746 pLX_304 0% 12.2% 10.8% V5 (many diffs) n/a
31 TRCN0000475669 ATTCGCAGCGAGTTTGTCCGCCGC pLX_317 100% 12.2% 10.8% V5 (many diffs) n/a
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