Transcript: Human XM_024451653.1

PREDICTED: Homo sapiens zinc finger protein 723 (ZNF723), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZNF723 (646864)
Length:
3315
CDS:
1197..2840

Additional Resources:

NCBI RefSeq record:
XM_024451653.1
NBCI Gene record:
ZNF723 (646864)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024451653.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000236730 CCCTTACTACACATAAGATAA pLKO_005 2428 CDS 100% 13.200 6.600 Y ZNF98 n/a
2 TRCN0000096537 CTGGAGAGAAACCCTACAAAT pLKO.1 1867 CDS 100% 13.200 6.600 Y Zfp934 n/a
3 TRCN0000235358 CTGGAGAGAAACCCTACAAAT pLKO_005 1867 CDS 100% 13.200 6.600 Y 2810408B13Rik n/a
4 TRCN0000244342 CTGGAGAGAAACCCTACAAAT pLKO_005 1867 CDS 100% 13.200 6.600 Y EG668616 n/a
5 TRCN0000107875 CACTTGATTGTAGGTAAGATA pLKO.1 2763 CDS 100% 5.625 2.813 Y ZNF254 n/a
6 TRCN0000016346 CACTGGAGAGAAACCCTACAA pLKO.1 2033 CDS 100% 4.950 2.475 Y ZNF254 n/a
7 TRCN0000018502 GATGTTAGAGAACTACAGAAA pLKO.1 1292 CDS 100% 4.950 2.475 Y ZNF493 n/a
8 TRCN0000017702 CCCTGGAATATGAAGAGACAT pLKO.1 1380 CDS 100% 4.950 2.475 Y ZNF430 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024451653.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10024 pDONR223 100% 79.7% 68.8% None (many diffs) n/a
2 ccsbBroad304_10024 pLX_304 0% 79.7% 68.8% V5 (many diffs) n/a
3 TRCN0000466950 AAAAATGGGCGCTCTGAGACACAC pLX_317 21.1% 79.7% 68.8% V5 (many diffs) n/a
4 ccsbBroadEn_15278 pDONR223 59.5% 79.2% 69.4% None (many diffs) n/a
5 ccsbBroad304_15278 pLX_304 0% 79.2% 69.4% V5 (many diffs) n/a
6 ccsbBroadEn_15167 pDONR223 53.6% 78.8% 34.2% None (many diffs) n/a
7 ccsbBroad304_15167 pLX_304 0% 78.8% 34.2% V5 (not translated due to prior stop codon) (many diffs) n/a
8 ccsbBroadEn_11550 pDONR223 100% 76.8% 65.6% None (many diffs) n/a
9 ccsbBroad304_11550 pLX_304 0% 76.8% 65.6% V5 (many diffs) n/a
10 ccsbBroadEn_08635 pDONR223 100% 76% 62.5% None (many diffs) n/a
11 ccsbBroad304_08635 pLX_304 0% 76% 62.5% V5 (many diffs) n/a
12 TRCN0000469248 TACCCGCTTGGCTTTAAAAACCAA pLX_317 37.7% 60.7% 48.2% V5 (many diffs) n/a
13 ccsbBroadEn_15273 pDONR223 50.9% 73.9% 63% None (many diffs) n/a
14 ccsbBroad304_15273 pLX_304 0% 73.9% 63% V5 (many diffs) n/a
15 TRCN0000472761 GCGGTTCAATGTTGTAGTCTTGTG pLX_317 63.8% 34.3% 28.8% V5 (not translated due to frame shift) (many diffs) n/a
16 ccsbBroadEn_11232 pDONR223 100% 68% 58.5% None (many diffs) n/a
17 ccsbBroad304_11232 pLX_304 0% 68% 58.5% V5 (many diffs) n/a
18 TRCN0000476048 TACTATCCCAATAGAAACACCTCC pLX_317 21.8% 68% 58.5% V5 (many diffs) n/a
19 ccsbBroadEn_07157 pDONR223 100% 58.5% 49.8% None (many diffs) n/a
20 ccsbBroad304_07157 pLX_304 0% 58.5% 49.8% V5 (many diffs) n/a
21 TRCN0000475452 TAAAACTTCAACTTGGTTTCCTTC pLX_317 10.2% 58.5% 49.8% V5 (many diffs) n/a
22 ccsbBroadEn_11235 pDONR223 100% 27.2% 20.1% None (many diffs) n/a
23 ccsbBroad304_11235 pLX_304 0% 27.2% 20.1% V5 (many diffs) n/a
24 TRCN0000481650 TTTATCAAAGGAATCCCCAATTTT pLX_317 100% 27.2% 20.1% V5 (many diffs) n/a
25 ccsbBroadEn_15729 pDONR223 0% 13.2% 12.1% None (many diffs) n/a
26 ccsbBroad304_15729 pLX_304 0% 13.2% 12.1% V5 (many diffs) n/a
27 TRCN0000470492 GCCGACTTGCTCCATGATGCAGCT pLX_317 100% 13.2% 12.1% V5 (many diffs) n/a
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