Construct: ORF ccsbBroadEn_01287
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016790.1_s300c1, BRDN0000391274
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAPK8 (5599)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001278547.1 | 100% | 100% | |
| 2 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323322.2 | 100% | 100% | |
| 3 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323323.2 | 100% | 100% | |
| 4 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323330.2 | 100% | 100% | |
| 5 | human | 5599 | MAPK8 | mitogen-activated protein k... | XM_024448079.1 | 100% | 100% | |
| 6 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323328.2 | 97.6% | 98.1% | (many diffs) |
| 7 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323329.2 | 97.6% | 98.1% | (many diffs) |
| 8 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323331.2 | 97.6% | 98.1% | (many diffs) |
| 9 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_139049.4 | 97.6% | 98.1% | (many diffs) |
| 10 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323321.2 | 89.1% | 88.7% | 1137_1141delAGCAC;1152_1153ins134 |
| 11 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323325.2 | 89.1% | 88.7% | 1137_1141delAGCAC;1152_1153ins134 |
| 12 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_139046.4 | 89.1% | 88.7% | 1137_1141delAGCAC;1152_1153ins134 |
| 13 | human | 5599 | MAPK8 | mitogen-activated protein k... | XM_024448080.1 | 89.1% | 88.7% | 1137_1141delAGCAC;1152_1153ins134 |
| 14 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323302.2 | 86.9% | 86.8% | (many diffs) |
| 15 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323324.2 | 86.9% | 86.8% | (many diffs) |
| 16 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323326.2 | 86.9% | 86.8% | (many diffs) |
| 17 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323327.2 | 86.9% | 86.8% | (many diffs) |
| 18 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323320.2 | 82.2% | 82.2% | 615_616ins228 |
| 19 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008427.2 | 77.4% | 90.8% | (many diffs) |
| 20 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008428.2 | 77.4% | 90.8% | (many diffs) |
| 21 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008433.2 | 72.7% | 85.4% | (many diffs) |
| 22 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001278548.1 | 71.4% | 70.9% | 615_616ins228;909_913delAGCAC;924_925ins134 |
| 23 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_005263131.3 | 71.1% | 83.4% | (many diffs) |
| 24 | human | 5602 | MAPK10 | mitogen-activated protein k... | NM_001363657.2 | 70.4% | 82.4% | (many diffs) |
| 25 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008422.2 | 66.8% | 78.4% | (many diffs) |
| 26 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008441.2 | 65.7% | 77% | (many diffs) |
| 27 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_006714269.3 | 64.7% | 75.6% | (many diffs) |
| 28 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008449.2 | 58.2% | 67.9% | (many diffs) |
| 29 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008450.2 | 58.2% | 67.9% | (many diffs) |
| 30 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454148.1 | 58.2% | 67.9% | (many diffs) |
| 31 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008453.2 | 36% | 42.3% | (many diffs) |
| 32 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008454.2 | 35.4% | 42.1% | (many diffs) |
| 33 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454149.1 | 35.4% | 42.1% | (many diffs) |
| 34 | human | 5599 | MAPK8 | mitogen-activated protein k... | NR_136585.2 | 21.9% | (many diffs) | |
| 35 | human | 5599 | MAPK8 | mitogen-activated protein k... | NR_136584.2 | 21.5% | (many diffs) | |
| 36 | human | 5599 | MAPK8 | mitogen-activated protein k... | NR_136583.2 | 21.5% | (many diffs) | |
| 37 | mouse | 26419 | Mapk8 | mitogen-activated protein k... | NM_001310453.1 | 94% | 99% | (many diffs) |
| 38 | mouse | 26419 | Mapk8 | mitogen-activated protein k... | NM_001310454.1 | 91.8% | 97.1% | (many diffs) |
| 39 | mouse | 26419 | Mapk8 | mitogen-activated protein k... | NM_001310452.1 | 83.9% | 88.7% | (many diffs) |
| 40 | mouse | 26419 | Mapk8 | mitogen-activated protein k... | XM_006519032.3 | 83.9% | 88.7% | (many diffs) |
| 41 | mouse | 26419 | Mapk8 | mitogen-activated protein k... | NM_016700.4 | 81.8% | 86.8% | (many diffs) |
| 42 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_017320889.2 | 75.4% | 90.6% | (many diffs) |
| 43 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_017320886.2 | 69.3% | 83.2% | (many diffs) |
| 44 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_030254519.1 | 68.7% | 82.2% | (many diffs) |
| 45 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_006534950.4 | 63.1% | 75.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1350
- ORF length:
- 1281
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GAGCAGAAGC AAGCGTGACA ACAATTTTTA TAGTGTAGAG ATTGGAGATT 121 CTACATTCAC AGTCCTGAAA CGATATCAGA ATTTAAAACC TATAGGCTCA GGAGCTCAAG 181 GAATAGTATG CGCAGCTTAT GATGCCATTC TTGAAAGAAA TGTTGCAATC AAGAAGCTAA 241 GCCGACCATT TCAGAATCAG ACTCATGCCA AGCGGGCCTA CAGAGAGCTA GTTCTTATGA 301 AATGTGTTAA TCACAAAAAT ATAATTGGCC TTTTGAATGT TTTCACACCA CAGAAATCCC 361 TAGAAGAATT TCAAGATGTT TACATAGTCA TGGAGCTCAT GGATGCAAAT CTTTGCCAAG 421 TGATTCAGAT GGAGCTAGAT CATGAAAGAA TGTCCTACCT TCTCTATCAG ATGCTGTGTG 481 GAATCAAGCA CCTTCATTCT GCTGGAATTA TTCATCGGGA CTTAAAGCCC AGTAATATAG 541 TAGTAAAATC TGATTGCACT TTGAAGATTC TTGACTTCGG TCTGGCCAGG ACTGCAGGAA 601 CGAGTTTTAT GATGACGCCT TATGTAGTGA CTCGCTACTA CAGAGCACCC GAGGTCATCC 661 TTGGCATGGG CTACAAGGAA AACGTTGACA TTTGGTCAGT TGGGTGCATC ATGGGAGAAA 721 TGATCAAAGG TGGTGTTTTG TTCCCAGGTA CAGATCATAT TGATCAGTGG AATAAAGTTA 781 TTGAACAGCT TGGAACACCA TGTCCTGAAT TCATGAAGAA ACTGCAACCA ACAGTAAGGA 841 CTTACGTTGA AAACAGACCT AAATATGCTG GATATAGCTT TGAGAAACTC TTCCCTGATG 901 TCCTTTTCCC AGCTGACTCA GAACACAACA AACTTAAAGC CAGTCAGGCA AGGGATTTGT 961 TATCCAAAAT GCTGGTAATA GATGCATCTA AAAGGATCTC TGTAGATGAA GCTCTCCAAC 1021 ACCCGTACAT CAATGTCTGG TATGATCCTT CTGAAGCAGA AGCTCCACCA CCAAAGATCC 1081 CTGACAAGCA GTTAGATGAA AGGGAACACA CAATAGAAGA GTGGAAAGAA TTGATATATA 1141 AGGAAGTTAT GGACTTGGAG GAGAGAACCA AGAATGGAGT TATACGGGGG CAGCCCTCTC 1201 CTTTAGGTGC AGCAGTGATC AATGGCTCTC AGCATCCATC ATCATCGTCG TCTGTCAATG 1261 ATGTGTCTTC AATGTCAACA GATCCGACTT TGGCCTCTGA TACAGACAGC AGTCTAGAAG 1321 CAGCAGCTGG GCCTCTGGGC TGCTGTAGAT TGCCAACTTT CTTGTACAAA GTtggcatta 1381 taagaaagca ttgcttatca atttgttgca acgaac