Transcript: Human XM_017008422.2

PREDICTED: Homo sapiens mitogen-activated protein kinase 10 (MAPK10), transcript variant X12, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MAPK10 (5602)
Length:
6258
CDS:
7..1329

Additional Resources:

NCBI RefSeq record:
XM_017008422.2
NBCI Gene record:
MAPK10 (5602)

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017008422.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000194704 CATACAGCACTACTTACTTAG pLKO.1 1792 3UTR 100% 10.800 15.120 N MAPK10 n/a
2 TRCN0000433905 GTCAAGTCTGATTGCACATTG pLKO_005 595 CDS 100% 10.800 15.120 N MAPK10 n/a
3 TRCN0000196303 GAATTAGACCATGAGCGAATG pLKO.1 484 CDS 100% 6.000 8.400 N MAPK10 n/a
4 TRCN0000001021 CGACGCCTTACAGCATCCCTA pLKO.1 987 CDS 100% 0.880 1.232 N MAPK10 n/a
5 TRCN0000432277 GCCTAGTCAGATGGATGTAGA pLKO_005 1747 3UTR 100% 4.950 3.960 N MAPK10 n/a
6 TRCN0000001020 GCCATTAAGAAGCTCAGCAGA pLKO.1 277 CDS 100% 2.640 2.112 N MAPK10 n/a
7 TRCN0000001938 GCCATTAAGAAGCTCAGCAGA pLKO.1 277 CDS 100% 2.640 2.112 N MAPK10 n/a
8 TRCN0000195168 CAAGTGGATGTGTCATATATT pLKO.1 85 CDS 100% 15.000 10.500 N MAPK10 n/a
9 TRCN0000433496 GAATTATTCACAGGGATTTAA pLKO_005 557 CDS 100% 15.000 10.500 N MAPK10 n/a
10 TRCN0000417287 ATGAAGTGTGTGAACCATAAA pLKO_005 349 CDS 100% 13.200 9.240 N MAPK10 n/a
11 TRCN0000194965 CCAAATGTTGTGTGGCATTAA pLKO.1 519 CDS 100% 13.200 9.240 N MAPK10 n/a
12 TRCN0000194979 CCATTTCATGTGATCTATTAC pLKO.1 1876 3UTR 100% 13.200 9.240 N MAPK10 n/a
13 TRCN0000196370 GACAGAAATGTGGCCATTAAG pLKO.1 265 CDS 100% 13.200 9.240 N MAPK10 n/a
14 TRCN0000424750 GTATTGCAGCTAAGCTCAAAT pLKO_005 1507 3UTR 100% 13.200 9.240 N MAPK10 n/a
15 TRCN0000417185 GCCAGGGACTTGTTGTCAAAG pLKO_005 928 CDS 100% 10.800 7.560 N MAPK10 n/a
16 TRCN0000422541 TGACCAGTGGAATAAGGTAAT pLKO_005 741 CDS 100% 10.800 7.560 N MAPK10 n/a
17 TRCN0000001937 CCTTACAGCATCCCTACATCA pLKO.1 992 CDS 100% 4.950 3.465 N MAPK10 n/a
18 TRCN0000001018 GTAAGAAACTATGTGGAGAAT pLKO.1 814 CDS 100% 4.950 3.465 N MAPK10 n/a
19 TRCN0000001939 GTAAGAAACTATGTGGAGAAT pLKO.1 814 CDS 100% 4.950 3.465 N MAPK10 n/a
20 TRCN0000001019 GCCGCGTATGATGCTGTCCTT pLKO.1 244 CDS 100% 0.880 0.616 N MAPK10 n/a
21 TRCN0000001941 CAATAAACTCAAAGCCAGCCA pLKO.1 906 CDS 100% 0.660 0.462 N MAPK10 n/a
22 TRCN0000001017 CCAATGATGCTTACTACAGAA pLKO.1 1567 3UTR 100% 4.950 2.970 N MAPK10 n/a
23 TRCN0000001940 CCAATGATGCTTACTACAGAA pLKO.1 1567 3UTR 100% 4.950 2.970 N MAPK10 n/a
24 TRCN0000166364 CACACACACACACACACACAA pLKO.1 4213 3UTR 100% 4.950 2.475 Y KAAG1 n/a
25 TRCN0000162548 CACACACACACACACAAATAT pLKO.1 1398 3UTR 100% 15.000 7.500 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017008422.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000487812 TGCAGATTATTTTACTCCTCGACG pLX_317 20.5% 94.4% 84.4% V5 (not translated due to prior stop codon) 730_731ins72;1177_1178insCAGCA n/a
2 TRCN0000488648 ACCAGGGTGAGTGAATCTAAGAAG pLX_317 20.3% 94.4% 84.4% V5 (not translated due to prior stop codon) 730_731ins72;1177_1178insCAGCA;1320_1321insG n/a
3 TRCN0000492117 CTAAGCTAACTACGGCGGTACGCC pLX_317 31.1% 85% 84.4% V5 (many diffs) n/a
4 TRCN0000491457 CCGTCCACGCTTTTACTCGGCAGT pLX_317 24.4% 85% 84.4% V5 (not translated due to prior stop codon) (many diffs) n/a
5 ccsbBroadEn_01287 pDONR223 100% 66.8% 78.4% None (many diffs) n/a
6 ccsbBroad304_01287 pLX_304 52.6% 66.8% 78.4% V5 (many diffs) n/a
7 ccsbBroadEn_11057 pDONR223 100% 63.5% 63.3% None 1_435del;730_731ins72 n/a
8 ccsbBroad304_11057 pLX_304 0% 63.5% 63.3% V5 1_435del;730_731ins72 n/a
9 TRCN0000468220 CCCGCTATCGAACCGGGCATATAA pLX_317 45% 63.5% 63.3% V5 1_435del;730_731ins72 n/a
10 ccsbBroadEn_14805 pDONR223 0% 63.5% 63.3% None 1_435del;730_731ins72 n/a
11 ccsbBroad304_14805 pLX_304 0% 63.5% 63.3% V5 1_435del;730_731ins72 n/a
12 TRCN0000466133 TCATAAGCAAACAGGTTCCCACAG pLX_317 29.1% 63.5% 63.3% V5 1_435del;730_731ins72 n/a
13 ccsbBroadEn_11058 pDONR223 100% 63.5% 63.3% None 1_435del;730_731ins72 n/a
14 ccsbBroad304_11058 pLX_304 0% 63.5% 63.3% V5 1_435del;730_731ins72 n/a
15 TRCN0000475774 GTGAGTTGCCAATCATGCAATTTA pLX_317 29% 63.5% 63.3% V5 1_435del;730_731ins72 n/a
16 TRCN0000489471 GTGAGACGATACCTATTGCCACGT pLX_317 32.6% 60.5% 70.4% V5 (not translated due to prior stop codon) (many diffs) n/a
17 TRCN0000492156 AACAGCCTCTTGTTACGAGGTCTG pLX_317 19.2% 60.5% 70.4% V5 (not translated due to prior stop codon) (many diffs) n/a
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