Transcript: Mouse NM_001310686.2

Mus musculus mitogen-activated protein kinase 10 (Mapk10), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-09-15
Taxon:
Mus musculus (mouse)
Gene:
Mapk10 (26414)
Length:
6071
CDS:
148..1302

Additional Resources:

NCBI RefSeq record:
NM_001310686.2
NBCI Gene record:
Mapk10 (26414)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001310686.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000360388 GCGGATTCTGAGCACAATAAA pLKO_005 991 CDS 100% 15.000 21.000 N Mapk10 n/a
2 TRCN0000360389 GTATGTGGTGACGCGATATTA pLKO_005 699 CDS 100% 15.000 21.000 N Mapk10 n/a
3 TRCN0000194979 CCATTTCATGTGATCTATTAC pLKO.1 2053 3UTR 100% 13.200 18.480 N MAPK10 n/a
4 TRCN0000012633 GCGATAGAACACAGCACACAT pLKO.1 1477 3UTR 100% 4.950 6.930 N Mapk10 n/a
5 TRCN0000201207 GTTGGAACCAAGTCAACGTAT pLKO.1 5979 3UTR 100% 4.950 6.930 N Mapk10 n/a
6 TRCN0000360315 CAATAGAGAGATCCAACATAA pLKO_005 1634 3UTR 100% 13.200 10.560 N Mapk10 n/a
7 TRCN0000417287 ATGAAGTGTGTGAACCATAAA pLKO_005 376 CDS 100% 13.200 9.240 N MAPK10 n/a
8 TRCN0000191064 CCAAGTCAACGTATTGTAATT pLKO.1 5986 3UTR 100% 13.200 9.240 N Mapk10 n/a
9 TRCN0000012634 CGAAGAATGGAAAGAACTTAT pLKO.1 1194 CDS 100% 13.200 9.240 N Mapk10 n/a
10 TRCN0000216662 CTCAACCTTCACCGTTCTTAA pLKO.1 198 CDS 100% 13.200 9.240 N Mapk10 n/a
11 TRCN0000196370 GACAGAAATGTGGCCATTAAG pLKO.1 292 CDS 100% 13.200 9.240 N MAPK10 n/a
12 TRCN0000012636 GTGTCATCTATTGCCAAACAT pLKO.1 121 5UTR 100% 5.625 3.938 N Mapk10 n/a
13 TRCN0000012637 CAAGTCTGATTGCACACTGAA pLKO.1 624 CDS 100% 4.950 3.465 N Mapk10 n/a
14 TRCN0000012635 CCAAGATGTCTACTTAGTGAT pLKO.1 450 CDS 100% 4.950 3.465 N Mapk10 n/a
15 TRCN0000432277 GCCTAGTCAGATGGATGTAGA pLKO_005 1933 3UTR 100% 4.950 3.465 N MAPK10 n/a
16 TRCN0000191558 GAATCATTAAAGCTGAAGGAA pLKO.1 6019 3UTR 100% 3.000 2.100 N Mapk10 n/a
17 TRCN0000001020 GCCATTAAGAAGCTCAGCAGA pLKO.1 304 CDS 100% 2.640 1.848 N MAPK10 n/a
18 TRCN0000001938 GCCATTAAGAAGCTCAGCAGA pLKO.1 304 CDS 100% 2.640 1.848 N MAPK10 n/a
19 TRCN0000166364 CACACACACACACACACACAA pLKO.1 3468 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001310686.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491457 CCGTCCACGCTTTTACTCGGCAGT pLX_317 24.4% 82.5% 90.9% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000492117 CTAAGCTAACTACGGCGGTACGCC pLX_317 31.1% 82.4% 90.7% V5 (many diffs) n/a
3 TRCN0000487812 TGCAGATTATTTTACTCCTCGACG pLX_317 20.5% 74.8% 90.9% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000488648 ACCAGGGTGAGTGAATCTAAGAAG pLX_317 20.3% 74.8% 90.9% V5 (not translated due to prior stop codon) (many diffs) n/a
5 ccsbBroadEn_11057 pDONR223 100% 57.9% 63.8% None (many diffs) n/a
6 ccsbBroad304_11057 pLX_304 0% 57.9% 63.8% V5 (many diffs) n/a
7 TRCN0000468220 CCCGCTATCGAACCGGGCATATAA pLX_317 45% 57.9% 63.8% V5 (many diffs) n/a
8 ccsbBroadEn_14805 pDONR223 0% 57.9% 63.8% None (many diffs) n/a
9 ccsbBroad304_14805 pLX_304 0% 57.9% 63.8% V5 (many diffs) n/a
10 TRCN0000466133 TCATAAGCAAACAGGTTCCCACAG pLX_317 29.1% 57.9% 63.8% V5 (many diffs) n/a
11 ccsbBroadEn_11058 pDONR223 100% 56.2% 61.9% None (many diffs) n/a
12 ccsbBroad304_11058 pLX_304 0% 56.2% 61.9% V5 (many diffs) n/a
13 TRCN0000475774 GTGAGTTGCCAATCATGCAATTTA pLX_317 29% 56.2% 61.9% V5 (many diffs) n/a
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