Construct: ORF TRCN0000491457
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020400.2_s317c1
- DNA Barcode:
- CCGTCCACGCTTTTACTCGGCAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAPK10 (5602)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491457
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 5602 | MAPK10 | mitogen-activated protein k... | NM_002753.5 | 99.9% | 100% | 960G>A |
2 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_005263135.4 | 99.9% | 100% | 960G>A |
3 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008429.2 | 99.9% | 100% | 960G>A |
4 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008430.2 | 99.9% | 100% | 960G>A |
5 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008432.2 | 99.9% | 100% | 960G>A |
6 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454144.1 | 99.9% | 100% | 960G>A |
7 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454145.1 | 99.9% | 100% | 960G>A |
8 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454146.1 | 99.9% | 100% | 960G>A |
9 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454147.1 | 99.9% | 100% | 960G>A |
10 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_006714269.3 | 97.5% | 98.1% | (many diffs) |
11 | human | 5602 | MAPK10 | mitogen-activated protein k... | NM_001351625.2 | 90.9% | 90.9% | 0_1ins114;846G>A |
12 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008434.2 | 90.9% | 90.9% | 0_1ins114;846G>A |
13 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008435.2 | 90.9% | 90.9% | 0_1ins114;846G>A |
14 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008436.2 | 90.9% | 90.9% | 0_1ins114;846G>A |
15 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008437.2 | 90.9% | 90.9% | 0_1ins114;846G>A |
16 | human | 5602 | MAPK10 | mitogen-activated protein k... | NM_138982.4 | 90.1% | 89.8% | 960G>A;1249_1250insCAGCA;1262_1392del |
17 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_005263129.3 | 90.1% | 89.8% | 960G>A;1249_1250insCAGCA;1262_1392del |
18 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_005263130.3 | 90.1% | 89.8% | 960G>A;1249_1250insCAGCA;1262_1392del |
19 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_011532117.3 | 90.1% | 89.8% | 960G>A;1249_1250insCAGCA;1262_1392del |
20 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_011532118.3 | 90.1% | 89.8% | 960G>A;1249_1250insCAGCA;1262_1392del |
21 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008420.2 | 90.1% | 89.8% | 960G>A;1249_1250insCAGCA;1262_1392del |
22 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454139.1 | 90.1% | 89.8% | 960G>A;1249_1250insCAGCA;1262_1392del |
23 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454140.1 | 90.1% | 89.8% | 960G>A;1249_1250insCAGCA;1262_1392del |
24 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454141.1 | 90.1% | 89.8% | 960G>A;1249_1250insCAGCA;1262_1392del |
25 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454142.1 | 90.1% | 89.8% | 960G>A;1249_1250insCAGCA;1262_1392del |
26 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454143.1 | 90.1% | 89.8% | 960G>A;1249_1250insCAGCA;1262_1392del |
27 | human | 5602 | MAPK10 | mitogen-activated protein k... | NM_001363657.2 | 88.6% | 89% | (many diffs) |
28 | human | 5602 | MAPK10 | mitogen-activated protein k... | NM_001318067.1 | 88.1% | 88.1% | (many diffs) |
29 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_005263131.3 | 88.1% | 88.1% | (many diffs) |
30 | human | 5602 | MAPK10 | mitogen-activated protein k... | NM_001318069.2 | 87.5% | 89.8% | 960G>A;1249_1250insCAGCA;1262_1434del |
31 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008441.2 | 85.2% | 85% | 0_1ins114;616_617ins72;774G>A |
32 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008422.2 | 85% | 84.4% | (many diffs) |
33 | human | 5602 | MAPK10 | mitogen-activated protein k... | NM_001351624.2 | 82% | 81.6% | (many diffs) |
34 | human | 5602 | MAPK10 | mitogen-activated protein k... | NM_138980.4 | 82% | 81.6% | (many diffs) |
35 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_006714268.3 | 82% | 81.6% | (many diffs) |
36 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_011532120.3 | 82% | 81.6% | (many diffs) |
37 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_011532121.3 | 82% | 81.6% | (many diffs) |
38 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008423.2 | 82% | 81.6% | (many diffs) |
39 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008427.2 | 79.9% | 79.9% | (many diffs) |
40 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008428.2 | 79.9% | 79.9% | (many diffs) |
41 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008433.2 | 76.8% | 76.2% | (many diffs) |
42 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008451.2 | 72.9% | 72.9% | 0_1ins342;618G>A |
43 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323302.2 | 72.6% | 84.1% | (many diffs) |
44 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323324.2 | 72.6% | 84.1% | (many diffs) |
45 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323326.2 | 72.6% | 84.1% | (many diffs) |
46 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323327.2 | 72.6% | 84.1% | (many diffs) |
47 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323321.2 | 70.6% | 82.9% | (many diffs) |
48 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323325.2 | 70.6% | 82.9% | (many diffs) |
49 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_139046.4 | 70.6% | 82.9% | (many diffs) |
50 | human | 5599 | MAPK8 | mitogen-activated protein k... | XM_024448080.1 | 70.6% | 82.9% | (many diffs) |
51 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008445.2 | 65.7% | 65.3% | (many diffs) |
52 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008452.2 | 65.5% | 65.6% | 0_1ins435;525G>A |
53 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323328.2 | 65.3% | 75.6% | (many diffs) |
54 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323329.2 | 65.3% | 75.6% | (many diffs) |
55 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323331.2 | 65.3% | 75.6% | (many diffs) |
56 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_139049.4 | 65.3% | 75.6% | (many diffs) |
57 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008447.2 | 59% | 58.6% | (many diffs) |
58 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008448.2 | 59% | 58.6% | (many diffs) |
59 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008453.2 | 58.9% | 57.8% | (many diffs) |
60 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008454.2 | 57.8% | 57.5% | 731_732delGAinsTG;735_735delGins532 |
61 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454149.1 | 57.8% | 57.5% | 731_732delGAinsTG;735_735delGins532 |
62 | human | 5602 | MAPK10 | mitogen-activated protein k... | NM_001318068.1 | 57.1% | 56.8% | (many diffs) |
63 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008449.2 | 57.1% | 56.8% | (many diffs) |
64 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008450.2 | 57.1% | 56.8% | (many diffs) |
65 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454148.1 | 57.1% | 56.8% | (many diffs) |
66 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323320.2 | 51.5% | 60.8% | (many diffs) |
67 | human | 5602 | MAPK10 | mitogen-activated protein k... | XR_001741289.1 | 46.3% | 1_6delGTATTT;736_1425del;1605_1606ins357 | |
68 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | NM_009158.3 | 91.3% | 99.5% | (many diffs) |
69 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | NM_001318131.1 | 89.1% | 97.8% | (many diffs) |
70 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_006534950.4 | 89.1% | 97.8% | (many diffs) |
71 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | NM_001310686.2 | 82.5% | 90.9% | (many diffs) |
72 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_017320890.2 | 82.5% | 90.9% | (many diffs) |
73 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_017320891.2 | 82.5% | 90.9% | (many diffs) |
74 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | NM_001081567.2 | 82.3% | 89.4% | (many diffs) |
75 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_030254516.1 | 82.3% | 89.4% | (many diffs) |
76 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | NM_001310685.1 | 80.4% | 87.9% | (many diffs) |
77 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_017320886.2 | 80.4% | 87.9% | (many diffs) |
78 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_030254519.1 | 80.4% | 89.3% | (many diffs) |
79 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | NM_001318102.1 | 79.9% | 89.4% | (many diffs) |
80 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | NM_001310683.1 | 74.4% | 81.6% | (many diffs) |
81 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_017320888.2 | 74.4% | 81.6% | (many diffs) |
82 | mouse | 26419 | Mapk8 | mitogen-activated protein k... | NM_016700.4 | 72.9% | 84.1% | (many diffs) |
83 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_017320889.2 | 72.5% | 80.1% | (many diffs) |
84 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_011249450.3 | 67% | 72.8% | (many diffs) |
85 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_030254518.1 | 65.2% | 71.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1338
- ORF length:
- 1266
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagcctc catttcttat actactgcag tgaaccaaca ttggatgtga 121 aaattgcctt ttgtcaggga ttcgataaac aagtggatgt gtcatatatt gccaaacatt 181 acaacatgag caaaagcaaa gttgacaacc agttctacag tgtggaagtg ggagactcaa 241 ccttcacagt tctcaagcgc taccagaatc taaagcctat tggctctggg gctcagggca 301 tagtttgtgc cgcgtatgat gctgtccttg acagaaatgt ggccattaag aagctcagca 361 gaccctttca gaaccaaaca catgccaaga gagcgtaccg ggagctggtc ctcatgaagt 421 gtgtgaacca taaaaacatt attagtttat taaatgtctt cacaccccag aaaacgctgg 481 aggagttcca agatgtttac ttagtaatgg aactgatgga tgccaactta tgtcaagtga 541 ttcagatgga attagaccat gagcgaatgt cttacctgct gtaccaaatg ttgtgtggca 601 ttaagcacct ccattctgct ggaattattc acagggattt aaaaccaagt aacattgtag 661 tcaagtctga ttgcacattg aaaatcctgg actttggact ggccaggaca gcaggcacaa 721 gcttcatgat gactccatat gtggtgacac gttattacag agcccctgag gtcatcctgg 781 ggatgggcta caaggagaac gtggatatat ggtctgtggg atgcattatg ggagaaatgg 841 ttcgccacaa aatcctcttt ccaggaaggg actatattga ccagtggaat aaggtaattg 901 aacaactagg aacaccatgt ccagaattca tgaagaaatt gcaacccaca gtaagaaact 961 atgtggagaa tcggcccaag tatgcgggac tcaccttccc caaactcttc ccagattccc 1021 tcttcccagc agacTCCGAG CACAATAAAC TCAAAGCCAG CCAAGCCAGG GACTTGTTGT 1081 CAAAGATGCT AGTGATTGAC CCAGCAAAAA GAATATCAGT GGACGACGCC TTACAGCATC 1141 CCTACATCAA CGTCTGGTAT GACCCAGCCG AAGTGGAGGC GCCTCCACCT CAGATATATG 1201 ACAAGCAGTT GGATGAAAGA GAACACACAA TTGAAGAATG GAAAGAACTT ATCTACAAGG 1261 AAGTAATGAA TTCAGAAGAA AAGACTAAAA ATGGTGTAGT AAAAGGACAG CCTTCTCCTT 1321 CAGCACAGGT GCAGCAGTAG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1381 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1441 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCGT CCACGCTTTT 1501 ACTCGGCAGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt