Construct: ORF TRCN0000491457

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020400.2_s317c1
DNA Barcode:
CCGTCCACGCTTTTACTCGGCAGT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
MAPK10 (5602)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491457
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5602 MAPK10 mitogen-activated protein k... NM_002753.5 99.9% 100% 960G>A
2 human 5602 MAPK10 mitogen-activated protein k... XM_005263135.4 99.9% 100% 960G>A
3 human 5602 MAPK10 mitogen-activated protein k... XM_017008429.2 99.9% 100% 960G>A
4 human 5602 MAPK10 mitogen-activated protein k... XM_017008430.2 99.9% 100% 960G>A
5 human 5602 MAPK10 mitogen-activated protein k... XM_017008432.2 99.9% 100% 960G>A
6 human 5602 MAPK10 mitogen-activated protein k... XM_024454144.1 99.9% 100% 960G>A
7 human 5602 MAPK10 mitogen-activated protein k... XM_024454145.1 99.9% 100% 960G>A
8 human 5602 MAPK10 mitogen-activated protein k... XM_024454146.1 99.9% 100% 960G>A
9 human 5602 MAPK10 mitogen-activated protein k... XM_024454147.1 99.9% 100% 960G>A
10 human 5602 MAPK10 mitogen-activated protein k... XM_006714269.3 97.5% 98.1% (many diffs)
11 human 5602 MAPK10 mitogen-activated protein k... NM_001351625.2 90.9% 90.9% 0_1ins114;846G>A
12 human 5602 MAPK10 mitogen-activated protein k... XM_017008434.2 90.9% 90.9% 0_1ins114;846G>A
13 human 5602 MAPK10 mitogen-activated protein k... XM_017008435.2 90.9% 90.9% 0_1ins114;846G>A
14 human 5602 MAPK10 mitogen-activated protein k... XM_017008436.2 90.9% 90.9% 0_1ins114;846G>A
15 human 5602 MAPK10 mitogen-activated protein k... XM_017008437.2 90.9% 90.9% 0_1ins114;846G>A
16 human 5602 MAPK10 mitogen-activated protein k... NM_138982.4 90.1% 89.8% 960G>A;1249_1250insCAGCA;1262_1392del
17 human 5602 MAPK10 mitogen-activated protein k... XM_005263129.3 90.1% 89.8% 960G>A;1249_1250insCAGCA;1262_1392del
18 human 5602 MAPK10 mitogen-activated protein k... XM_005263130.3 90.1% 89.8% 960G>A;1249_1250insCAGCA;1262_1392del
19 human 5602 MAPK10 mitogen-activated protein k... XM_011532117.3 90.1% 89.8% 960G>A;1249_1250insCAGCA;1262_1392del
20 human 5602 MAPK10 mitogen-activated protein k... XM_011532118.3 90.1% 89.8% 960G>A;1249_1250insCAGCA;1262_1392del
21 human 5602 MAPK10 mitogen-activated protein k... XM_017008420.2 90.1% 89.8% 960G>A;1249_1250insCAGCA;1262_1392del
22 human 5602 MAPK10 mitogen-activated protein k... XM_024454139.1 90.1% 89.8% 960G>A;1249_1250insCAGCA;1262_1392del
23 human 5602 MAPK10 mitogen-activated protein k... XM_024454140.1 90.1% 89.8% 960G>A;1249_1250insCAGCA;1262_1392del
24 human 5602 MAPK10 mitogen-activated protein k... XM_024454141.1 90.1% 89.8% 960G>A;1249_1250insCAGCA;1262_1392del
25 human 5602 MAPK10 mitogen-activated protein k... XM_024454142.1 90.1% 89.8% 960G>A;1249_1250insCAGCA;1262_1392del
26 human 5602 MAPK10 mitogen-activated protein k... XM_024454143.1 90.1% 89.8% 960G>A;1249_1250insCAGCA;1262_1392del
27 human 5602 MAPK10 mitogen-activated protein k... NM_001363657.2 88.6% 89% (many diffs)
28 human 5602 MAPK10 mitogen-activated protein k... NM_001318067.1 88.1% 88.1% (many diffs)
29 human 5602 MAPK10 mitogen-activated protein k... XM_005263131.3 88.1% 88.1% (many diffs)
30 human 5602 MAPK10 mitogen-activated protein k... NM_001318069.2 87.5% 89.8% 960G>A;1249_1250insCAGCA;1262_1434del
31 human 5602 MAPK10 mitogen-activated protein k... XM_017008441.2 85.2% 85% 0_1ins114;616_617ins72;774G>A
32 human 5602 MAPK10 mitogen-activated protein k... XM_017008422.2 85% 84.4% (many diffs)
33 human 5602 MAPK10 mitogen-activated protein k... NM_001351624.2 82% 81.6% (many diffs)
34 human 5602 MAPK10 mitogen-activated protein k... NM_138980.4 82% 81.6% (many diffs)
35 human 5602 MAPK10 mitogen-activated protein k... XM_006714268.3 82% 81.6% (many diffs)
36 human 5602 MAPK10 mitogen-activated protein k... XM_011532120.3 82% 81.6% (many diffs)
37 human 5602 MAPK10 mitogen-activated protein k... XM_011532121.3 82% 81.6% (many diffs)
38 human 5602 MAPK10 mitogen-activated protein k... XM_017008423.2 82% 81.6% (many diffs)
39 human 5602 MAPK10 mitogen-activated protein k... XM_017008427.2 79.9% 79.9% (many diffs)
40 human 5602 MAPK10 mitogen-activated protein k... XM_017008428.2 79.9% 79.9% (many diffs)
41 human 5602 MAPK10 mitogen-activated protein k... XM_017008433.2 76.8% 76.2% (many diffs)
42 human 5602 MAPK10 mitogen-activated protein k... XM_017008451.2 72.9% 72.9% 0_1ins342;618G>A
43 human 5599 MAPK8 mitogen-activated protein k... NM_001323302.2 72.6% 84.1% (many diffs)
44 human 5599 MAPK8 mitogen-activated protein k... NM_001323324.2 72.6% 84.1% (many diffs)
45 human 5599 MAPK8 mitogen-activated protein k... NM_001323326.2 72.6% 84.1% (many diffs)
46 human 5599 MAPK8 mitogen-activated protein k... NM_001323327.2 72.6% 84.1% (many diffs)
47 human 5599 MAPK8 mitogen-activated protein k... NM_001323321.2 70.6% 82.9% (many diffs)
48 human 5599 MAPK8 mitogen-activated protein k... NM_001323325.2 70.6% 82.9% (many diffs)
49 human 5599 MAPK8 mitogen-activated protein k... NM_139046.4 70.6% 82.9% (many diffs)
50 human 5599 MAPK8 mitogen-activated protein k... XM_024448080.1 70.6% 82.9% (many diffs)
51 human 5602 MAPK10 mitogen-activated protein k... XM_017008445.2 65.7% 65.3% (many diffs)
52 human 5602 MAPK10 mitogen-activated protein k... XM_017008452.2 65.5% 65.6% 0_1ins435;525G>A
53 human 5599 MAPK8 mitogen-activated protein k... NM_001323328.2 65.3% 75.6% (many diffs)
54 human 5599 MAPK8 mitogen-activated protein k... NM_001323329.2 65.3% 75.6% (many diffs)
55 human 5599 MAPK8 mitogen-activated protein k... NM_001323331.2 65.3% 75.6% (many diffs)
56 human 5599 MAPK8 mitogen-activated protein k... NM_139049.4 65.3% 75.6% (many diffs)
57 human 5602 MAPK10 mitogen-activated protein k... XM_017008447.2 59% 58.6% (many diffs)
58 human 5602 MAPK10 mitogen-activated protein k... XM_017008448.2 59% 58.6% (many diffs)
59 human 5602 MAPK10 mitogen-activated protein k... XM_017008453.2 58.9% 57.8% (many diffs)
60 human 5602 MAPK10 mitogen-activated protein k... XM_017008454.2 57.8% 57.5% 731_732delGAinsTG;735_735delGins532
61 human 5602 MAPK10 mitogen-activated protein k... XM_024454149.1 57.8% 57.5% 731_732delGAinsTG;735_735delGins532
62 human 5602 MAPK10 mitogen-activated protein k... NM_001318068.1 57.1% 56.8% (many diffs)
63 human 5602 MAPK10 mitogen-activated protein k... XM_017008449.2 57.1% 56.8% (many diffs)
64 human 5602 MAPK10 mitogen-activated protein k... XM_017008450.2 57.1% 56.8% (many diffs)
65 human 5602 MAPK10 mitogen-activated protein k... XM_024454148.1 57.1% 56.8% (many diffs)
66 human 5599 MAPK8 mitogen-activated protein k... NM_001323320.2 51.5% 60.8% (many diffs)
67 human 5602 MAPK10 mitogen-activated protein k... XR_001741289.1 46.3% 1_6delGTATTT;736_1425del;1605_1606ins357
68 mouse 26414 Mapk10 mitogen-activated protein k... NM_009158.3 91.3% 99.5% (many diffs)
69 mouse 26414 Mapk10 mitogen-activated protein k... NM_001318131.1 89.1% 97.8% (many diffs)
70 mouse 26414 Mapk10 mitogen-activated protein k... XM_006534950.4 89.1% 97.8% (many diffs)
71 mouse 26414 Mapk10 mitogen-activated protein k... NM_001310686.2 82.5% 90.9% (many diffs)
72 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320890.2 82.5% 90.9% (many diffs)
73 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320891.2 82.5% 90.9% (many diffs)
74 mouse 26414 Mapk10 mitogen-activated protein k... NM_001081567.2 82.3% 89.4% (many diffs)
75 mouse 26414 Mapk10 mitogen-activated protein k... XM_030254516.1 82.3% 89.4% (many diffs)
76 mouse 26414 Mapk10 mitogen-activated protein k... NM_001310685.1 80.4% 87.9% (many diffs)
77 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320886.2 80.4% 87.9% (many diffs)
78 mouse 26414 Mapk10 mitogen-activated protein k... XM_030254519.1 80.4% 89.3% (many diffs)
79 mouse 26414 Mapk10 mitogen-activated protein k... NM_001318102.1 79.9% 89.4% (many diffs)
80 mouse 26414 Mapk10 mitogen-activated protein k... NM_001310683.1 74.4% 81.6% (many diffs)
81 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320888.2 74.4% 81.6% (many diffs)
82 mouse 26419 Mapk8 mitogen-activated protein k... NM_016700.4 72.9% 84.1% (many diffs)
83 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320889.2 72.5% 80.1% (many diffs)
84 mouse 26414 Mapk10 mitogen-activated protein k... XM_011249450.3 67% 72.8% (many diffs)
85 mouse 26414 Mapk10 mitogen-activated protein k... XM_030254518.1 65.2% 71.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1338
ORF length:
1266
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgagcctc catttcttat actactgcag tgaaccaaca ttggatgtga
121aaattgcctt ttgtcaggga ttcgataaac aagtggatgt gtcatatatt gccaaacatt
181acaacatgag caaaagcaaa gttgacaacc agttctacag tgtggaagtg ggagactcaa
241ccttcacagt tctcaagcgc taccagaatc taaagcctat tggctctggg gctcagggca
301tagtttgtgc cgcgtatgat gctgtccttg acagaaatgt ggccattaag aagctcagca
361gaccctttca gaaccaaaca catgccaaga gagcgtaccg ggagctggtc ctcatgaagt
421gtgtgaacca taaaaacatt attagtttat taaatgtctt cacaccccag aaaacgctgg
481aggagttcca agatgtttac ttagtaatgg aactgatgga tgccaactta tgtcaagtga
541ttcagatgga attagaccat gagcgaatgt cttacctgct gtaccaaatg ttgtgtggca
601ttaagcacct ccattctgct ggaattattc acagggattt aaaaccaagt aacattgtag
661tcaagtctga ttgcacattg aaaatcctgg actttggact ggccaggaca gcaggcacaa
721gcttcatgat gactccatat gtggtgacac gttattacag agcccctgag gtcatcctgg
781ggatgggcta caaggagaac gtggatatat ggtctgtggg atgcattatg ggagaaatgg
841ttcgccacaa aatcctcttt ccaggaaggg actatattga ccagtggaat aaggtaattg
901aacaactagg aacaccatgt ccagaattca tgaagaaatt gcaacccaca gtaagaaact
961atgtggagaa tcggcccaag tatgcgggac tcaccttccc caaactcttc ccagattccc
1021tcttcccagc agacTCCGAG CACAATAAAC TCAAAGCCAG CCAAGCCAGG GACTTGTTGT
1081CAAAGATGCT AGTGATTGAC CCAGCAAAAA GAATATCAGT GGACGACGCC TTACAGCATC
1141CCTACATCAA CGTCTGGTAT GACCCAGCCG AAGTGGAGGC GCCTCCACCT CAGATATATG
1201ACAAGCAGTT GGATGAAAGA GAACACACAA TTGAAGAATG GAAAGAACTT ATCTACAAGG
1261AAGTAATGAA TTCAGAAGAA AAGACTAAAA ATGGTGTAGT AAAAGGACAG CCTTCTCCTT
1321CAGCACAGGT GCAGCAGTAG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1381CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1441AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCGT CCACGCTTTT
1501ACTCGGCAGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)