Construct: ORF TRCN0000475774
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017578.1_s317c1
- Derived from:
- ccsbBroadEn_11058
- DNA Barcode:
- GTGAGTTGCCAATCATGCAATTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAPK10 (5602)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475774
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5602 | MAPK10 | mitogen-activated protein k... | NM_001318068.1 | 100% | 100% | |
| 2 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008449.2 | 100% | 100% | |
| 3 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008450.2 | 100% | 100% | |
| 4 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454148.1 | 100% | 100% | |
| 5 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008447.2 | 96.8% | 97.4% | (many diffs) |
| 6 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008448.2 | 96.8% | 97.4% | (many diffs) |
| 7 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008445.2 | 88.3% | 88.8% | (many diffs) |
| 8 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008452.2 | 82.8% | 82.7% | (many diffs) |
| 9 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008451.2 | 75.6% | 75.4% | (many diffs) |
| 10 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008427.2 | 74.8% | 74.8% | 1_321del |
| 11 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008428.2 | 74.8% | 74.8% | 1_321del |
| 12 | human | 5602 | MAPK10 | mitogen-activated protein k... | NM_001351624.2 | 72.6% | 73% | (many diffs) |
| 13 | human | 5602 | MAPK10 | mitogen-activated protein k... | NM_138980.4 | 72.6% | 73% | (many diffs) |
| 14 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_006714268.3 | 72.6% | 73% | (many diffs) |
| 15 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_011532120.3 | 72.6% | 73% | (many diffs) |
| 16 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_011532121.3 | 72.6% | 73% | (many diffs) |
| 17 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008423.2 | 72.6% | 73% | (many diffs) |
| 18 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008433.2 | 69.2% | 69% | 1_321del;616_617ins72 |
| 19 | human | 5602 | MAPK10 | mitogen-activated protein k... | NM_001318067.1 | 68.7% | 68.7% | 1_435del |
| 20 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_005263131.3 | 68.7% | 68.7% | 1_435del |
| 21 | human | 5602 | MAPK10 | mitogen-activated protein k... | NM_138982.4 | 66.7% | 67% | (many diffs) |
| 22 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_005263129.3 | 66.7% | 67% | (many diffs) |
| 23 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_005263130.3 | 66.7% | 67% | (many diffs) |
| 24 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_011532117.3 | 66.7% | 67% | (many diffs) |
| 25 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_011532118.3 | 66.7% | 67% | (many diffs) |
| 26 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008420.2 | 66.7% | 67% | (many diffs) |
| 27 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454139.1 | 66.7% | 67% | (many diffs) |
| 28 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454140.1 | 66.7% | 67% | (many diffs) |
| 29 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454141.1 | 66.7% | 67% | (many diffs) |
| 30 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454142.1 | 66.7% | 67% | (many diffs) |
| 31 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454143.1 | 66.7% | 67% | (many diffs) |
| 32 | human | 5602 | MAPK10 | mitogen-activated protein k... | NM_001318069.2 | 64.8% | 67% | (many diffs) |
| 33 | human | 5602 | MAPK10 | mitogen-activated protein k... | NM_001363657.2 | 64.3% | 63.8% | 1_321del;1136_1140delCAGCA;1152_1153ins131 |
| 34 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008422.2 | 63.5% | 63.3% | 1_435del;730_731ins72 |
| 35 | human | 5602 | MAPK10 | mitogen-activated protein k... | NM_001351625.2 | 62.2% | 61.9% | (many diffs) |
| 36 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008434.2 | 62.2% | 61.9% | (many diffs) |
| 37 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008435.2 | 62.2% | 61.9% | (many diffs) |
| 38 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008436.2 | 62.2% | 61.9% | (many diffs) |
| 39 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008437.2 | 62.2% | 61.9% | (many diffs) |
| 40 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_006714269.3 | 59.1% | 58.6% | 1_435del;1250_1254delCAGCA;1266_1267ins131 |
| 41 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008441.2 | 58.7% | 57.9% | (many diffs) |
| 42 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323322.2 | 58.2% | 67.9% | (many diffs) |
| 43 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323323.2 | 58.2% | 67.9% | (many diffs) |
| 44 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323330.2 | 58.2% | 67.9% | (many diffs) |
| 45 | human | 5599 | MAPK8 | mitogen-activated protein k... | XM_024448079.1 | 58.2% | 67.9% | (many diffs) |
| 46 | human | 5602 | MAPK10 | mitogen-activated protein k... | NM_002753.5 | 57.1% | 56.8% | (many diffs) |
| 47 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_005263135.4 | 57.1% | 56.8% | (many diffs) |
| 48 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008429.2 | 57.1% | 56.8% | (many diffs) |
| 49 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008430.2 | 57.1% | 56.8% | (many diffs) |
| 50 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008432.2 | 57.1% | 56.8% | (many diffs) |
| 51 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454144.1 | 57.1% | 56.8% | (many diffs) |
| 52 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454145.1 | 57.1% | 56.8% | (many diffs) |
| 53 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454146.1 | 57.1% | 56.8% | (many diffs) |
| 54 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454147.1 | 57.1% | 56.8% | (many diffs) |
| 55 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323321.2 | 51.4% | 59.1% | (many diffs) |
| 56 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_001323325.2 | 51.4% | 59.1% | (many diffs) |
| 57 | human | 5599 | MAPK8 | mitogen-activated protein k... | NM_139046.4 | 51.4% | 59.1% | (many diffs) |
| 58 | human | 5599 | MAPK8 | mitogen-activated protein k... | XM_024448080.1 | 51.4% | 59.1% | (many diffs) |
| 59 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008453.2 | 22.4% | 21.3% | (many diffs) |
| 60 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008454.2 | 21.3% | 21.1% | 1_435del;731_732delGAinsTT;735_735delGins658 |
| 61 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454149.1 | 21.3% | 21.1% | 1_435del;731_732delGAinsTT;735_735delGins658 |
| 62 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_030254518.1 | 74.1% | 81% | (many diffs) |
| 63 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_011249450.3 | 72.3% | 79.7% | (many diffs) |
| 64 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_017320889.2 | 67.8% | 74.1% | (many diffs) |
| 65 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | NM_001310683.1 | 66.2% | 73% | (many diffs) |
| 66 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_017320888.2 | 66.2% | 73% | (many diffs) |
| 67 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | NM_001310685.1 | 62.2% | 68.1% | (many diffs) |
| 68 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_017320886.2 | 62.2% | 68.1% | (many diffs) |
| 69 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | NM_001081567.2 | 60.8% | 67% | (many diffs) |
| 70 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_030254516.1 | 60.8% | 67% | (many diffs) |
| 71 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | NM_001318102.1 | 59% | 67% | (many diffs) |
| 72 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_030254519.1 | 57.8% | 63.1% | (many diffs) |
| 73 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | NM_001310686.2 | 56.2% | 61.9% | (many diffs) |
| 74 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_017320890.2 | 56.2% | 61.9% | (many diffs) |
| 75 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_017320891.2 | 56.2% | 61.9% | (many diffs) |
| 76 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | NM_001318131.1 | 53.1% | 57.9% | (many diffs) |
| 77 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | XM_006534950.4 | 53.1% | 57.9% | (many diffs) |
| 78 | mouse | 26414 | Mapk10 | mitogen-activated protein k... | NM_009158.3 | 51.7% | 56.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1026
- ORF length:
- 957
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaactgatg gatgccaact tatgtcaagt gattcagatg gaattagacc 121 atgagcgaat gtcttacctg ctgtaccaaa tgttgtgtgg cattaagcac ctccattctg 181 ctggaattat tcacagggat ttaaaaccaa gtaacattgt agtcaagtct gattgcacat 241 tgaaaatcct ggactttgga ctggccagga cagcaggcac aagcttcatg atgactccat 301 atgtggtgac acgttattac agagcccctg aggtcatcct ggggatgggc tacaaggaga 361 acgttgacat gtggtcagta gggtgcatca tgggagaaat gataaaaggt gcagtgctgt 421 ttcctggcac tgatcatatt gaccagtgga ataaggtaat tgaacaacta ggaacaccat 481 gtccagaatt catgaagaaa ttgcaaccca cagtaagaaa ctatgtggag aatcggccca 541 agtatgcggg actcaccttc cccaaactct tcccagattc cctcttccca gcggactccg 601 agcacaataa actcaaagcc agccaagcca gggacttgtt gtcaaagatg ctagtgattg 661 acccagcaaa aagaatatca gtggacgacg ccttacagca tccctacatc aacgtctggt 721 atgacccagc cgaagtggag gcgcctccac cTCAGATATA TGACAAGCAG TTGGATGAAA 781 GAGAACACAC AATTGAAGAA TGGAAAGAAC TTATCTACAA GGAAGTAATG AATTCAGAAG 841 AAAAGACTAA AAATGGTGTA GTAAAAGGAC AGCCTTCTCC TTCAGGTGCA GCAGTGAACA 901 GCAGTGAGAG TCTCCCTCCA TCCTCGTCTG TCAATGACAT CTCCTCCATG TCCACCGACC 961 AGACCCTGGC ATCTGACACT GACAGCAGCC TGGAAGCCTC GGCAGGACCC CTGGGTTGTT 1021 GCAGGTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1081 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1141 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGTGAGTTGC CAATCATGCA ATTTAACGCG 1201 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt