Construct: ORF TRCN0000466133

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005216.2_s317c1
Derived from:
ccsbBroadEn_14805
DNA Barcode:
TCATAAGCAAACAGGTTCCCACAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MAPK10 (5602)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466133
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5602 MAPK10 mitogen-activated protein k... XM_017008447.2 100% 100%
2 human 5602 MAPK10 mitogen-activated protein k... XM_017008448.2 100% 100%
3 human 5602 MAPK10 mitogen-activated protein k... NM_001318068.1 96.8% 97.4% (many diffs)
4 human 5602 MAPK10 mitogen-activated protein k... XM_017008449.2 96.8% 97.4% (many diffs)
5 human 5602 MAPK10 mitogen-activated protein k... XM_017008450.2 96.8% 97.4% (many diffs)
6 human 5602 MAPK10 mitogen-activated protein k... XM_024454148.1 96.8% 97.4% (many diffs)
7 human 5602 MAPK10 mitogen-activated protein k... XM_017008445.2 91.1% 91.1% 1_93del
8 human 5602 MAPK10 mitogen-activated protein k... XM_017008452.2 85.8% 85.2% 815_819delCAGCA;831_832ins131
9 human 5602 MAPK10 mitogen-activated protein k... XM_017008451.2 78.2% 77.7% 1_93del;908_912delCAGCA;924_925ins131
10 human 5602 MAPK10 mitogen-activated protein k... NM_001351624.2 74.8% 74.8% 1_321del
11 human 5602 MAPK10 mitogen-activated protein k... NM_138980.4 74.8% 74.8% 1_321del
12 human 5602 MAPK10 mitogen-activated protein k... XM_006714268.3 74.8% 74.8% 1_321del
13 human 5602 MAPK10 mitogen-activated protein k... XM_011532120.3 74.8% 74.8% 1_321del
14 human 5602 MAPK10 mitogen-activated protein k... XM_011532121.3 74.8% 74.8% 1_321del
15 human 5602 MAPK10 mitogen-activated protein k... XM_017008423.2 74.8% 74.8% 1_321del
16 human 5602 MAPK10 mitogen-activated protein k... XM_017008427.2 72.6% 73% (many diffs)
17 human 5602 MAPK10 mitogen-activated protein k... XM_017008428.2 72.6% 73% (many diffs)
18 human 5602 MAPK10 mitogen-activated protein k... XM_017008433.2 69.2% 69% 1_321del;616_617ins72
19 human 5602 MAPK10 mitogen-activated protein k... NM_138982.4 68.7% 68.7% 1_435del
20 human 5602 MAPK10 mitogen-activated protein k... XM_005263129.3 68.7% 68.7% 1_435del
21 human 5602 MAPK10 mitogen-activated protein k... XM_005263130.3 68.7% 68.7% 1_435del
22 human 5602 MAPK10 mitogen-activated protein k... XM_011532117.3 68.7% 68.7% 1_435del
23 human 5602 MAPK10 mitogen-activated protein k... XM_011532118.3 68.7% 68.7% 1_435del
24 human 5602 MAPK10 mitogen-activated protein k... XM_017008420.2 68.7% 68.7% 1_435del
25 human 5602 MAPK10 mitogen-activated protein k... XM_024454139.1 68.7% 68.7% 1_435del
26 human 5602 MAPK10 mitogen-activated protein k... XM_024454140.1 68.7% 68.7% 1_435del
27 human 5602 MAPK10 mitogen-activated protein k... XM_024454141.1 68.7% 68.7% 1_435del
28 human 5602 MAPK10 mitogen-activated protein k... XM_024454142.1 68.7% 68.7% 1_435del
29 human 5602 MAPK10 mitogen-activated protein k... XM_024454143.1 68.7% 68.7% 1_435del
30 human 5602 MAPK10 mitogen-activated protein k... NM_001318067.1 66.7% 67% (many diffs)
31 human 5602 MAPK10 mitogen-activated protein k... XM_005263131.3 66.7% 67% (many diffs)
32 human 5602 MAPK10 mitogen-activated protein k... NM_001318069.2 66.7% 68.7% 1_435del;1393_1434del
33 human 5602 MAPK10 mitogen-activated protein k... NM_001351625.2 64.3% 63.8% 1_321del;1136_1140delCAGCA;1152_1153ins131
34 human 5602 MAPK10 mitogen-activated protein k... XM_017008434.2 64.3% 63.8% 1_321del;1136_1140delCAGCA;1152_1153ins131
35 human 5602 MAPK10 mitogen-activated protein k... XM_017008435.2 64.3% 63.8% 1_321del;1136_1140delCAGCA;1152_1153ins131
36 human 5602 MAPK10 mitogen-activated protein k... XM_017008436.2 64.3% 63.8% 1_321del;1136_1140delCAGCA;1152_1153ins131
37 human 5602 MAPK10 mitogen-activated protein k... XM_017008437.2 64.3% 63.8% 1_321del;1136_1140delCAGCA;1152_1153ins131
38 human 5602 MAPK10 mitogen-activated protein k... XM_017008422.2 63.5% 63.3% 1_435del;730_731ins72
39 human 5602 MAPK10 mitogen-activated protein k... NM_001363657.2 62.2% 61.9% (many diffs)
40 human 5602 MAPK10 mitogen-activated protein k... NM_002753.5 59.1% 58.6% 1_435del;1250_1254delCAGCA;1266_1267ins131
41 human 5602 MAPK10 mitogen-activated protein k... XM_005263135.4 59.1% 58.6% 1_435del;1250_1254delCAGCA;1266_1267ins131
42 human 5602 MAPK10 mitogen-activated protein k... XM_017008429.2 59.1% 58.6% 1_435del;1250_1254delCAGCA;1266_1267ins131
43 human 5602 MAPK10 mitogen-activated protein k... XM_017008430.2 59.1% 58.6% 1_435del;1250_1254delCAGCA;1266_1267ins131
44 human 5602 MAPK10 mitogen-activated protein k... XM_017008432.2 59.1% 58.6% 1_435del;1250_1254delCAGCA;1266_1267ins131
45 human 5602 MAPK10 mitogen-activated protein k... XM_024454144.1 59.1% 58.6% 1_435del;1250_1254delCAGCA;1266_1267ins131
46 human 5602 MAPK10 mitogen-activated protein k... XM_024454145.1 59.1% 58.6% 1_435del;1250_1254delCAGCA;1266_1267ins131
47 human 5602 MAPK10 mitogen-activated protein k... XM_024454146.1 59.1% 58.6% 1_435del;1250_1254delCAGCA;1266_1267ins131
48 human 5602 MAPK10 mitogen-activated protein k... XM_024454147.1 59.1% 58.6% 1_435del;1250_1254delCAGCA;1266_1267ins131
49 human 5599 MAPK8 mitogen-activated protein k... NM_001323328.2 58.8% 67.9% (many diffs)
50 human 5599 MAPK8 mitogen-activated protein k... NM_001323329.2 58.8% 67.9% (many diffs)
51 human 5599 MAPK8 mitogen-activated protein k... NM_001323331.2 58.8% 67.9% (many diffs)
52 human 5599 MAPK8 mitogen-activated protein k... NM_139049.4 58.8% 67.9% (many diffs)
53 human 5602 MAPK10 mitogen-activated protein k... XM_017008441.2 58.7% 57.9% (many diffs)
54 human 5602 MAPK10 mitogen-activated protein k... XM_006714269.3 57.1% 56.8% (many diffs)
55 human 5599 MAPK8 mitogen-activated protein k... NM_001323302.2 52% 59.1% (many diffs)
56 human 5599 MAPK8 mitogen-activated protein k... NM_001323324.2 52% 59.1% (many diffs)
57 human 5599 MAPK8 mitogen-activated protein k... NM_001323326.2 52% 59.1% (many diffs)
58 human 5599 MAPK8 mitogen-activated protein k... NM_001323327.2 52% 59.1% (many diffs)
59 human 5602 MAPK10 mitogen-activated protein k... XM_017008453.2 22.3% 21.3% (many diffs)
60 human 5602 MAPK10 mitogen-activated protein k... XM_017008454.2 21.3% 21.1% 1_435del;731_732delGAinsTG;735_735delGins658
61 human 5602 MAPK10 mitogen-activated protein k... XM_024454149.1 21.3% 21.1% 1_435del;731_732delGAinsTG;735_735delGins658
62 mouse 26414 Mapk10 mitogen-activated protein k... XM_011249450.3 74.2% 81.7% (many diffs)
63 mouse 26414 Mapk10 mitogen-activated protein k... XM_030254518.1 72% 80% (many diffs)
64 mouse 26414 Mapk10 mitogen-activated protein k... NM_001310683.1 67.9% 74.8% (many diffs)
65 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320888.2 67.9% 74.8% (many diffs)
66 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320889.2 65.9% 73.2% (many diffs)
67 mouse 26414 Mapk10 mitogen-activated protein k... NM_001081567.2 62.4% 68.7% (many diffs)
68 mouse 26414 Mapk10 mitogen-activated protein k... XM_030254516.1 62.4% 68.7% (many diffs)
69 mouse 26414 Mapk10 mitogen-activated protein k... NM_001310685.1 60.6% 67.2% (many diffs)
70 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320886.2 60.6% 67.2% (many diffs)
71 mouse 26414 Mapk10 mitogen-activated protein k... NM_001318102.1 60.5% 68.7% (many diffs)
72 mouse 26419 Mapk8 mitogen-activated protein k... NM_001310454.1 59.3% 67.6% (many diffs)
73 mouse 26414 Mapk10 mitogen-activated protein k... NM_001310686.2 57.9% 63.8% (many diffs)
74 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320890.2 57.9% 63.8% (many diffs)
75 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320891.2 57.9% 63.8% (many diffs)
76 mouse 26414 Mapk10 mitogen-activated protein k... XM_030254519.1 56% 62.2% (many diffs)
77 mouse 26414 Mapk10 mitogen-activated protein k... NM_009158.3 53.2% 58.6% (many diffs)
78 mouse 26419 Mapk8 mitogen-activated protein k... NM_016700.4 52% 59.1% (many diffs)
79 mouse 26414 Mapk10 mitogen-activated protein k... NM_001318131.1 51.4% 57.1% (many diffs)
80 mouse 26414 Mapk10 mitogen-activated protein k... XM_006534950.4 51.4% 57.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1023
ORF length:
957
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga actgatggat gccaacttat gtcaagtgat tcagatggaa ttagaccatg
121agcgaatgtc ttacctgctg taccaaatgt tgtgtggcat taagcacctc cattctgctg
181gaattattca cagggattta aaaccaagta acattgtagt caagtctgat tgcacattga
241aaatcctgga ctttggactg gccaggacag caggcacaag cttcatgatg actccatatg
301tggtgacacg ttattacaga gcccctgagg tcatcctggg gatgggctac aaggagaacg
361tggatatatg gtctgtggga tgcattatgg gagaaatggt tcgccacaaa atcctctttc
421caggaaggga ctatattgac cagtggaata aggtaattga acaactagga acaccatgtc
481cagaattcat gaagaaattg caacccacag taagaaacta tgtggagaat cggcccaagt
541atgcgggact caccttcccc aaactcttcc cagattccct cttcccagcg gactccgagc
601acaataaact caaagccagc caagccaggg acttgttgtc aaagatgcta gtgattgacc
661cagcaaaaag aatatcagtg gacgacgcct tacagcatcc ctacatcaac gtctggtatg
721acccagccga agtggaggcg cctccaccTC AGATATATGA CAAGCAGTTG GATGAAAGAG
781AACACACAAT TGAAGAATGG AAAGAACTTA TCTACAAGGA AGTAATGAAT TCAGAAGAAA
841AGACTAAAAA TGGTGTAGTA AAAGGACAGC CTTCTCCTTC AGGTGCAGCA GTGAACAGCA
901GTGAGAGTCT CCCTCCATCC TCGTCTGTCA ATGACATCTC CTCCATGTCC ACCGACCAGA
961CCCTGGCATC TGACACTGAC AGCAGCCTGG AAGCCTCGGC AGGACCCCTG GGTTGTTGCA
1021GGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1081TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
1141GGCTTTATAT ATCTTGTGGA AAGGACGATC ATAAGCAAAC AGGTTCCCAC AGACGCGTTA
1201AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)