Construct: ORF TRCN0000487812

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020470.1_s317c1
DNA Barcode:
TGCAGATTATTTTACTCCTCGACG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
MAPK10 (5602)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487812
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5602 MAPK10 mitogen-activated protein k... NM_138982.4 99.6% 89.8% 1249_1250insCAGCA
2 human 5602 MAPK10 mitogen-activated protein k... XM_005263129.3 99.6% 89.8% 1249_1250insCAGCA
3 human 5602 MAPK10 mitogen-activated protein k... XM_005263130.3 99.6% 89.8% 1249_1250insCAGCA
4 human 5602 MAPK10 mitogen-activated protein k... XM_011532117.3 99.6% 89.8% 1249_1250insCAGCA
5 human 5602 MAPK10 mitogen-activated protein k... XM_011532118.3 99.6% 89.8% 1249_1250insCAGCA
6 human 5602 MAPK10 mitogen-activated protein k... XM_017008420.2 99.6% 89.8% 1249_1250insCAGCA
7 human 5602 MAPK10 mitogen-activated protein k... XM_024454139.1 99.6% 89.8% 1249_1250insCAGCA
8 human 5602 MAPK10 mitogen-activated protein k... XM_024454140.1 99.6% 89.8% 1249_1250insCAGCA
9 human 5602 MAPK10 mitogen-activated protein k... XM_024454141.1 99.6% 89.8% 1249_1250insCAGCA
10 human 5602 MAPK10 mitogen-activated protein k... XM_024454142.1 99.6% 89.8% 1249_1250insCAGCA
11 human 5602 MAPK10 mitogen-activated protein k... XM_024454143.1 99.6% 89.8% 1249_1250insCAGCA
12 human 5602 MAPK10 mitogen-activated protein k... NM_001318067.1 97.5% 88.1% (many diffs)
13 human 5602 MAPK10 mitogen-activated protein k... XM_005263131.3 97.5% 88.1% (many diffs)
14 human 5602 MAPK10 mitogen-activated protein k... NM_001318069.2 96.7% 89.8% 1249_1250insCAGCA;1393_1434del
15 human 5602 MAPK10 mitogen-activated protein k... XM_017008422.2 94.4% 84.4% 730_731ins72;1177_1178insCAGCA
16 human 5602 MAPK10 mitogen-activated protein k... NM_001351624.2 91.4% 81.6% 0_1ins114;1135_1136insCAGCA
17 human 5602 MAPK10 mitogen-activated protein k... NM_138980.4 91.4% 81.6% 0_1ins114;1135_1136insCAGCA
18 human 5602 MAPK10 mitogen-activated protein k... XM_006714268.3 91.4% 81.6% 0_1ins114;1135_1136insCAGCA
19 human 5602 MAPK10 mitogen-activated protein k... XM_011532120.3 91.4% 81.6% 0_1ins114;1135_1136insCAGCA
20 human 5602 MAPK10 mitogen-activated protein k... XM_011532121.3 91.4% 81.6% 0_1ins114;1135_1136insCAGCA
21 human 5602 MAPK10 mitogen-activated protein k... XM_017008423.2 91.4% 81.6% 0_1ins114;1135_1136insCAGCA
22 human 5602 MAPK10 mitogen-activated protein k... NM_002753.5 90.6% 100% 1266_1267ins131
23 human 5602 MAPK10 mitogen-activated protein k... XM_005263135.4 90.6% 100% 1266_1267ins131
24 human 5602 MAPK10 mitogen-activated protein k... XM_017008429.2 90.6% 100% 1266_1267ins131
25 human 5602 MAPK10 mitogen-activated protein k... XM_017008430.2 90.6% 100% 1266_1267ins131
26 human 5602 MAPK10 mitogen-activated protein k... XM_017008432.2 90.6% 100% 1266_1267ins131
27 human 5602 MAPK10 mitogen-activated protein k... XM_024454144.1 90.6% 100% 1266_1267ins131
28 human 5602 MAPK10 mitogen-activated protein k... XM_024454145.1 90.6% 100% 1266_1267ins131
29 human 5602 MAPK10 mitogen-activated protein k... XM_024454146.1 90.6% 100% 1266_1267ins131
30 human 5602 MAPK10 mitogen-activated protein k... XM_024454147.1 90.6% 100% 1266_1267ins131
31 human 5602 MAPK10 mitogen-activated protein k... XM_017008427.2 89.3% 79.9% (many diffs)
32 human 5602 MAPK10 mitogen-activated protein k... XM_017008428.2 89.3% 79.9% (many diffs)
33 human 5602 MAPK10 mitogen-activated protein k... XM_006714269.3 88.5% 98.1% (many diffs)
34 human 5602 MAPK10 mitogen-activated protein k... XM_017008433.2 86.3% 76.2% 0_1ins114;616_617ins72;1063_1064insCAGCA
35 human 5602 MAPK10 mitogen-activated protein k... NM_001351625.2 82.4% 90.9% 0_1ins114;1152_1153ins131
36 human 5602 MAPK10 mitogen-activated protein k... XM_017008434.2 82.4% 90.9% 0_1ins114;1152_1153ins131
37 human 5602 MAPK10 mitogen-activated protein k... XM_017008435.2 82.4% 90.9% 0_1ins114;1152_1153ins131
38 human 5602 MAPK10 mitogen-activated protein k... XM_017008436.2 82.4% 90.9% 0_1ins114;1152_1153ins131
39 human 5602 MAPK10 mitogen-activated protein k... XM_017008437.2 82.4% 90.9% 0_1ins114;1152_1153ins131
40 human 5602 MAPK10 mitogen-activated protein k... NM_001363657.2 80.4% 89% (many diffs)
41 human 5602 MAPK10 mitogen-activated protein k... XM_017008441.2 77.3% 85% 0_1ins114;616_617ins72;1080_1081ins131
42 human 5602 MAPK10 mitogen-activated protein k... XM_017008445.2 75.1% 65.3% 0_1ins342;907_908insCAGCA
43 human 5599 MAPK8 mitogen-activated protein k... NM_001323328.2 71.4% 75.6% (many diffs)
44 human 5599 MAPK8 mitogen-activated protein k... NM_001323329.2 71.4% 75.6% (many diffs)
45 human 5599 MAPK8 mitogen-activated protein k... NM_001323331.2 71.4% 75.6% (many diffs)
46 human 5599 MAPK8 mitogen-activated protein k... NM_139049.4 71.4% 75.6% (many diffs)
47 human 5602 MAPK10 mitogen-activated protein k... XM_017008447.2 68.5% 58.6% 0_1ins435;814_815insCAGCA
48 human 5602 MAPK10 mitogen-activated protein k... XM_017008448.2 68.5% 58.6% 0_1ins435;814_815insCAGCA
49 human 5602 MAPK10 mitogen-activated protein k... NM_001318068.1 66.5% 56.8% (many diffs)
50 human 5602 MAPK10 mitogen-activated protein k... XM_017008449.2 66.5% 56.8% (many diffs)
51 human 5602 MAPK10 mitogen-activated protein k... XM_017008450.2 66.5% 56.8% (many diffs)
52 human 5602 MAPK10 mitogen-activated protein k... XM_024454148.1 66.5% 56.8% (many diffs)
53 human 5602 MAPK10 mitogen-activated protein k... XM_017008451.2 66.1% 72.9% 0_1ins342;924_925ins131
54 human 5599 MAPK8 mitogen-activated protein k... NM_001323302.2 65.8% 84.1% (many diffs)
55 human 5599 MAPK8 mitogen-activated protein k... NM_001323324.2 65.8% 84.1% (many diffs)
56 human 5599 MAPK8 mitogen-activated protein k... NM_001323326.2 65.8% 84.1% (many diffs)
57 human 5599 MAPK8 mitogen-activated protein k... NM_001323327.2 65.8% 84.1% (many diffs)
58 human 5602 MAPK10 mitogen-activated protein k... XR_001741289.1 64.4% (many diffs)
59 human 5599 MAPK8 mitogen-activated protein k... NM_001323321.2 64% 82.9% (many diffs)
60 human 5599 MAPK8 mitogen-activated protein k... NM_001323325.2 64% 82.9% (many diffs)
61 human 5599 MAPK8 mitogen-activated protein k... NM_139046.4 64% 82.9% (many diffs)
62 human 5599 MAPK8 mitogen-activated protein k... XM_024448080.1 64% 82.9% (many diffs)
63 human 5602 MAPK10 mitogen-activated protein k... XM_017008452.2 59.4% 65.6% 0_1ins435;831_832ins131
64 human 5599 MAPK8 mitogen-activated protein k... NM_001323320.2 57.8% 60.8% (many diffs)
65 human 5602 MAPK10 mitogen-activated protein k... XM_017008453.2 53.4% 57.8% (many diffs)
66 human 5602 MAPK10 mitogen-activated protein k... XM_017008454.2 52.3% 57.5% 731_732delGAinsTG;735_735delGins663
67 human 5602 MAPK10 mitogen-activated protein k... XM_024454149.1 52.3% 57.5% 731_732delGAinsTG;735_735delGins663
68 mouse 26414 Mapk10 mitogen-activated protein k... NM_001081567.2 91.4% 89.4% (many diffs)
69 mouse 26414 Mapk10 mitogen-activated protein k... XM_030254516.1 91.4% 89.4% (many diffs)
70 mouse 26414 Mapk10 mitogen-activated protein k... NM_001310685.1 89.4% 87.9% (many diffs)
71 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320886.2 89.4% 87.9% (many diffs)
72 mouse 26414 Mapk10 mitogen-activated protein k... NM_001318102.1 88.7% 89.4% (many diffs)
73 mouse 26414 Mapk10 mitogen-activated protein k... NM_001310683.1 83.4% 81.6% (many diffs)
74 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320888.2 83.4% 81.6% (many diffs)
75 mouse 26414 Mapk10 mitogen-activated protein k... NM_009158.3 82.8% 99.5% (many diffs)
76 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320889.2 81.5% 80.1% (many diffs)
77 mouse 26414 Mapk10 mitogen-activated protein k... NM_001318131.1 80.9% 97.8% (many diffs)
78 mouse 26414 Mapk10 mitogen-activated protein k... XM_006534950.4 80.9% 97.8% (many diffs)
79 mouse 26414 Mapk10 mitogen-activated protein k... XM_011249450.3 76% 72.8% (many diffs)
80 mouse 26414 Mapk10 mitogen-activated protein k... NM_001310686.2 74.8% 90.9% (many diffs)
81 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320890.2 74.8% 90.9% (many diffs)
82 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320891.2 74.8% 90.9% (many diffs)
83 mouse 26414 Mapk10 mitogen-activated protein k... XM_030254518.1 74.2% 71.3% (many diffs)
84 mouse 26414 Mapk10 mitogen-activated protein k... XM_030254519.1 73% 89.3% (many diffs)
85 mouse 26419 Mapk8 mitogen-activated protein k... NM_016700.4 66% 84.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1338
ORF length:
1266
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgagcctc catttcttat actactgcag tgaaccaaca ttggatgtga
121aaattgcctt ttgtcaggga ttcgataaac aagtggatgt gtcatatatt gccaaacatt
181acaacatgag caaaagcaaa gttgacaacc agttctacag tgtggaagtg ggagactcaa
241ccttcacagt tctcaagcgc taccagaatc taaagcctat tggctctggg gctcagggca
301tagtttgtgc cgcgtatgat gctgtccttg acagaaatgt ggccattaag aagctcagca
361gaccctttca gaaccaaaca catgccaaga gagcgtaccg ggagctggtc ctcatgaagt
421gtgtgaacca taaaaacatt attagtttat taaatgtctt cacaccccag aaaacgctgg
481aggagttcca agatgtttac ttagtaatgg aactgatgga tgccaactta tgtcaagtga
541ttcagatgga attagaccat gagcgaatgt cttacctgct gtaccaaatg ttgtgtggca
601ttaagcacct ccattctgct ggaattattc acagggattt aaaaccaagt aacattgtag
661tcaagtctga ttgcacattg aaaatcctgg actttggact ggccaggaca gcaggcacaa
721gcttcatgat gactccatat gtggtgacac gttattacag agcccctgag gtcatcctgg
781ggatgggcta caaggagaac gtggatatat ggtctgtggg atgcattatg ggagaaatgg
841ttcgccacaa aatcctcttt ccaggaaggg actatattga ccagtggaat aaggtaattg
901aacaactagg aacaccatgt ccagaattca tgaagaaatt gcaacccaca gtaagaaact
961atgtggagaa tcggcccaag tatgcgggac tcaccttccc caaactcttc ccagattccc
1021tcttcccagc ggactccgag cacaataaac tcaaagccag ccaagccagg gacttgttgt
1081caaagatgct agtgattgac ccagcaaaaa gaatatcagt ggacgacgcc ttacagcatc
1141cctacatcaa cgtctggtat gacccagccg aagtggaggc gcctccaccT CAGATATATG
1201ACAAGCAGTT GGATGAAAGA GAACACACAA TTGAAGAATG GAAAGAACTT ATCTACAAGG
1261AAGTAATGAA TTCAGAAGAA AAGACTAAAA ATGGTGTAGT AAAAGGACAG CCTTCTCCTT
1321CAGCACAGGT GCAGCAGTGA ACAGCAGTGA GAGTCTCCCT CCATCCTCGT CTGTCAATGA
1381CATCTCCTCC ATGTCCACCG ACCAGACCCT GGCATCTGAC ACTGACAGCA GCCTGGAAGC
1441CTCGGCAGGA CCCCTGGGTT GTTGCAGGTA GGACCCAGCT TTCTTGTACA AAGTGGTTGA
1501TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG
1561TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATGC
1621AGATTATTTT ACTCCTCGAC GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt
1681tgtgaaagat t

Download FASTA (ORF) (Full)

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