Construct: ORF TRCN0000488648

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019101.1_s317c1
DNA Barcode:
ACCAGGGTGAGTGAATCTAAGAAG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
MAPK10 (5602)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488648
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5602 MAPK10 mitogen-activated protein k... NM_138982.4 99.5% 89.8% 1249_1250insCAGCA;1392_1393insG
2 human 5602 MAPK10 mitogen-activated protein k... XM_005263129.3 99.5% 89.8% 1249_1250insCAGCA;1392_1393insG
3 human 5602 MAPK10 mitogen-activated protein k... XM_005263130.3 99.5% 89.8% 1249_1250insCAGCA;1392_1393insG
4 human 5602 MAPK10 mitogen-activated protein k... XM_011532117.3 99.5% 89.8% 1249_1250insCAGCA;1392_1393insG
5 human 5602 MAPK10 mitogen-activated protein k... XM_011532118.3 99.5% 89.8% 1249_1250insCAGCA;1392_1393insG
6 human 5602 MAPK10 mitogen-activated protein k... XM_017008420.2 99.5% 89.8% 1249_1250insCAGCA;1392_1393insG
7 human 5602 MAPK10 mitogen-activated protein k... XM_024454139.1 99.5% 89.8% 1249_1250insCAGCA;1392_1393insG
8 human 5602 MAPK10 mitogen-activated protein k... XM_024454140.1 99.5% 89.8% 1249_1250insCAGCA;1392_1393insG
9 human 5602 MAPK10 mitogen-activated protein k... XM_024454141.1 99.5% 89.8% 1249_1250insCAGCA;1392_1393insG
10 human 5602 MAPK10 mitogen-activated protein k... XM_024454142.1 99.5% 89.8% 1249_1250insCAGCA;1392_1393insG
11 human 5602 MAPK10 mitogen-activated protein k... XM_024454143.1 99.5% 89.8% 1249_1250insCAGCA;1392_1393insG
12 human 5602 MAPK10 mitogen-activated protein k... NM_001318067.1 97.4% 88.1% (many diffs)
13 human 5602 MAPK10 mitogen-activated protein k... XM_005263131.3 97.4% 88.1% (many diffs)
14 human 5602 MAPK10 mitogen-activated protein k... NM_001318069.2 96.7% 89.8% 1249_1250insCAGCA;1393_1434delinsG
15 human 5602 MAPK10 mitogen-activated protein k... XM_017008422.2 94.4% 84.4% 730_731ins72;1177_1178insCAGCA;1320_1321insG
16 human 5602 MAPK10 mitogen-activated protein k... NM_001351624.2 91.4% 81.6% 0_1ins114;1135_1136insCAGCA;1278_1279insG
17 human 5602 MAPK10 mitogen-activated protein k... NM_138980.4 91.4% 81.6% 0_1ins114;1135_1136insCAGCA;1278_1279insG
18 human 5602 MAPK10 mitogen-activated protein k... XM_006714268.3 91.4% 81.6% 0_1ins114;1135_1136insCAGCA;1278_1279insG
19 human 5602 MAPK10 mitogen-activated protein k... XM_011532120.3 91.4% 81.6% 0_1ins114;1135_1136insCAGCA;1278_1279insG
20 human 5602 MAPK10 mitogen-activated protein k... XM_011532121.3 91.4% 81.6% 0_1ins114;1135_1136insCAGCA;1278_1279insG
21 human 5602 MAPK10 mitogen-activated protein k... XM_017008423.2 91.4% 81.6% 0_1ins114;1135_1136insCAGCA;1278_1279insG
22 human 5602 MAPK10 mitogen-activated protein k... NM_002753.5 90.5% 100% 1266_1267ins132
23 human 5602 MAPK10 mitogen-activated protein k... XM_005263135.4 90.5% 100% 1266_1267ins132
24 human 5602 MAPK10 mitogen-activated protein k... XM_017008429.2 90.5% 100% 1266_1267ins132
25 human 5602 MAPK10 mitogen-activated protein k... XM_017008430.2 90.5% 100% 1266_1267ins132
26 human 5602 MAPK10 mitogen-activated protein k... XM_017008432.2 90.5% 100% 1266_1267ins132
27 human 5602 MAPK10 mitogen-activated protein k... XM_024454144.1 90.5% 100% 1266_1267ins132
28 human 5602 MAPK10 mitogen-activated protein k... XM_024454145.1 90.5% 100% 1266_1267ins132
29 human 5602 MAPK10 mitogen-activated protein k... XM_024454146.1 90.5% 100% 1266_1267ins132
30 human 5602 MAPK10 mitogen-activated protein k... XM_024454147.1 90.5% 100% 1266_1267ins132
31 human 5602 MAPK10 mitogen-activated protein k... XM_017008427.2 89.3% 79.9% (many diffs)
32 human 5602 MAPK10 mitogen-activated protein k... XM_017008428.2 89.3% 79.9% (many diffs)
33 human 5602 MAPK10 mitogen-activated protein k... XM_006714269.3 88.4% 98.1% (many diffs)
34 human 5602 MAPK10 mitogen-activated protein k... XM_017008433.2 86.2% 76.2% (many diffs)
35 human 5602 MAPK10 mitogen-activated protein k... NM_001351625.2 82.4% 90.9% 0_1ins114;1152_1153ins132
36 human 5602 MAPK10 mitogen-activated protein k... XM_017008434.2 82.4% 90.9% 0_1ins114;1152_1153ins132
37 human 5602 MAPK10 mitogen-activated protein k... XM_017008435.2 82.4% 90.9% 0_1ins114;1152_1153ins132
38 human 5602 MAPK10 mitogen-activated protein k... XM_017008436.2 82.4% 90.9% 0_1ins114;1152_1153ins132
39 human 5602 MAPK10 mitogen-activated protein k... XM_017008437.2 82.4% 90.9% 0_1ins114;1152_1153ins132
40 human 5602 MAPK10 mitogen-activated protein k... NM_001363657.2 80.3% 89% (many diffs)
41 human 5602 MAPK10 mitogen-activated protein k... XM_017008441.2 77.2% 85% 0_1ins114;616_617ins72;1080_1081ins132
42 human 5602 MAPK10 mitogen-activated protein k... XM_017008445.2 75.1% 65.3% 0_1ins342;907_908insCAGCA;1050_1051insG
43 human 5599 MAPK8 mitogen-activated protein k... NM_001323328.2 71.4% 75.6% (many diffs)
44 human 5599 MAPK8 mitogen-activated protein k... NM_001323329.2 71.4% 75.6% (many diffs)
45 human 5599 MAPK8 mitogen-activated protein k... NM_001323331.2 71.4% 75.6% (many diffs)
46 human 5599 MAPK8 mitogen-activated protein k... NM_139049.4 71.4% 75.6% (many diffs)
47 human 5602 MAPK10 mitogen-activated protein k... XM_017008447.2 68.4% 58.6% 0_1ins435;814_815insCAGCA;957_958insG
48 human 5602 MAPK10 mitogen-activated protein k... XM_017008448.2 68.4% 58.6% 0_1ins435;814_815insCAGCA;957_958insG
49 human 5602 MAPK10 mitogen-activated protein k... NM_001318068.1 66.4% 56.8% (many diffs)
50 human 5602 MAPK10 mitogen-activated protein k... XM_017008449.2 66.4% 56.8% (many diffs)
51 human 5602 MAPK10 mitogen-activated protein k... XM_017008450.2 66.4% 56.8% (many diffs)
52 human 5602 MAPK10 mitogen-activated protein k... XM_024454148.1 66.4% 56.8% (many diffs)
53 human 5602 MAPK10 mitogen-activated protein k... XM_017008451.2 66% 72.9% 0_1ins342;924_925ins132
54 human 5599 MAPK8 mitogen-activated protein k... NM_001323302.2 65.7% 84.1% (many diffs)
55 human 5599 MAPK8 mitogen-activated protein k... NM_001323324.2 65.7% 84.1% (many diffs)
56 human 5599 MAPK8 mitogen-activated protein k... NM_001323326.2 65.7% 84.1% (many diffs)
57 human 5599 MAPK8 mitogen-activated protein k... NM_001323327.2 65.7% 84.1% (many diffs)
58 human 5602 MAPK10 mitogen-activated protein k... XR_001741289.1 64.4% (many diffs)
59 human 5599 MAPK8 mitogen-activated protein k... NM_001323321.2 63.9% 82.9% (many diffs)
60 human 5599 MAPK8 mitogen-activated protein k... NM_001323325.2 63.9% 82.9% (many diffs)
61 human 5599 MAPK8 mitogen-activated protein k... NM_139046.4 63.9% 82.9% (many diffs)
62 human 5599 MAPK8 mitogen-activated protein k... XM_024448080.1 63.9% 82.9% (many diffs)
63 human 5602 MAPK10 mitogen-activated protein k... XM_017008452.2 59.4% 65.6% 0_1ins435;831_832ins132
64 human 5599 MAPK8 mitogen-activated protein k... NM_001323320.2 57.7% 60.8% (many diffs)
65 human 5602 MAPK10 mitogen-activated protein k... XM_017008453.2 53.3% 57.8% (many diffs)
66 human 5602 MAPK10 mitogen-activated protein k... XM_017008454.2 52.3% 57.5% 731_732delGAinsTG;735_735delGins664
67 human 5602 MAPK10 mitogen-activated protein k... XM_024454149.1 52.3% 57.5% 731_732delGAinsTG;735_735delGins664
68 mouse 26414 Mapk10 mitogen-activated protein k... NM_001081567.2 91.3% 89.4% (many diffs)
69 mouse 26414 Mapk10 mitogen-activated protein k... XM_030254516.1 91.3% 89.4% (many diffs)
70 mouse 26414 Mapk10 mitogen-activated protein k... NM_001310685.1 89.3% 87.9% (many diffs)
71 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320886.2 89.3% 87.9% (many diffs)
72 mouse 26414 Mapk10 mitogen-activated protein k... NM_001318102.1 88.7% 89.4% (many diffs)
73 mouse 26414 Mapk10 mitogen-activated protein k... NM_001310683.1 83.4% 81.6% (many diffs)
74 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320888.2 83.4% 81.6% (many diffs)
75 mouse 26414 Mapk10 mitogen-activated protein k... NM_009158.3 82.7% 99.5% (many diffs)
76 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320889.2 81.4% 80.1% (many diffs)
77 mouse 26414 Mapk10 mitogen-activated protein k... NM_001318131.1 80.8% 97.8% (many diffs)
78 mouse 26414 Mapk10 mitogen-activated protein k... XM_006534950.4 80.8% 97.8% (many diffs)
79 mouse 26414 Mapk10 mitogen-activated protein k... XM_011249450.3 76% 72.8% (many diffs)
80 mouse 26414 Mapk10 mitogen-activated protein k... NM_001310686.2 74.8% 90.9% (many diffs)
81 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320890.2 74.8% 90.9% (many diffs)
82 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320891.2 74.8% 90.9% (many diffs)
83 mouse 26414 Mapk10 mitogen-activated protein k... XM_030254518.1 74.1% 71.3% (many diffs)
84 mouse 26414 Mapk10 mitogen-activated protein k... XM_030254519.1 72.9% 89.3% (many diffs)
85 mouse 26419 Mapk8 mitogen-activated protein k... NM_016700.4 66% 84.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1338
ORF length:
1266
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgagcctc catttcttat actactgcag tgaaccaaca ttggatgtga
121aaattgcctt ttgtcaggga ttcgataaac aagtggatgt gtcatatatt gccaaacatt
181acaacatgag caaaagcaaa gttgacaacc agttctacag tgtggaagtg ggagactcaa
241ccttcacagt tctcaagcgc taccagaatc taaagcctat tggctctggg gctcagggca
301tagtttgtgc cgcgtatgat gctgtccttg acagaaatgt ggccattaag aagctcagca
361gaccctttca gaaccaaaca catgccaaga gagcgtaccg ggagctggtc ctcatgaagt
421gtgtgaacca taaaaacatt attagtttat taaatgtctt cacaccccag aaaacgctgg
481aggagttcca agatgtttac ttagtaatgg aactgatgga tgccaactta tgtcaagtga
541ttcagatgga attagaccat gagcgaatgt cttacctgct gtaccaaatg ttgtgtggca
601ttaagcacct ccattctgct ggaattattc acagggattt aaaaccaagt aacattgtag
661tcaagtctga ttgcacattg aaaatcctgg actttggact ggccaggaca gcaggcacaa
721gcttcatgat gactccatat gtggtgacac gttattacag agcccctgag gtcatcctgg
781ggatgggcta caaggagaac gtggatatat ggtctgtggg atgcattatg ggagaaatgg
841ttcgccacaa aatcctcttt ccaggaaggg actatattga ccagtggaat aaggtaattg
901aacaactagg aacaccatgt ccagaattca tgaagaaatt gcaacccaca gtaagaaact
961atgtggagaa tcggcccaag tatgcgggac tcaccttccc caaactcttc ccagattccc
1021tcttcccagc ggactccgag cacaataaac tcaaagccag ccaagccagg gacttgttgt
1081caaagatgct agtgattgac ccagcaaaaa gaatatcagt ggacgacgcc ttacagcatc
1141cctacatcaa cgtctggtat gacccagccg aagtggaggc gcctccaccT CAGATATATG
1201ACAAGCAGTT GGATGAAAGA GAACACACAA TTGAAGAATG GAAAGAACTT ATCTACAAGG
1261AAGTAATGAA TTCAGAAGAA AAGACTAAAA ATGGTGTAGT AAAAGGACAG CCTTCTCCTT
1321CAGCACAGGT GCAGCAGTGA ACAGCAGTGA GAGTCTCCCT CCATCCTCGT CTGTCAATGA
1381CATCTCCTCC ATGTCCACCG ACCAGACCCT GGCATCTGAC ACTGACAGCA GCCTGGAAGC
1441CTCGGCAGGA CCCCTGGGTT GTTGCAGGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT
1501CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC
1561GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACCAGG
1621GTGAGTGAAT CTAAGAAGAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt
1681gaaagatt

Download FASTA (ORF) (Full)