Construct: ORF TRCN0000468758
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012101.1_s317c1
- Derived from:
- ccsbBroadEn_07882
- DNA Barcode:
- TCCCCCAACCTGCTTCACAAAGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRIP1 (23426)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468758
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23426 | GRIP1 | glutamate receptor interact... | NM_021150.4 | 99.9% | 99.9% | 2305C>G |
| 2 | human | 23426 | GRIP1 | glutamate receptor interact... | NM_001178074.2 | 98.5% | 98.5% | 2305C>G;2576_2577ins45 |
| 3 | human | 23426 | GRIP1 | glutamate receptor interact... | NM_001366724.1 | 96.3% | 94.9% | (many diffs) |
| 4 | human | 23426 | GRIP1 | glutamate receptor interact... | NM_001366723.1 | 96.2% | 94.8% | (many diffs) |
| 5 | human | 23426 | GRIP1 | glutamate receptor interact... | NM_001366722.1 | 95.3% | 95.2% | 1043_1198del;2461C>G |
| 6 | human | 23426 | GRIP1 | glutamate receptor interact... | XM_005268754.4 | 93.6% | 93.2% | (many diffs) |
| 7 | human | 23426 | GRIP1 | glutamate receptor interact... | XM_005268757.4 | 92.5% | 90.5% | (many diffs) |
| 8 | human | 23426 | GRIP1 | glutamate receptor interact... | XM_017019099.1 | 92% | 90.6% | (many diffs) |
| 9 | human | 23426 | GRIP1 | glutamate receptor interact... | XM_017019100.1 | 91.5% | 90.2% | (many diffs) |
| 10 | human | 23426 | GRIP1 | glutamate receptor interact... | XM_017019098.1 | 87.5% | 86.2% | (many diffs) |
| 11 | mouse | 74053 | Grip1 | glutamate receptor interact... | NM_001277294.1 | 87.9% | 94.9% | (many diffs) |
| 12 | mouse | 74053 | Grip1 | glutamate receptor interact... | XM_017314126.1 | 87.5% | 94.3% | (many diffs) |
| 13 | mouse | 74053 | Grip1 | glutamate receptor interact... | NM_001277292.1 | 86.1% | 92.9% | (many diffs) |
| 14 | mouse | 74053 | Grip1 | glutamate receptor interact... | NM_130891.2 | 85.5% | 92.4% | (many diffs) |
| 15 | mouse | 74053 | Grip1 | glutamate receptor interact... | NM_133442.2 | 85.2% | 90.1% | (many diffs) |
| 16 | mouse | 74053 | Grip1 | glutamate receptor interact... | NM_028736.2 | 85.1% | 91.8% | (many diffs) |
| 17 | mouse | 74053 | Grip1 | glutamate receptor interact... | XM_006514237.2 | 84.7% | 91.5% | (many diffs) |
| 18 | mouse | 74053 | Grip1 | glutamate receptor interact... | XM_006514236.2 | 84% | 90.2% | (many diffs) |
| 19 | mouse | 74053 | Grip1 | glutamate receptor interact... | XM_006514238.2 | 82.8% | 88.6% | (many diffs) |
| 20 | mouse | 74053 | Grip1 | glutamate receptor interact... | XM_006514242.3 | 81.8% | 86% | (many diffs) |
| 21 | mouse | 74053 | Grip1 | glutamate receptor interact... | XM_006514235.2 | 81.5% | 86.1% | (many diffs) |
| 22 | mouse | 74053 | Grip1 | glutamate receptor interact... | NM_001277293.1 | 81.4% | 87.2% | (many diffs) |
| 23 | mouse | 74053 | Grip1 | glutamate receptor interact... | XM_006514239.2 | 81.3% | 86.4% | (many diffs) |
| 24 | mouse | 74053 | Grip1 | glutamate receptor interact... | XM_006514231.2 | 80.3% | 86.2% | (many diffs) |
| 25 | mouse | 74053 | Grip1 | glutamate receptor interact... | XM_006514232.2 | 80.3% | 86.1% | (many diffs) |
| 26 | mouse | 74053 | Grip1 | glutamate receptor interact... | XM_006514233.2 | 79.1% | 84.9% | (many diffs) |
| 27 | mouse | 74053 | Grip1 | glutamate receptor interact... | XM_006514234.2 | 78.2% | 83.9% | (many diffs) |
| 28 | mouse | 74053 | Grip1 | glutamate receptor interact... | XR_380451.2 | 60.2% | (many diffs) | |
| 29 | mouse | 74053 | Grip1 | glutamate receptor interact... | XR_380450.2 | 60.2% | (many diffs) | |
| 30 | mouse | 74053 | Grip1 | glutamate receptor interact... | NM_001277295.1 | 51.3% | 52.7% | (many diffs) |
| 31 | mouse | 74053 | Grip1 | glutamate receptor interact... | NR_102387.1 | 48.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3297
- ORF length:
- 3228
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatagctgtc tcttttaaat gccgttgtca aattctgagg cgacttacta 121 aagatgagag tccctacact aaatccgcca gccagacaaa gccgcctgat ggagcgttgg 181 ctgtgaggag acagagcatc ccagaggaat tcaagggctc cacagtcgtc gagctgatga 241 agaaggaagg cactaccctg ggtctgacgg tatcgggagg aattgataag gatggcaagc 301 caagagtatc taatctgcgg caaggaggaa ttgctgctag aagtgaccag ctggatgtgg 361 gtgactacat caaagcagtg aatggaatca acctggccaa attccgccat gacgagatca 421 tcagcttgct gaagaatgtg ggagaaagag tggttcttga agtagagtac gagcttccac 481 cggtctctgt gcaaggatca agtgttattt tccgaacagt ggaggtcaca ttacataaag 541 aaggcaatac ctttggtttt gtaattcgag ggggagcaca tgatgataga aataaatctc 601 gtccagttgt gataacatgt gttcgtcctg gagggcctgc tgacagagag ggcacgatca 661 aacccggtga caggttgctc agtgtggatg gaattcggct tcttggaact acgcatgctg 721 aagccatgag tattcttaaa caatgtggac aagaagcagc actgctgata gaatatgatg 781 tctcagtaat ggactctgtg gcaacagcat ccgggccact actagtcgaa gttgccaaaa 841 ctcctggtgc cagccttggg gttgccctaa ctacctcgat gtgctgtaac aaacaagtca 901 ttgtcataga caaaatcaaa tctgcaagta ttgcagacag atgtggcgca ttgcatgtgg 961 gagatcacat cctctccatc gatggaacca gcatggagta ctgtacactt gcagaagcaa 1021 cccagttcct ggccaacacc actgaccagg tcaagcttga gatccttccc catcatcaga 1081 cccggctggc cctaaagggg cccgaccatg cagctttggt gtcttcatcc ttctctccta 1141 cctccatgag tgcatacagc ctgagttccc tgaacatggg gactctacct cgaagcctct 1201 actccaccag cccacgtgga accatgatga ggaggagact gaaaaagaaa gacttcaaaa 1261 gctcattgtc cttagcctcc agcacagtag gattggctgg gcaggttgtt cacacagaaa 1321 ccacagaggt tgtgctgacg gcagatcctg tcacaggatt tgggatccaa ctgcagggca 1381 gtgtgtttgc cacagaaact ctctcttctc cacctctgat ttcctatatc gaagctgaca 1441 gcccagcaga gagatgtggg gtgctacaga ttggagacag agtgatggcc atcaatggaa 1501 ttccaacaga agacagcacc ttcgaagaag ccagtcagct cctccgagac tcttcaatca 1561 cgagcaaggt cacactggaa atcgagtttg atgttgcaga gtctgtcatc ccaagtagtg 1621 gaacatttca tgtaaagctg cctaagaagc acaatgtgga acttggaata accataagtt 1681 caccatccag tagaaaacca ggagaccccc tcgtcatttc agatatcaag aaagggagtg 1741 tggcacacag aactgggacc ctggaacttg gggataaatt gctcgcaata gataatatcc 1801 ggctggacaa ctgttccatg gaagatgcag ttcagatcct ccagcaatgt gaagacctgg 1861 tgaagctcaa aatccgcaaa gatgaagata attcagatga gcaagaaagt tccggagcaa 1921 ttatttacac cgtggagctt aaacgctacg gggggcccct tggcatcaca atttcaggaa 1981 ctgaagagcc gtttgatcct ataatcattt caagcctcac taaaggggga ttagctgaaa 2041 gaactggcgc aatccacata ggagaccgaa tcctagccat caatagcagc agcttgaaag 2101 ggaagcctct gagtgaagcc atccatttgt tacagatggc aggagagact gtcaccttga 2161 aaattaagaa acagacagat gcccagtcag catcgagccc caagaagttc cctatttcta 2221 gccatttgag tgacctgggg gatgtggagg aggactcctc accagcacag aagccaggca 2281 agctctccga catgtacccc tccacggtgc ccagtgtgga cagtgctgtg gattcatggg 2341 atgggtctgc aatagacacc agctatggaa ctgaaggcac tagttttcag gcctcaggat 2401 acaatttcaa cacctatgac tggaggagtc caaaacagag aggcagcttg tccccagtca 2461 ctaagcctcg aagccagact tacccagatg tggggctgag ttatgaagac tgggaccggt 2521 ccacagccag tggttttgca ggggctgccg atagtgcaga gacagaacaa gaggagaact 2581 tctggtctca agcgctggag gatttggaaa cctgcggaca gtcaggaatt ctgagagaac 2641 tggaggagaa agctgacagg cgtgtgtctt tgagaaacat gactctcttg gcaacaatca 2701 tgtcggggag cacgatgagt ttgaatcatg aggctccaac acctcgcagt cagctggggc 2761 gacaggccag cttccaggag cgcagcagct cgcggccgca ctacagccaa acaactcgga 2821 gcaacaccct gccttcagat gtgggtagga agtcagtaac ccTGAGAAAA ATGAAACAAG 2881 AAATAAAGGA GATCATGTCT CCAACTCCTG TGGAGCTGCA CAAGGTGACC TTGTACAAGG 2941 ACTCTGACAT GGAGGACTTT GGGTTCAGTG TAGCAGATGG CTTACTGGAG AAAGGAGTGT 3001 ATGTCAAAAA TATTCGCCCA GCTGGGCCAG GAGATCTTGG TGGCTTAAAG CCCTATGACA 3061 GGCTCTTACA GGTGAATCAT GTCCGAACCA GAGACTTTGA CTGCTGCCTT GTTGTGCCCC 3121 TCATAGCAGA ATCCGGGAAT AAGCTGGACC TGGTTATTAG TAGAAACCCA CTGGCTTCAC 3181 AGAAGTCTAT AGACCAACAG AGTCTACCAG GAGATTGGAG TGAACAGAAC AGTGCTTTTT 3241 TCCAGCAGCC TAGCCACGGT GGTAATTTGG AGACACGAGA ACCCACTAAT ACATTATTGC 3301 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 3361 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 3421 ATATATCTTG TGGAAAGGAC GATCCCCCAA CCTGCTTCAC AAAGACACGC GTTAAGTCga 3481 caatcaacct ctggattaca aaatttgtga aagatt