Construct: ORF TRCN0000474301

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005146.1_s317c1
Derived from:
ccsbBroadEn_10171
DNA Barcode:
GGCATGATAACGCGACACGTACAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
STAG3L3 (442578)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474301
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 378108 TRIM74 tripartite motif containing 74 XM_017012197.2 68.7% 61% (many diffs)
2 human 378108 TRIM74 tripartite motif containing 74 XR_001744733.2 37.6% (many diffs)
3 human 378108 TRIM74 tripartite motif containing 74 XR_001744745.2 36.6% (many diffs)
4 human 378108 TRIM74 tripartite motif containing 74 XR_001744740.2 35.4% 1_392del;795_1135del
5 human 378108 TRIM74 tripartite motif containing 74 XR_001744744.2 28.6% 1_312del;715_1402del
6 human 378108 TRIM74 tripartite motif containing 74 XR_002956448.1 25.2% 1_363del;711_847del;903_1590del
7 human 378108 TRIM74 tripartite motif containing 74 XR_002956445.1 24.3% (many diffs)
8 human 378108 TRIM74 tripartite motif containing 74 XR_001744741.2 22.2% 1_392del;795_1806del
9 human 378108 TRIM74 tripartite motif containing 74 XR_001744734.2 20.9% (many diffs)
10 human 378108 TRIM74 tripartite motif containing 74 XR_002956442.1 19.2% (many diffs)
11 human 54441 STAG3L1 stromal antigen 3-like 1 (p... NR_040583.2 18.8% 1_508del;911_2138del
12 human 442578 STAG3L3 stromal antigen 3-like 3 (p... NR_040582.2 18.5% 1_527del;930_2167del
13 human 378108 TRIM74 tripartite motif containing 74 XR_002956438.1 17.8% (many diffs)
14 human 378108 TRIM74 tripartite motif containing 74 XR_002956441.1 17.5% (many diffs)
15 human 378108 TRIM74 tripartite motif containing 74 XR_002956440.1 17% (many diffs)
16 human 442582 STAG3L2 stromal antigen 3-like 2 (p... NR_040584.2 16.9% (many diffs)
17 human 378108 TRIM74 tripartite motif containing 74 XR_001744739.2 16.7% 1_392del;795_2404del
18 human 378108 TRIM74 tripartite motif containing 74 XR_001744737.2 16.4% 1_392del;740_779del;835_2444del
19 human 378108 TRIM74 tripartite motif containing 74 XR_001744732.2 15.7% (many diffs)
20 human 378108 TRIM74 tripartite motif containing 74 XR_001744742.1 14.4% 1_392del;795_2774del
21 human 378108 TRIM74 tripartite motif containing 74 XR_001744751.2 12% 1_363del;405_406ins124;642_2176del
22 human 378108 TRIM74 tripartite motif containing 74 XR_002956439.1 11.9% (many diffs)
23 human 378108 TRIM74 tripartite motif containing 74 XR_001744750.1 11.7% 1_363del;405_406ins124;642_2251del
24 human 378108 TRIM74 tripartite motif containing 74 XR_001744731.2 11.6% (many diffs)
25 human 378108 TRIM74 tripartite motif containing 74 XR_001744735.1 11.3% (many diffs)
26 human 378108 TRIM74 tripartite motif containing 74 XR_001744736.1 10.9% (many diffs)
27 human 10734 STAG3 stromal antigen 3 NM_001282718.2 10.2% 9.6% (many diffs)
28 human 10734 STAG3 stromal antigen 3 XM_017011687.1 10.1% 9.5% (many diffs)
29 human 378108 TRIM74 tripartite motif containing 74 XR_002956443.1 10% 1_392del;434_435ins124;671_2650del
30 human 10734 STAG3 stromal antigen 3 NM_001282716.1 9.7% 9.2% (many diffs)
31 human 10734 STAG3 stromal antigen 3 NM_012447.4 9.7% 9.2% (many diffs)
32 human 10734 STAG3 stromal antigen 3 NM_001282717.1 9.7% 9.2% (many diffs)
33 human 10734 STAG3 stromal antigen 3 XM_011515742.1 9.7% 9.2% (many diffs)
34 human 10734 STAG3 stromal antigen 3 XM_017011686.2 9.6% 9.1% (many diffs)
35 human 10734 STAG3 stromal antigen 3 XM_017011683.2 9.6% 9.1% (many diffs)
36 human 10734 STAG3 stromal antigen 3 XM_017011684.1 9.6% 9.1% (many diffs)
37 human 10734 STAG3 stromal antigen 3 XM_017011685.1 9.6% 9.1% (many diffs)
38 human 378108 TRIM74 tripartite motif containing 74 XR_002956449.1 8.5% (many diffs)
39 human 378108 TRIM74 tripartite motif containing 74 XR_002956444.1 6.6% 1_281del;323_324ins124;560_4069del
40 human 378108 TRIM74 tripartite motif containing 74 XR_002956447.1 6.5% (many diffs)
41 human 378108 TRIM74 tripartite motif containing 74 XR_002956450.1 6.5% (many diffs)
42 human 378108 TRIM74 tripartite motif containing 74 XR_001744749.2 6.4% 1_392del;434_435ins124;671_4180del
43 human 378108 TRIM74 tripartite motif containing 74 XR_002956446.1 6.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
468
ORF length:
402
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgat cttttcaatg ctgcgaaagc tgccaaaagt gacatgcagg gatgtccttc
121ctgagatccg tgctatctgc attgaggaaa ttgggtgttg gatgcaaagc tacagcacgt
181ctttcctcac cgacagctat ttaaaatata ttggttggac tcTGCATGAT AAGCACCGAG
241AAGTCCGCGT GAAGTGCGTG AAGGCTCTGA AAGGGCTGTA CGGTAACCGG GACCTGACCG
301CACGCCTGGA GCTCTTCACT GGCCGCTTCA AGGACTGGAT GGTTTCCATG ATCATGGACA
361GAGAGTACAG TGTGGCAGTG GAGGCCGTCA GATTACTGAT ACTTATCCTT AAGAACATGG
421AAGGGGTGCT GATGGACGTG GACTGTGAGA GCGTCTACCC CATTGTGTAC CCAACTTTCT
481TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
541CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
601GTGGAAAGGA CGAGGCATGA TAACGCGACA CGTACACACG CGTTAAGTCg acaatcaacc
661tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)