Construct: ORF TRCN0000474301
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005146.1_s317c1
- Derived from:
- ccsbBroadEn_10171
- DNA Barcode:
- GGCATGATAACGCGACACGTACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STAG3L3 (442578)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474301
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 378108 | TRIM74 | tripartite motif containing 74 | XM_017012197.2 | 68.7% | 61% | (many diffs) |
| 2 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744733.2 | 37.6% | (many diffs) | |
| 3 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744745.2 | 36.6% | (many diffs) | |
| 4 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744740.2 | 35.4% | 1_392del;795_1135del | |
| 5 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744744.2 | 28.6% | 1_312del;715_1402del | |
| 6 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_002956448.1 | 25.2% | 1_363del;711_847del;903_1590del | |
| 7 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_002956445.1 | 24.3% | (many diffs) | |
| 8 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744741.2 | 22.2% | 1_392del;795_1806del | |
| 9 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744734.2 | 20.9% | (many diffs) | |
| 10 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_002956442.1 | 19.2% | (many diffs) | |
| 11 | human | 54441 | STAG3L1 | stromal antigen 3-like 1 (p... | NR_040583.2 | 18.8% | 1_508del;911_2138del | |
| 12 | human | 442578 | STAG3L3 | stromal antigen 3-like 3 (p... | NR_040582.2 | 18.5% | 1_527del;930_2167del | |
| 13 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_002956438.1 | 17.8% | (many diffs) | |
| 14 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_002956441.1 | 17.5% | (many diffs) | |
| 15 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_002956440.1 | 17% | (many diffs) | |
| 16 | human | 442582 | STAG3L2 | stromal antigen 3-like 2 (p... | NR_040584.2 | 16.9% | (many diffs) | |
| 17 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744739.2 | 16.7% | 1_392del;795_2404del | |
| 18 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744737.2 | 16.4% | 1_392del;740_779del;835_2444del | |
| 19 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744732.2 | 15.7% | (many diffs) | |
| 20 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744742.1 | 14.4% | 1_392del;795_2774del | |
| 21 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744751.2 | 12% | 1_363del;405_406ins124;642_2176del | |
| 22 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_002956439.1 | 11.9% | (many diffs) | |
| 23 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744750.1 | 11.7% | 1_363del;405_406ins124;642_2251del | |
| 24 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744731.2 | 11.6% | (many diffs) | |
| 25 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744735.1 | 11.3% | (many diffs) | |
| 26 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744736.1 | 10.9% | (many diffs) | |
| 27 | human | 10734 | STAG3 | stromal antigen 3 | NM_001282718.2 | 10.2% | 9.6% | (many diffs) |
| 28 | human | 10734 | STAG3 | stromal antigen 3 | XM_017011687.1 | 10.1% | 9.5% | (many diffs) |
| 29 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_002956443.1 | 10% | 1_392del;434_435ins124;671_2650del | |
| 30 | human | 10734 | STAG3 | stromal antigen 3 | NM_001282716.1 | 9.7% | 9.2% | (many diffs) |
| 31 | human | 10734 | STAG3 | stromal antigen 3 | NM_012447.4 | 9.7% | 9.2% | (many diffs) |
| 32 | human | 10734 | STAG3 | stromal antigen 3 | NM_001282717.1 | 9.7% | 9.2% | (many diffs) |
| 33 | human | 10734 | STAG3 | stromal antigen 3 | XM_011515742.1 | 9.7% | 9.2% | (many diffs) |
| 34 | human | 10734 | STAG3 | stromal antigen 3 | XM_017011686.2 | 9.6% | 9.1% | (many diffs) |
| 35 | human | 10734 | STAG3 | stromal antigen 3 | XM_017011683.2 | 9.6% | 9.1% | (many diffs) |
| 36 | human | 10734 | STAG3 | stromal antigen 3 | XM_017011684.1 | 9.6% | 9.1% | (many diffs) |
| 37 | human | 10734 | STAG3 | stromal antigen 3 | XM_017011685.1 | 9.6% | 9.1% | (many diffs) |
| 38 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_002956449.1 | 8.5% | (many diffs) | |
| 39 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_002956444.1 | 6.6% | 1_281del;323_324ins124;560_4069del | |
| 40 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_002956447.1 | 6.5% | (many diffs) | |
| 41 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_002956450.1 | 6.5% | (many diffs) | |
| 42 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744749.2 | 6.4% | 1_392del;434_435ins124;671_4180del | |
| 43 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_002956446.1 | 6.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 468
- ORF length:
- 402
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat cttttcaatg ctgcgaaagc tgccaaaagt gacatgcagg gatgtccttc 121 ctgagatccg tgctatctgc attgaggaaa ttgggtgttg gatgcaaagc tacagcacgt 181 ctttcctcac cgacagctat ttaaaatata ttggttggac tcTGCATGAT AAGCACCGAG 241 AAGTCCGCGT GAAGTGCGTG AAGGCTCTGA AAGGGCTGTA CGGTAACCGG GACCTGACCG 301 CACGCCTGGA GCTCTTCACT GGCCGCTTCA AGGACTGGAT GGTTTCCATG ATCATGGACA 361 GAGAGTACAG TGTGGCAGTG GAGGCCGTCA GATTACTGAT ACTTATCCTT AAGAACATGG 421 AAGGGGTGCT GATGGACGTG GACTGTGAGA GCGTCTACCC CATTGTGTAC CCAACTTTCT 481 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 541 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 601 GTGGAAAGGA CGAGGCATGA TAACGCGACA CGTACACACG CGTTAAGTCg acaatcaacc 661 tctggattac aaaatttgtg aaagatt