Construct: ORF ccsbBroad304_05291

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012648.1_s304c1, BRDN0000408234
Derived from:
ccsbBroadEn_05291
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EBF3 (253738)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05291
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 253738 EBF3 EBF transcription factor 3 NM_001005463.3 100% 100%
2 human 253738 EBF3 EBF transcription factor 3 XM_006717743.3 98.3% 98.3% 754_780del
3 human 253738 EBF3 EBF transcription factor 3 XM_005252669.3 93.8% 93.6% 1535_1642del
4 human 253738 EBF3 EBF transcription factor 3 XM_006717742.3 92.5% 90.9% (many diffs)
5 human 253738 EBF3 EBF transcription factor 3 XM_005252668.3 92.4% 92.2% 754_780del;1562_1669del
6 human 253738 EBF3 EBF transcription factor 3 XM_006717741.3 90.9% 90.6% (many diffs)
7 human 253738 EBF3 EBF transcription factor 3 XM_005252667.3 88.5% 86.8% (many diffs)
8 human 253738 EBF3 EBF transcription factor 3 XM_006717740.3 87.3% 85.8% (many diffs)
9 human 253738 EBF3 EBF transcription factor 3 XM_006717739.3 87.2% 85.6% (many diffs)
10 human 253738 EBF3 EBF transcription factor 3 XM_017016027.1 87.1% 87.1% 754_780del;1372_1373ins189
11 human 253738 EBF3 EBF transcription factor 3 XM_006717744.3 81.9% 80.3% (many diffs)
12 human 253738 EBF3 EBF transcription factor 3 XM_011539574.2 74.6% 73% (many diffs)
13 human 253738 EBF3 EBF transcription factor 3 XM_011539575.2 59.1% 56.2% (many diffs)
14 human 1879 EBF1 EBF transcription factor 1 NM_001364158.1 55.4% 64.5% (many diffs)
15 human 1879 EBF1 EBF transcription factor 1 NM_001364159.1 54.6% 63.6% (many diffs)
16 human 1879 EBF1 EBF transcription factor 1 NM_001324111.2 54.5% 63.5% (many diffs)
17 human 1879 EBF1 EBF transcription factor 1 XM_024454393.1 54.5% 63.5% (many diffs)
18 human 253738 EBF3 EBF transcription factor 3 XR_001747076.1 32.5% 1_506del;2128_2345del;2378_5076del
19 mouse 13593 Ebf3 early B cell factor 3 NM_010096.3 94.3% 99.6% (many diffs)
20 mouse 13593 Ebf3 early B cell factor 3 XM_006507335.1 92.7% 98% (many diffs)
21 mouse 13593 Ebf3 early B cell factor 3 NM_001113414.1 88.5% 93.3% (many diffs)
22 mouse 13593 Ebf3 early B cell factor 3 NM_001113415.1 87.1% 91.9% (many diffs)
23 mouse 13593 Ebf3 early B cell factor 3 XM_011241672.1 86.8% 90% (many diffs)
24 mouse 13593 Ebf3 early B cell factor 3 XM_006507333.1 86.8% 90.6% (many diffs)
25 mouse 13593 Ebf3 early B cell factor 3 XM_006507332.1 83% 86.5% (many diffs)
26 mouse 13593 Ebf3 early B cell factor 3 XM_006507331.1 82% 85.5% (many diffs)
27 mouse 13593 Ebf3 early B cell factor 3 XM_006507329.3 81.8% 85.2% (many diffs)
28 mouse 13593 Ebf3 early B cell factor 3 XM_006507330.3 81.8% 85.2% (many diffs)
29 mouse 13593 Ebf3 early B cell factor 3 XM_006507334.1 79.9% 81.7% (many diffs)
30 mouse 13593 Ebf3 early B cell factor 3 XM_006507337.3 77% 78.9% (many diffs)
31 mouse 13593 Ebf3 early B cell factor 3 XM_006507336.1 76.3% 80.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1722
ORF length:
1653
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gtttgggatt caggagaata ttccgcgcgg ggggacgacc atgaaggagg
121agccgctggg cagcggcatg aacccggtgc gctcgtggat gcacacggcg ggcgtggtgg
181acgccaacac ggccgcccag agcggcgtgg ggctggcgcg ggcgcacttc gagaagcagc
241cgccttccaa cctccggaaa tccaatttct tccacttcgt gctggcgctc tacgataggc
301aggggcagcc ggtggagatt gaaaggaccg cttttgtgga ctttgtggag aaagagaaag
361agccaaacaa cgagaaaacc aacaacggca tccactataa actccagtta ttgtacagca
421acggagtcag aacagagcaa gatctgtatg ttcgcctcat agattcaatg accaaacagg
481ccatcgtcta cgagggccag gacaagaacc cggagatgtg ccgtgtgctg ctgacccacg
541agatcatgtg cagccggtgc tgtgacaaga aaagttgtgg caatagaaac gaaacgccct
601cagaccctgt aatcattgac agattctttc taaagttttt cctcaagtgc aatcagaact
661gtttgaagaa tgcaggcaac cctcgagata tgcggagatt ccaggttgtt gtatcgacaa
721cagtcaacgt ggacggccac gtgctggccg tgtcagacaa catgtttgtg cacaacaatt
781ccaaacacgg gaggcgggcc cgccgcctag acccgtcaga agccactccg tgcatcaagg
841ccatcagtcc cagtgaaggc tggaccacgg ggggtgccac cgtcatcata attggcgaca
901acttctttga cgggctgcaa gttgtattcg gaactatgtt ggtgtggagc gagctgataa
961ctccccatgc catccgagtc cagaccccgc cgaggcacat tcctggcgtc gtcgaagtga
1021ccctctccta caaatccaag cagttctgca aaggtgctcc tgggcgcttt gtctacaccg
1081cccttaatga accaaccata gattacggct ttcagaggtt gcagaaagtg atcccaagac
1141atccgggtga tcccgaaagg ttacccaagg aggtgttact gaagcgggcg gcggacctgg
1201tggaagcctt atacggaatg cctcacaaca accaggagat catcttgaag cgagcggcgg
1261acatcgccga ggcgctgtac agcgttcccc gcaatcacaa ccagatcccc accctgggca
1321acaaccctgc acacacgggc atgatgggcg tcaactcctt cagcagccag ctagccgtca
1381acgtgtcaga gacgtcacaa gccaacgacc aagtcggcta cagtcgcaat acaagcagcg
1441tgtccccgcg aggctacgtc cccagcagta ctccccagca gtccaattac aacacagtca
1501gcactagcat gaatggatat ggaagtggcg ccatggccag tctaggggtc cctggctcgc
1561ctggatttct taatggctcc tccgctaact ctccctacgg catgaaacag aagagcgcct
1621tcgcgcccgt ggtccggccc caagcctctc ctcctccttc ctgcaccagc gccaacggga
1681atggactgca agctatgtct gggctggtag tcccgccaat gttgccaact ctcttgtaca
1741aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1801ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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