Construct: ORF ccsbBroad304_07959
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011283.1_s304c1, BRDN0000408130
- Derived from:
- ccsbBroadEn_07959
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- INTS7 (25896)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07959
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25896 | INTS7 | integrator complex subunit 7 | NM_015434.4 | 99.9% | 99.8% | 1274A>G |
| 2 | human | 25896 | INTS7 | integrator complex subunit 7 | NM_001199811.1 | 98.5% | 98.4% | 1274A>G;2273_2274ins42 |
| 3 | human | 25896 | INTS7 | integrator complex subunit 7 | NM_001199812.1 | 97.8% | 97.8% | 1274A>G;2315_2316ins60 |
| 4 | human | 25896 | INTS7 | integrator complex subunit 7 | XM_011509396.2 | 96.3% | 96.2% | 1274A>G;2314_2421del |
| 5 | human | 25896 | INTS7 | integrator complex subunit 7 | NM_001199809.1 | 94.8% | 94.8% | 223_224ins147;1127A>G |
| 6 | human | 25896 | INTS7 | integrator complex subunit 7 | NR_037667.1 | 62.8% | (many diffs) | |
| 7 | human | 25896 | INTS7 | integrator complex subunit 7 | XM_017000962.1 | 53.1% | 50.9% | (many diffs) |
| 8 | mouse | 77065 | Ints7 | integrator complex subunit 7 | NM_178632.6 | 85.9% | 94.8% | (many diffs) |
| 9 | mouse | 77065 | Ints7 | integrator complex subunit 7 | NM_001306203.1 | 74.8% | 82.1% | (many diffs) |
| 10 | mouse | 77065 | Ints7 | integrator complex subunit 7 | NM_001306204.1 | 74.8% | 82.1% | (many diffs) |
| 11 | mouse | 77065 | Ints7 | integrator complex subunit 7 | XR_865812.3 | 48.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2952
- ORF length:
- 2886
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 tggtcatggc gtcaaactca actaagtctt tcctggcaga tgccggctat ggcgaacagg 121 aactggatgc caactctgcc cttatggaat tggacaaagg cctaagatct ggcaaacttg 181 gtgaacagtg tgaagcagtt gttcgctttc ccagactttt tcagaagtat ccattcccta 241 ttcttatcaa ttctgcattc ctaaagttag ctgatgtttt cagagttgga aataatttcc 301 tgaggctatg tgttcttaaa gttacccaac aaagtgagaa acatttggag aagattctaa 361 atgtggatga atttgtgaag agaatttttt ctgtgattca tagtaatgat cctgtggcaa 421 gagccatcac cctccggatg ttgggaagtc tggcatcaat aattcctgag aggaagaatg 481 ctcatcatag tattcgtcag agtttagatt cacatgataa tgtagaagtt gaagctgctg 541 tttttgctgc tgcaaacttc tctgcacagt caaaggattt tgctgtagga atctgtaaca 601 aaatcagtga aatgattcaa ggtttagcga caccagtaga cttgaagcta aaattgatac 661 ccattctaca gcacatgcac catgatgcaa tcttggcttc cagtgctcgt cagcttttac 721 aacagctggt cacatcctat ccgtccacca aaatggtgat tgtgtctttg cacactttca 781 ctctgcttgc agcgtcatct ttggttgata cacctaagca gattcagctt ctgttgcagt 841 atttgaagaa tgatcccagg aaggcagtaa agagacttgc tattcaagat ctgaaattac 901 ttgctaataa aacaccacat acttggagta gggagaatat tcaggcactt tgtgagtgtg 961 ccctccagac tccttatgac agcttaaaac tagggatgtt gtctgtcctt tccacactat 1021 cagggaccat cgccatcaaa cattacttca gtatagttcc aggaaatgtg agttcttctc 1081 ccagatcttc tgatttagtc aaattagccc aagagtgctg ttaccataat aacaggggca 1141 ttgcagctca tggagttaga gtcctaacta atataactgt ttcttgtcaa gaaaaggatc 1201 ttttggcact ggaacaagat gctgtctttg gcctggaatc cctactggta ctttgtagtc 1261 aagatgatag tccaggtgct caggccactt taaagattgc tctaaactgt atggtgaagt 1321 tggccaaggg caggccccgt cttagccagt cagtagttga gaccttgttg actcaattgc 1381 acagtgctca agacgctgcc cggattttga tgtgccattg cctggcagcc attgccatgc 1441 aactgccggt gctgggtgat gggatgcttg gtgacctcat ggagctgtac aaggtgattg 1501 gacgatcagc cacagacaag caacaagaac ttctggtgag tttggctact gtgatttttg 1561 ttgcaagtca gaaggcattg tctgtggaaa gtaaggcagt aattaagcag cagcttgaaa 1621 gtgtctccaa tggatggact gtataccgta ttgccagaca ggcttccaga atgggtaatc 1681 atgacatggc caaagagctt tatcagagtt tgctgactca ggttgcctca gaacatttct 1741 acttctggct aaatagtttg aaggagtttt cacatgcaga acagtgtctc actgggttgc 1801 aagaggaaaa ttatagttca gcactttctt gcattgctga atctttaaaa ttctatcaca 1861 aagggattgc ttccttaaca gcagctagta caccactgaa tcctttaagc tttcagtgtg 1921 aatttgtaaa actcaggatt gaccttttac aagccttctc tcaacttatc tgtacttgta 1981 atagcctgaa gacaagccca ccacctgcaa ttgccacaac aattgccatg accttaggaa 2041 atgacctcca gaggtgtggt cgcatctcca atcagatgaa acagtccatg gaagaatttc 2101 gaagccttgc ttctcgatat ggagatcttt accaggcatc ttttgatgct gactcagcaa 2161 ctttgaggaa tgttgaacta cagcagcaga gctgtttact gatatctcat gcaatagaag 2221 ccctgatttt ggatccagaa tcagcaagtt tccaggaata tggatctact ggaacagccc 2281 atgctgatag tgaatatgaa agaagaatga tgtctgtata taatcatgtc ttggaggagg 2341 tagaatcact caatcggaaa tatacccctg tttcttatat gcacacagca tgcctctgca 2401 atgccatcat tgctttgctg aaagttcccc tttctttcca gagatatttt ttccagaaac 2461 tacagtctac cagcatcaag cttgctctgt caccatcgcc ccggaatcct gcagagccca 2521 ttgctgtcca gaataaccag cagctggcgc taaaggtaga gggagtggtt cagcacggat 2581 ctaaaccagg actcttccgc aaaattcagt ctgtctgtct gaatgtttct tccacactgc 2641 agagtaaatc tggacaagac tacaagatac ccattgacaa catgaccaat gagatggagc 2701 aaagggttga acctcataat gattacttca gtactcaatt tctgttgaac tttgctatcc 2761 ttggaacaca caacattaca gtggaatctt ctgtgaaaga tgccaatggt atagtatgga 2821 agactggtcc cagaactacc atatttgtaa aatccctgga agacccttat tcccagcaaa 2881 ttcgcttaca acagcagcaa gcccagcagc cattacagca gcagcagcaa cgcaatgcct 2941 acacacggtt ttaaccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 3001 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 3061 caatcaacct ctggatta