Construct: ORF TRCN0000467633

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000728.1_s317c1
Derived from:
ccsbBroadEn_08191
DNA Barcode:
TGAACTCCATTTGGGGCGGGAACC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NTM (50863)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467633
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 50863 NTM neurotrimin NM_001144059.1 99.8% 100% 577A>C
2 human 50863 NTM neurotrimin XM_011542861.2 96.5% 96% (many diffs)
3 human 50863 NTM neurotrimin NM_016522.2 91% 90.6% (many diffs)
4 human 50863 NTM neurotrimin XM_017017865.2 90.8% 87% (many diffs)
5 human 50863 NTM neurotrimin NM_001144058.1 88.5% 88.1% (many diffs)
6 human 50863 NTM neurotrimin NM_001352004.1 88.3% 87.9% (many diffs)
7 human 50863 NTM neurotrimin NM_001048209.2 85.7% 82.3% (many diffs)
8 human 50863 NTM neurotrimin NM_001352002.1 85.7% 85.2% (many diffs)
9 human 50863 NTM neurotrimin NM_001352007.2 84.5% 83% (many diffs)
10 human 50863 NTM neurotrimin XM_017017862.2 84.5% 83% (many diffs)
11 human 50863 NTM neurotrimin XM_024448568.1 84.5% 83% (many diffs)
12 human 50863 NTM neurotrimin XM_017017854.1 84.2% 83.9% (many diffs)
13 human 50863 NTM neurotrimin XM_017017866.2 83.9% 83.4% (many diffs)
14 human 50863 NTM neurotrimin NM_001352005.2 83.5% 80.1% (many diffs)
15 human 50863 NTM neurotrimin NM_001352003.2 83.3% 79.8% (many diffs)
16 human 50863 NTM neurotrimin NM_001352008.1 82.9% 82.4% (many diffs)
17 human 50863 NTM neurotrimin NM_001352006.2 82.3% 80.8% (many diffs)
18 human 50863 NTM neurotrimin XM_017017858.2 82.3% 80.8% (many diffs)
19 human 50863 NTM neurotrimin XM_017017859.1 82.3% 80.8% (many diffs)
20 human 50863 NTM neurotrimin NM_001352001.2 80.9% 77.5% (many diffs)
21 human 50863 NTM neurotrimin XM_017017856.1 79.7% 78.2% (many diffs)
22 human 50863 NTM neurotrimin XM_017017857.2 79.7% 78.2% (many diffs)
23 human 50863 NTM neurotrimin XM_017017855.2 79.5% 76.3% (many diffs)
24 human 50863 NTM neurotrimin NM_001352009.2 79.1% 78.7% (many diffs)
25 human 50863 NTM neurotrimin XM_017017852.2 77.2% 73.9% (many diffs)
26 human 50863 NTM neurotrimin XM_024448569.1 76.9% 76.6% (many diffs)
27 human 50863 NTM neurotrimin XM_024448566.1 76% 74.6% (many diffs)
28 human 50863 NTM neurotrimin XM_024448567.1 74.5% 74% (many diffs)
29 human 50863 NTM neurotrimin XM_017017863.1 73.2% 72.9% (many diffs)
30 human 50863 NTM neurotrimin XM_017017860.2 71% 70.6% (many diffs)
31 human 50863 NTM neurotrimin XR_001747896.1 58% (many diffs)
32 human 50863 NTM neurotrimin NR_147850.2 31.1% (many diffs)
33 human 50863 NTM neurotrimin NR_147848.2 29.7% (many diffs)
34 human 50863 NTM neurotrimin NR_147853.1 26.9% (many diffs)
35 human 50863 NTM neurotrimin NR_147851.1 26.7% (many diffs)
36 human 50863 NTM neurotrimin XR_001747893.1 24.3% (many diffs)
37 human 50863 NTM neurotrimin NR_147849.2 23.9% (many diffs)
38 human 50863 NTM neurotrimin NR_147854.1 20.9% (many diffs)
39 human 50863 NTM neurotrimin NR_147852.1 20.1% (many diffs)
40 human 50863 NTM neurotrimin XR_001747894.2 16.1% (many diffs)
41 mouse 235106 Ntm neurotrimin XM_006510208.3 81.2% 87% (many diffs)
42 mouse 235106 Ntm neurotrimin XM_006510207.3 80.9% 86.7% (many diffs)
43 mouse 235106 Ntm neurotrimin XM_006510205.3 78.5% 84.1% (many diffs)
44 mouse 235106 Ntm neurotrimin NM_172290.3 78.4% 81.1% (many diffs)
45 mouse 235106 Ntm neurotrimin XM_017313304.1 76.3% 79% (many diffs)
46 mouse 235106 Ntm neurotrimin XM_017313305.1 76.1% 78.7% (many diffs)
47 mouse 235106 Ntm neurotrimin XM_017313306.1 75.5% 81.6% (many diffs)
48 mouse 235106 Ntm neurotrimin XM_006510206.3 73.9% 76.4% (many diffs)
49 mouse 235106 Ntm neurotrimin XM_006510209.3 71.1% 76.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1014
ORF length:
948
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg ggtctgtggg tacctgttcc tgccctggaa gtgcctcgtg gtcgtgtctc
121tcaggctgct gttccttgta cccacaggag tgcccgtgcg cagcggagat gccaccttcc
181ccaaagctat ggacaacgtg acggtccggc agggggagag cgccaccctc aggtgcacta
241ttgacaaccg ggtcacccgg gtggcctggc taaaccgcag caccatcctc tatgctggga
301atgacaagtg gtgcctggat cctcgcgtgg tccttctgag caacacccaa acgcagtaca
361gcatcgagat ccagaacgtg gatgtgtatg acgagggccc ttacacctgc tcggtgcaga
421cagacaacca cccaaagacc tctagggtcc acctcattgt gcaagtatct cccaaaattg
481tagagatttc ttcagatatc tccattaatg aagggaacaa tattagcctc acctgcatag
541caactggtag accagagcct acggttactt ggagacacat ctctcccaaa gcggttggct
601ttgtgagtga agacgaatac ttGGAAATTC AGGGCATCAC CCGGGAGCAG TCAGGGGACT
661ACGAGTGCAG TGCCTCCAAT GACGTGGCCG CGCCCGTGGT ACGGAGAGTA AAGGTCACCG
721TGAACTATCC ACCATACATT TCAGAAGCCA AGGGTACAGG TGTCCCCGTG GGACAAAAGG
781GGACACTGCA GTGTGAAGCC TCAGCAGTCC CCTCAGCAGA ATTCCAGTGG TACAAGGATG
841ACAAAAGACT GATTGAAGGA AAGAAAGGGG TGAAAGTGGA AAACAGACCT TTCCTCTCAA
901AACTCATCTT CTTCAATGTC TCTGAACATG ACTATGGGAA CTACACTTGC GTGGCCTCCA
961ACAAGCTGGG CCACACCAAT GCCAGCATCA TGCTATTTGG TGAGACTGTG CTCTGCCCAA
1021CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1081ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
1141TATCTTGTGG AAAGGACGAT GAACTCCATT TGGGGCGGGA ACCACGCGTT AAGTCgacaa
1201tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)