Construct: ORF ccsbBroad304_07353

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000749.1_s304c1, BRDN0000406846
Derived from:
ccsbBroadEn_07353
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PIAS2 (9063)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07353
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9063 PIAS2 protein inhibitor of activa... NM_173206.4 99.9% 99.8% 18G>T
2 human 9063 PIAS2 protein inhibitor of activa... NM_001324051.2 98.4% 98.4% 0_1ins27
3 human 9063 PIAS2 protein inhibitor of activa... NM_001324052.2 98.4% 98.4% 0_1ins27
4 human 9063 PIAS2 protein inhibitor of activa... NM_001324053.2 98.4% 98.4% 0_1ins27
5 human 9063 PIAS2 protein inhibitor of activa... NM_001324054.2 98.4% 98.4% 0_1ins27
6 human 9063 PIAS2 protein inhibitor of activa... NM_001354033.2 98.4% 98.4% 0_1ins27
7 human 9063 PIAS2 protein inhibitor of activa... XM_017026072.1 98.4% 98.4% 0_1ins27
8 human 9063 PIAS2 protein inhibitor of activa... XM_006722573.2 93.3% 91.8% (many diffs)
9 human 9063 PIAS2 protein inhibitor of activa... NM_001324046.2 92.4% 93.7% (many diffs)
10 human 9063 PIAS2 protein inhibitor of activa... NM_001354034.2 90.8% 88.7% (many diffs)
11 human 9063 PIAS2 protein inhibitor of activa... XM_005258377.3 89.7% 87.2% (many diffs)
12 human 9063 PIAS2 protein inhibitor of activa... XM_011526258.2 89.5% 87.9% (many diffs)
13 human 9063 PIAS2 protein inhibitor of activa... NM_001324047.2 89.4% 87.4% (many diffs)
14 human 9063 PIAS2 protein inhibitor of activa... NM_001354038.2 89.4% 87.4% (many diffs)
15 human 9063 PIAS2 protein inhibitor of activa... NM_001354039.2 89.4% 87.4% (many diffs)
16 human 9063 PIAS2 protein inhibitor of activa... XM_024451285.1 89.2% 88.1% (many diffs)
17 human 9063 PIAS2 protein inhibitor of activa... NM_004671.5 88.3% 88.8% (many diffs)
18 human 9063 PIAS2 protein inhibitor of activa... NM_001324058.2 88.2% 87.7% (many diffs)
19 human 9063 PIAS2 protein inhibitor of activa... XM_024451286.1 87.7% 86.7% (many diffs)
20 human 9063 PIAS2 protein inhibitor of activa... NM_001324048.2 86.9% 87.6% (many diffs)
21 human 9063 PIAS2 protein inhibitor of activa... NM_001324049.2 86.9% 87.6% (many diffs)
22 human 9063 PIAS2 protein inhibitor of activa... NM_001354035.2 86.9% 87.6% (many diffs)
23 human 9063 PIAS2 protein inhibitor of activa... NM_001354036.2 86.9% 87.6% (many diffs)
24 human 9063 PIAS2 protein inhibitor of activa... XM_024451282.1 86.9% 87.6% (many diffs)
25 human 9063 PIAS2 protein inhibitor of activa... NM_001324055.2 86.7% 86.3% (many diffs)
26 human 9063 PIAS2 protein inhibitor of activa... NM_001324057.2 86.7% 86.3% (many diffs)
27 human 9063 PIAS2 protein inhibitor of activa... NM_001324059.2 86.7% 86.3% (many diffs)
28 human 9063 PIAS2 protein inhibitor of activa... NM_001354037.2 86.7% 86.3% (many diffs)
29 human 9063 PIAS2 protein inhibitor of activa... XM_006722571.3 85.3% 82.1% (many diffs)
30 human 9063 PIAS2 protein inhibitor of activa... XM_024451283.1 83.3% 80.9% (many diffs)
31 human 9063 PIAS2 protein inhibitor of activa... XM_024451284.1 83.3% 80.9% (many diffs)
32 human 9063 PIAS2 protein inhibitor of activa... XM_006722572.3 83% 82.2% (many diffs)
33 human 9063 PIAS2 protein inhibitor of activa... NM_001324060.2 69% 68.4% (many diffs)
34 human 9063 PIAS2 protein inhibitor of activa... XR_001753300.1 59% (many diffs)
35 human 9063 PIAS2 protein inhibitor of activa... XR_001753298.1 57.6% (many diffs)
36 human 9063 PIAS2 protein inhibitor of activa... NR_148699.2 35.8% (many diffs)
37 human 9063 PIAS2 protein inhibitor of activa... NR_136685.2 30.7% (many diffs)
38 human 9063 PIAS2 protein inhibitor of activa... NR_148700.2 15% 1_162del;180G>T;1879_11413del
39 human 9063 PIAS2 protein inhibitor of activa... NR_148701.2 14.8% (many diffs)
40 human 9063 PIAS2 protein inhibitor of activa... NR_148703.2 14.7% (many diffs)
41 human 9063 PIAS2 protein inhibitor of activa... NR_136684.2 14.3% (many diffs)
42 human 9063 PIAS2 protein inhibitor of activa... NR_148702.2 14.3% (many diffs)
43 mouse 17344 Pias2 protein inhibitor of activa... NM_001164169.1 93% 97.5% (many diffs)
44 mouse 17344 Pias2 protein inhibitor of activa... NM_001164170.1 91.8% 96.3% (many diffs)
45 mouse 17344 Pias2 protein inhibitor of activa... NM_001164167.1 87.4% 93.4% (many diffs)
46 mouse 17344 Pias2 protein inhibitor of activa... XM_006526423.2 84.7% 87.1% (many diffs)
47 mouse 17344 Pias2 protein inhibitor of activa... XM_006526425.2 83.6% 86% (many diffs)
48 mouse 17344 Pias2 protein inhibitor of activa... NM_008602.4 82.3% 87.2% (many diffs)
49 mouse 17344 Pias2 protein inhibitor of activa... NM_001164168.1 81.2% 86.1% (many diffs)
50 mouse 17344 Pias2 protein inhibitor of activa... XR_001782348.1 19.1% (many diffs)
51 mouse 17344 Pias2 protein inhibitor of activa... XR_001782349.1 19% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1782
ORF length:
1716
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ggatttcgaa gatttgagga atatggtttc tagttttagg gtttctgaac
121tacaagtatt actaggcttt gctggacgga ataaaagtgg acgcaagcat gacctcctga
181tgagggcgct gcatttattg aagagcggct gcagccctgc ggttcagatt aaaatccgag
241aattgtatag acgccgatat ccacgaactc ttgaaggact ttctgattta tccacaatca
301aatcatcggt tttcagtttg gatggtggct catcacctgt agaacctgac ttggccgtgg
361ctggaatcca ctcgttgcct tccacttcag ttacacctca ctcaccatcc tctcctgttg
421gttctgtgct gcttcaagat actaagccca catttgagat gcagcagcca tctcccccaa
481ttcctcctgt ccatcctgat gtgcagttaa aaaatctgcc cttttatgat gtccttgatg
541ttctcatcaa gcccacgagt ttagttcaaa gcagtattca gcgatttcaa gagaagtttt
601ttatttttgc tttgacacct caacaagtta gagagatatg catatccagg gattttttgc
661caggtggtag gagagattat acagtccaag ttcagttgag actttgcctg gcagagacaa
721gttgccctca agaagataac tatccaaata gtctatgtat aaaagtaaat gggaagctat
781ttcctttgcc tggctatgca ccaccgccta aaaatgggat tgaacagaag cgccctggac
841gccccttgaa tattacatct ttagttaggt tatcttcagc tgtgccaaac caaatttcca
901tttcttgggc atcagaaatt gggaagaatt actctatgtc tgtatatctt gtacggcagc
961ttacatcagc catgttatta cagagattaa aaatgaaagg tattagaaac cctgatcatt
1021ccagagcact aattaaagaa aaacttactg cagatcctga tagtgaaatt gctacaacta
1081gccttcgggt atccttgatg tgccctttag gaaaaatgag gctgacaatc ccatgccgtg
1141cagtgacttg tacacatctg cagtgttttg atgctgccct ctatctacaa atgaatgaga
1201aaaagcccac ctggatttgt cctgtgtgtg acaaaaaagc tgcctatgaa agtctaatat
1261tagatgggct ttttatggaa attctcaatg actgttctga tgtagatgag atcaaattcc
1321aagaagatgg ttcttggtgt ccaatgagac cgaagaaaga agctatgaaa gtatccagcc
1381aaccgtgtac aaaaatagaa agttcaagcg tcctcagtaa gccttgttca gtgactgtag
1441ccagtgaggc aagcaagaag aaagtagatg ttattgatct tacaatagaa agctcttctg
1501acgaagagga agaccctcct gccaaaagga aatgcatctt tatgtcagaa acacaaagca
1561gcccaaccaa aggggttctc atgtatcagc catcttctgt aagggtgccc agtgtgactt
1621cggttgatcc tgctgctatt ccgccttcat taacagacta ctcagtacca ttccaccata
1681cgccaatatc aagcatgtca tcagatttgc caggagaaca aagaagaaat gatattaata
1741atgaactgaa gcttggaaca tcttctgata ctgtgcaaca gtgcccaact ttcttgtaca
1801aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1861ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)