Construct: ORF ccsbBroad304_07648

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005131.1_s304c1, BRDN0000407185
Derived from:
ccsbBroadEn_07648
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDLIM5 (10611)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07648
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10611 PDLIM5 PDZ and LIM domain 5 NM_006457.5 99.8% 99.4% 1033G>A;1141A>G;1475G>A
2 human 10611 PDLIM5 PDZ and LIM domain 5 NM_001011513.4 81.5% 81.2% (many diffs)
3 human 10611 PDLIM5 PDZ and LIM domain 5 NM_001256428.2 79.3% 79% (many diffs)
4 human 10611 PDLIM5 PDZ and LIM domain 5 NM_001256427.2 79% 78.7% (many diffs)
5 human 10611 PDLIM5 PDZ and LIM domain 5 NM_001256426.1 66.2% 65.9% (many diffs)
6 human 10611 PDLIM5 PDZ and LIM domain 5 NM_001256425.1 45.3% 45.1% 0_1ins975;166A>G;500G>A
7 human 10611 PDLIM5 PDZ and LIM domain 5 NM_001011515.2 36.1% 33% (many diffs)
8 human 10611 PDLIM5 PDZ and LIM domain 5 NM_001011516.2 33.8% 29.5% (many diffs)
9 mouse 56376 Pdlim5 PDZ and LIM domain 5 NM_019808.3 85.6% 88.4% (many diffs)
10 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501746.1 84% 86.8% (many diffs)
11 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501743.1 78% 80.4% (many diffs)
12 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501741.1 73.9% 75.3% (many diffs)
13 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501742.1 73.5% 75.6% (many diffs)
14 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501740.1 71.2% 72.2% (many diffs)
15 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501739.1 70.6% 71.6% (many diffs)
16 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501738.1 70.1% 71.1% (many diffs)
17 mouse 56376 Pdlim5 PDZ and LIM domain 5 NM_001190854.1 69.4% 72.9% (many diffs)
18 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501748.1 68.7% 72.2% (many diffs)
19 mouse 56376 Pdlim5 PDZ and LIM domain 5 NM_001190855.1 64.7% 67.8% (many diffs)
20 mouse 56376 Pdlim5 PDZ and LIM domain 5 NM_001190856.1 61% 63.2% (many diffs)
21 mouse 56376 Pdlim5 PDZ and LIM domain 5 NM_001190852.1 57.9% 59.6% (many diffs)
22 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501745.1 57.4% 59.1% (many diffs)
23 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501744.1 57% 58.7% (many diffs)
24 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_017319659.1 55.8% 56.8% (many diffs)
25 mouse 56376 Pdlim5 PDZ and LIM domain 5 NM_019809.3 49.2% 46.2% (many diffs)
26 mouse 56376 Pdlim5 PDZ and LIM domain 5 NM_001190857.1 32.8% 30.7% (many diffs)
27 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501751.2 32.8% 30.7% (many diffs)
28 mouse 56376 Pdlim5 PDZ and LIM domain 5 NM_022554.3 32.5% 30.5% (many diffs)
29 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501750.2 32.5% 30.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1854
ORF length:
1788
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag caactacagt gtgtcactgg ttggcccagc tccttggggt ttccggctgc
121agggcggtaa ggatttcaac atgcctctga caatctctag tctaaaagat ggcggcaagg
181cagcccaggc aaatgtaaga ataggcgatg tggttctcag cattgatgga ataaatgcac
241aaggaatgac tcatcttgaa gcccagaata agattaaggg ttgtacaggc tctttgaata
301tgactctgca aagagcatct gctgcaccca agcctgagcc ggttcctgtt caaaagggag
361aacctaaaga agtagttaaa cctgtgccca ttacatctcc tgctgtgtcc aaagtcactt
421ccacaaacaa catggcctac aataaggcac cacggccttt tggttctgtg tcttcaccaa
481aagtcacatc catcccatca ccatcgtctg ccttcacccc agcccatgcg accacctcat
541cacatgcttc cccttcaccc gtggctgccg tcactcctcc cctgttcgct gcatctggac
601tgcatgctaa tgccaatctt agtgctgacc agtctccatc tgcactgagc gctggtaaaa
661ctgcagttaa tgtcccacgg cagcccacag tcaccagcgt gtgttccgag acttctcagg
721agctagcaga gggacagaga agaggatccc agggtgacag taaacagcaa aatggcccac
781caagaaaaca cattgtggag cgctatacag agttttatca tgtacccact cacagtgatg
841ccagcaagaa gagactgatt gaggatactg aagactggcg tccaaggact ggaacaactc
901agtctcgctc tttccgaatc cttgcccaga tcactgggac tgaacatttg aaagaatctg
961aagccgataa tacaaagaag gcaaataact ctcaggagcc ttctccgcag ttggcttcct
1021cggtagcttc cacacggagc atgcccgaga gcctggacag cccaacctct ggcagaccag
1081gggttaccag cctcacaact gcagctgcct tcaagcctgt aggatccact ggcgtcatca
1141agtcaccaag ctggcaacgg ccaaaccaag gagtaccttc cactggaaga atctcaaaca
1201gcgctgctta ctcaggatca gtggcaccag ccaactcagc tttgggacaa acccagccaa
1261gtgaccagga cactttagtg caaagagctg agcacattcc agcagggaaa cgaactccga
1321tgtgcgccca ttgtaaccag gtcatcagag gaccattctt agtggcactg gggaaatctt
1381ggcacccaga agaattcaac tgcgctcact gcaaaaatac aatggcctac attggatttg
1441tagaggagaa aggagccctg tattgtgagc tgtgctatga gaaattcttt gcccctgaat
1501gtggtcgatg ccaaaggaag atccttggag aagtcatcaa tgcgttgaaa caaacttggc
1561atgtttcctg ttttgtgtgt gtagcctgtg gaaagcccat tcggaacaat gtttttcact
1621tggaggatgg tgaaccctac tgtgagactg attattatgc cctctttggt actatatgcc
1681atggatgtga atttcccata gaagctggtg acatgttcct ggaagctctg ggctacacct
1741ggcatgacac ttgctttgta tgctcagtgt gttgtgaaag tttggaaggt cagacctttt
1801tctccaagaa ggacaagccc ctgtgtaaga aacatgctca ttctgtgaat ttttgcccaa
1861ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
1921cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.