Construct: ORF ccsbBroad304_07648

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005131.1_s304c1, BRDN0000407185
Derived from:
ccsbBroadEn_07648
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDLIM5 (10611)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07648
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10611 PDLIM5 PDZ and LIM domain 5 NM_006457.5 99.8% 99.4% 1033G>A;1141A>G;1475G>A
2 human 10611 PDLIM5 PDZ and LIM domain 5 NM_001011513.4 81.5% 81.2% (many diffs)
3 human 10611 PDLIM5 PDZ and LIM domain 5 NM_001256428.2 79.3% 79% (many diffs)
4 human 10611 PDLIM5 PDZ and LIM domain 5 NM_001256427.2 79% 78.7% (many diffs)
5 human 10611 PDLIM5 PDZ and LIM domain 5 NM_001256426.1 66.2% 65.9% (many diffs)
6 human 10611 PDLIM5 PDZ and LIM domain 5 NM_001256425.1 45.3% 45.1% 0_1ins975;166A>G;500G>A
7 human 10611 PDLIM5 PDZ and LIM domain 5 NM_001011515.2 36.1% 33% (many diffs)
8 human 10611 PDLIM5 PDZ and LIM domain 5 NM_001011516.2 33.8% 29.5% (many diffs)
9 mouse 56376 Pdlim5 PDZ and LIM domain 5 NM_019808.3 85.6% 88.4% (many diffs)
10 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501746.1 84% 86.8% (many diffs)
11 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501743.1 78% 80.4% (many diffs)
12 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501741.1 73.9% 75.3% (many diffs)
13 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501742.1 73.5% 75.6% (many diffs)
14 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501740.1 71.2% 72.2% (many diffs)
15 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501739.1 70.6% 71.6% (many diffs)
16 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501738.1 70.1% 71.1% (many diffs)
17 mouse 56376 Pdlim5 PDZ and LIM domain 5 NM_001190854.1 69.4% 72.9% (many diffs)
18 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501748.1 68.7% 72.2% (many diffs)
19 mouse 56376 Pdlim5 PDZ and LIM domain 5 NM_001190855.1 64.7% 67.8% (many diffs)
20 mouse 56376 Pdlim5 PDZ and LIM domain 5 NM_001190856.1 61% 63.2% (many diffs)
21 mouse 56376 Pdlim5 PDZ and LIM domain 5 NM_001190852.1 57.9% 59.6% (many diffs)
22 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501745.1 57.4% 59.1% (many diffs)
23 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501744.1 57% 58.7% (many diffs)
24 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_017319659.1 55.8% 56.8% (many diffs)
25 mouse 56376 Pdlim5 PDZ and LIM domain 5 NM_019809.3 49.2% 46.2% (many diffs)
26 mouse 56376 Pdlim5 PDZ and LIM domain 5 NM_001190857.1 32.8% 30.7% (many diffs)
27 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501751.2 32.8% 30.7% (many diffs)
28 mouse 56376 Pdlim5 PDZ and LIM domain 5 NM_022554.3 32.5% 30.5% (many diffs)
29 mouse 56376 Pdlim5 PDZ and LIM domain 5 XM_006501750.2 32.5% 30.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1854
ORF length:
1788
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag caactacagt gtgtcactgg ttggcccagc tccttggggt ttccggctgc
121agggcggtaa ggatttcaac atgcctctga caatctctag tctaaaagat ggcggcaagg
181cagcccaggc aaatgtaaga ataggcgatg tggttctcag cattgatgga ataaatgcac
241aaggaatgac tcatcttgaa gcccagaata agattaaggg ttgtacaggc tctttgaata
301tgactctgca aagagcatct gctgcaccca agcctgagcc ggttcctgtt caaaagggag
361aacctaaaga agtagttaaa cctgtgccca ttacatctcc tgctgtgtcc aaagtcactt
421ccacaaacaa catggcctac aataaggcac cacggccttt tggttctgtg tcttcaccaa
481aagtcacatc catcccatca ccatcgtctg ccttcacccc agcccatgcg accacctcat
541cacatgcttc cccttcaccc gtggctgccg tcactcctcc cctgttcgct gcatctggac
601tgcatgctaa tgccaatctt agtgctgacc agtctccatc tgcactgagc gctggtaaaa
661ctgcagttaa tgtcccacgg cagcccacag tcaccagcgt gtgttccgag acttctcagg
721agctagcaga gggacagaga agaggatccc agggtgacag taaacagcaa aatggcccac
781caagaaaaca cattgtggag cgctatacag agttttatca tgtacccact cacagtgatg
841ccagcaagaa gagactgatt gaggatactg aagactggcg tccaaggact ggaacaactc
901agtctcgctc tttccgaatc cttgcccaga tcactgggac tgaacatttg aaagaatctg
961aagccgataa tacaaagaag gcaaataact ctcaggagcc ttctccgcag ttggcttcct
1021cggtagcttc cacacggagc atgcccgaga gcctggacag cccaacctct ggcagaccag
1081gggttaccag cctcacaact gcagctgcct tcaagcctgt aggatccact ggcgtcatca
1141agtcaccaag ctggcaacgg ccaaaccaag gagtaccttc cactggaaga atctcaaaca
1201gcgctgctta ctcaggatca gtggcaccag ccaactcagc tttgggacaa acccagccaa
1261gtgaccagga cactttagtg caaagagctg agcacattcc agcagggaaa cgaactccga
1321tgtgcgccca ttgtaaccag gtcatcagag gaccattctt agtggcactg gggaaatctt
1381ggcacccaga agaattcaac tgcgctcact gcaaaaatac aatggcctac attggatttg
1441tagaggagaa aggagccctg tattgtgagc tgtgctatga gaaattcttt gcccctgaat
1501gtggtcgatg ccaaaggaag atccttggag aagtcatcaa tgcgttgaaa caaacttggc
1561atgtttcctg ttttgtgtgt gtagcctgtg gaaagcccat tcggaacaat gtttttcact
1621tggaggatgg tgaaccctac tgtgagactg attattatgc cctctttggt actatatgcc
1681atggatgtga atttcccata gaagctggtg acatgttcct ggaagctctg ggctacacct
1741ggcatgacac ttgctttgta tgctcagtgt gttgtgaaag tttggaaggt cagacctttt
1801tctccaagaa ggacaagccc ctgtgtaaga aacatgctca ttctgtgaat ttttgcccaa
1861ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
1921cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)