Construct: ORF ccsbBroad304_08342

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009553.1_s304c1, BRDN0000462401
Derived from:
ccsbBroadEn_08342
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RAPGEF6 (51735)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08342
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51735 RAPGEF6 Rap guanine nucleotide exch... NM_001164388.2 99.9% 100% 252G>A
2 human 51735 RAPGEF6 Rap guanine nucleotide exch... NM_001164387.2 99.6% 99.6% 252G>A;2240_2254del
3 human 51735 RAPGEF6 Rap guanine nucleotide exch... NM_001164386.2 93% 92.8% (many diffs)
4 human 51735 RAPGEF6 Rap guanine nucleotide exch... NM_016340.6 92.5% 92.3% (many diffs)
5 human 51735 RAPGEF6 Rap guanine nucleotide exch... NM_001164389.2 88.8% 84.7% (many diffs)
6 human 51735 RAPGEF6 Rap guanine nucleotide exch... NM_001164390.2 54.9% 54.9% 252G>A;2481_2482ins2031
7 mouse 192786 Rapgef6 Rap guanine nucleotide exch... XM_006532564.3 89% 91.7% (many diffs)
8 mouse 192786 Rapgef6 Rap guanine nucleotide exch... NM_001252496.1 88.7% 91.4% (many diffs)
9 mouse 192786 Rapgef6 Rap guanine nucleotide exch... XM_017314348.1 88.2% 90.8% (many diffs)
10 mouse 192786 Rapgef6 Rap guanine nucleotide exch... XM_006532561.3 83.1% 85.4% (many diffs)
11 mouse 192786 Rapgef6 Rap guanine nucleotide exch... XM_006532560.3 82.9% 85.1% (many diffs)
12 mouse 192786 Rapgef6 Rap guanine nucleotide exch... NM_175258.4 82.6% 84.9% (many diffs)
13 mouse 192786 Rapgef6 Rap guanine nucleotide exch... NM_001252494.1 82.4% 84.6% (many diffs)
14 mouse 192786 Rapgef6 Rap guanine nucleotide exch... XM_006532567.3 80.6% 78.7% (many diffs)
15 mouse 192786 Rapgef6 Rap guanine nucleotide exch... XM_006532568.3 80.4% 78.5% (many diffs)
16 mouse 192786 Rapgef6 Rap guanine nucleotide exch... XM_006532562.2 80.2% 82.2% (many diffs)
17 mouse 192786 Rapgef6 Rap guanine nucleotide exch... XM_006532563.3 77.5% 78.6% (many diffs)
18 mouse 192786 Rapgef6 Rap guanine nucleotide exch... XM_006532566.3 74.8% 75.2% (many diffs)
19 mouse 192786 Rapgef6 Rap guanine nucleotide exch... XM_006532565.3 74.5% 74.9% (many diffs)
20 mouse 192786 Rapgef6 Rap guanine nucleotide exch... NM_001252497.1 36.1% 36.2% (many diffs)
21 mouse 192786 Rapgef6 Rap guanine nucleotide exch... NM_001252498.1 25.9% 25.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
4581
ORF length:
4512
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaactcaccc gtggaccctg gcgctaggca ggcgttgagg aagaagccac
121ccgagcggac tcccgaggac ttaaatacta tttattctta tcttcatgga atggaaatat
181tatcaaatct cagggaacat cagcttagat taatgtctgc aagagcacgc tatgagagat
241acagtggcaa tcaggttctc ttttgttcag aaacgattgc cagatgttgg tatatcctac
301tttctggatc tgtgcttgta aaaggctcca tggtcttgcc tccttgcagt tttggtaagc
361agtttggagg aaaaagagga tgtgattgtc ttgtattaga gccttcagaa atgattgtgg
421tagagaatgc caaagataat gaagatagta ttctacaaag agaaattcct gccagacaat
481cccgaagaag atttcggaaa attaactata aaggagagcg ccaaaccatt actgatgatg
541tggaggttaa cagctatctt tctcttccag ctgatcttac caagatgcat ctcacagaaa
601accctcatcc acaggtgact catgtgtctt ctagtcagtc tggttgtagc attgccagtg
661actctggaag cagcagttta tctgatatct atcaggctac ggagagtgag gtaggagatg
721tagatttgac acgtcttcca gaaggacctg ttgattctga ggatgacgaa gaggaagatg
781aagagattga tcgaacagat ccattgcagg ggcgagatct tgttcgagaa tgtcttgaaa
841aagaacctgc agacaaaact gatgatgaca ttgaacaatt gctggagttt atgcaccagc
901tccctgcatt tgcaaacatg accatgtctg taaggagaga actctgctca gtgatgattt
961ttgaagtggt agagcaggct ggagctatta ttcttgaaga tgggcaagag cttgactcat
1021ggtatgttat tttaaacggc actgtggaaa tcagtcatcc agatggaaaa gttgaaaatt
1081tgtttatggg aaatagtttt ggaattactc ccactctgga taagcagtac atgcatggaa
1141ttgtcaggac taaagtagat gattgtcagt ttgtctgcat agcccagcaa gattattgga
1201gaattttaaa ccatgtggaa aaaaataccc ataaagttga ggaagaggga gaaattgtta
1261tggtacatga gcatcgggaa ctagaccgga gtggaaccag gaaaggacac attgtgatca
1321aggcaacacc tgagcgtctc ataatgcatt taatagaaga acattccatc gtggatccaa
1381cttatataga agattttcta ttaacttaca ggacatttct tgaaagtcct ttggatgttg
1441ggatcaaact attggaatgg tttaagatcg acagcttaag agataaggtg acacggattg
1501tattattatg ggtaaataat cattttaatg attttgaagg tgaccctgct atgactcgat
1561ttctagagga atttgaaaaa aatctggaag atacaaagat gaatggtcat ctccggttat
1621tgaatattgc ctgtgctgca aaggctaagt ggagacaggt tgtgctgcaa aaggcttccc
1681gcgagtcccc tctacaattc agccttaatg gagggagtga gaagggattt ggtatttttg
1741ttgaaggagt agaacctggt agcaaagctg ctgattcagg actgaaacgt ggtgatcaga
1801ttatggaagt aaatggacaa aactttgaga atattacatt tatgaaagcc gttgaaattt
1861tgaggaataa tactcatctt gcacttactg tgaagaccaa catttttgtg ttcaaagagt
1921tactttttag gactgaacaa gagaaatctg gtgttcctca tattcccaaa attgctgaaa
1981aaaaaagtaa tcgccattct atccagcatg tgccaggaga tattgaacag acatcacagg
2041agaaaggaag taagaaagtt aaagcaaata ctgtttcagg tggaagaaac aaaatcagga
2101agattttgga taaaacacga tttagtatct tgcctccaaa gctatttagt gatggaggcc
2161taagccaatc acaagatgac agcattgtgg gaacaaggca ctgtaggcat agtctggcta
2221taatgcccat ccctggaaca ctctcatcca gcagccctga tctcctgcag cctaccacca
2281gtatgttgga tttttccaat ccttcagata tccctgatca agttataaga gttttcaaag
2341tggatcagca aagttgctac attatcatca gtaaagacac cacagctaaa gaagtagttt
2401ttcatgctgt tcatgaattt ggtttgaccg gtgcatccga cacatattct ctctgtgaag
2461tttctgttac tcctgagggt gtcataaaac agagaagact tccagatcag ttctccaaat
2521tagctgatag aattcaactc aatggaaggt attacttaaa aaataacatg gaaacagaaa
2581ccttatgttc agatgaagat gctcaagaac tagttaagga aagccagcta tccatgctgc
2641agctcagtac cattgaggtg gccacccagc tgtcaatgag ggactttgat ttgtttcgta
2701atattgaacc gactgagtac atcgatgacc tttttaagtt aaattccaaa acaggaaata
2761ctcatttgaa gaggtttgag gacattgtaa accaagagac attctgggtt gcctcagaaa
2821ttttaactga agcaaatcag ctcaaacgaa tgaagattat taagcatttt attaaaattg
2881cacttcattg tcgagaatgt aagaacttca attccatgtt tgcaataata agtggcttga
2941acctggcatc tgtagcaaga ctcagaggaa cttgggaaaa gttaccaagc aaatacgaga
3001aacatcttca agatctacaa gacatttttg atccatctag aaacatggca aagtatagaa
3061atattcttag tagtcaaagt atgcagcctc caattattcc actcttccct gttgtcaaga
3121aagatatgac atttctacat gaaggaaatg actccaaagt agatggttta gtaaactttg
3181agaagttaag aatgatttcc aaggaaatcc gccaagttgt tcgaatgact tctgctaaca
3241tggacccagc tatgatgttt cgacagagga agaagaggtg gcggagtctg gggtcactga
3301gtcaaggaag cacaaattca aacatgctgg atgttcaggg aggtgctcac aaaaaaaggg
3361cacgccgcag ctctctgctt aatgccaaga agctatatga ggatgcccaa atggcaagga
3421aggtgaagca gtatctttcc agtctcgatg tagagacaga tgaggagaag ttccagatga
3481tgtcattaca gtgggagcct gcatatggta ccttgaccaa gaatttaagt gagaaaagat
3541cagccaaatc atctgaaatg tctccagtgc ctatgaggtc agctggccaa acaactaaag
3601cccacttgca tcaaccccac agagtaagcc aggtgcttca ggtgccagct gttaatttgc
3661accccatcag gaagaaggga caaacaaaag accctgcact gaatacaagt ttacctcaga
3721aagttttagg aacaactgaa gaaataagtg gtaagaagca tacagaagac actatttctg
3781tggcgtcatc tttacattct agtcctcctg catctcctca aggctcccct cacaaaggtt
3841acacacttat tccatcagct aaatctgaca acttgtctga ctccagccat agtgagattt
3901cttcacggtc cagcatcgtg agcaattgtt ctgttgactc catgtctgca gctctacagg
3961atgaacggtg ttcctctcag gccctggcag tccctgaatc cactggggca ttggaaaaga
4021cagagcacgc ttcagggata ggagatcata gtcaacatgg ccctgggtgg acactcttga
4081agccatctct aatcaagtgt ttagctgtct catcgtctgt gagcaatgaa gagatttctc
4141aagagcatat cattatagaa gcagctgaca gtggtcgtgg aagttggact tcgtgttcaa
4201gcagctccca tgacaacttc caaagccttc caaacccaaa aagctgggat tttttgaact
4261cttacagaca tacccatttg gatgacccca ttgctgaagt tgaacccact gactctgagc
4321cctattcctg ttctaaaagc tgctctagaa cttgtgggca gtgtaaagga agcctagaga
4381gaaagagttg gacctcctcc agttctctgt ctgacacgta tgaaccaaac tatgggacag
4441ttaaacagag agtattggag agcaccccag ctgagtcatc tgaaggcttg gaccccaagg
4501atgccactga cccagtttat aaaactgtca cttcaagtac agaaaagggc ttgattgaaa
4561atgaacaagt ttcagcagtc ttgccaactt tcttgtacaa agtggttggt aagcctatcc
4621ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
4681ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)