Transcript: Human NM_001321524.1

Homo sapiens zinc finger protein 845 (ZNF845), transcript variant 4, mRNA.

Source:
NCBI, updated 2018-07-01
Taxon:
Homo sapiens (human)
Gene:
ZNF845 (91664)
Length:
5693
CDS:
250..3162

Additional Resources:

NCBI RefSeq record:
NM_001321524.1
NBCI Gene record:
ZNF845 (91664)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001321524.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000236604 AGACTTCCAATCCATCAATAT pLKO_005 4180 3UTR 100% 13.200 18.480 N ZNF845 n/a
2 TRCN0000236605 ACCTCCTTAATTCAGTTTATT pLKO_005 4259 3UTR 100% 15.000 10.500 N ZNF845 n/a
3 TRCN0000236601 ATGGGTTGTATGTCTATTTAG pLKO_005 4199 3UTR 100% 13.200 9.240 N ZNF845 n/a
4 TRCN0000236603 TGGGTATCTGTTGAATCTAAA pLKO_005 4125 3UTR 100% 13.200 9.240 N ZNF845 n/a
5 TRCN0000236602 ATGAATGCAGCAGGACCTTTA pLKO_005 1406 CDS 100% 10.800 7.560 N ZNF845 n/a
6 TRCN0000021905 GACGTGATGCTGGAGAATTAT pLKO.1 352 CDS 100% 15.000 7.500 Y ZNF765 n/a
7 TRCN0000018107 GCTGGAAACAAGCCTATTAAA pLKO.1 640 CDS 100% 15.000 7.500 Y ZNF600 n/a
8 TRCN0000021906 CCCTGCTCAGAGGACTCTATA pLKO.1 327 CDS 100% 13.200 6.600 Y ZNF765 n/a
9 TRCN0000148017 GAGAAACCTTACAGGTGTAAT pLKO.1 2227 CDS 100% 13.200 6.600 Y ZNF813 n/a
10 TRCN0000149130 GCTGGAGAGAAACCTTACAAA pLKO.1 3561 3UTR 100% 5.625 2.813 Y ZNF761 n/a
11 TRCN0000142234 GTCAGATGTCATCCCTTGTAT pLKO.1 1511 CDS 100% 5.625 2.813 Y ZNF468 n/a
12 TRCN0000015887 GTGAAGAATGTGACAAAGTTT pLKO.1 2495 CDS 100% 5.625 2.813 Y ZNF702P n/a
13 TRCN0000021908 TCAGGGATGTGGCCATAGAAT pLKO.1 278 CDS 100% 5.625 2.813 Y ZNF765 n/a
14 TRCN0000150044 CCTTGAAAGACATAGGAGAAT pLKO.1 1608 CDS 100% 4.950 2.475 Y ZNF816 n/a
15 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 3982 3UTR 100% 4.950 2.475 Y ERAP2 n/a
16 TRCN0000015885 GCAATTCATACTGGAGAGAAA pLKO.1 1204 CDS 100% 4.950 2.475 Y ZNF702P n/a
17 TRCN0000150214 GTAATGAATGTGGCAAGGTTT pLKO.1 3083 CDS 100% 4.950 2.475 Y ZNF813 n/a
18 TRCN0000148307 CAATGTAATGAGAGTGGCAAA pLKO.1 895 CDS 100% 4.050 2.025 Y ZNF321P n/a
19 TRCN0000021904 GACTCTATACAGGGACGTGAT pLKO.1 339 CDS 100% 4.050 2.025 Y ZNF765 n/a
20 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 4021 3UTR 100% 4.050 2.025 Y P3H4 n/a
21 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 4021 3UTR 100% 4.050 2.025 Y ORAI2 n/a
22 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 4021 3UTR 100% 4.050 2.025 Y P3H4 n/a
23 TRCN0000021907 GCTGGAGAATTATAGGAACCT pLKO.1 360 CDS 100% 2.640 1.320 Y ZNF765 n/a
24 TRCN0000235273 ACTGGAGAGAAACCTTATAAA pLKO_005 1465 CDS 100% 15.000 7.500 Y Gm10771 n/a
25 TRCN0000337271 ACTGGAGAGAAACCTTATAAA pLKO_005 1465 CDS 100% 15.000 7.500 Y ZNF286B n/a
26 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 3983 3UTR 100% 13.200 6.600 Y LIAS n/a
27 TRCN0000021816 CTGGAGAGAAACCTTACAGAT pLKO.1 2222 CDS 100% 4.950 2.475 Y ZNF253 n/a
28 TRCN0000155654 CTGGAGAGAAACCTTACAGAT pLKO.1 2222 CDS 100% 4.950 2.475 Y ZNF320 n/a
29 TRCN0000158848 GAGAAACCTTACAAATGTGAT pLKO.1 1303 CDS 100% 4.950 2.475 Y ZNF28 n/a
30 TRCN0000147979 GCAATTCATACTGGAGAGAAT pLKO.1 1204 CDS 100% 4.950 2.475 Y ZNF321P n/a
31 TRCN0000148611 CGTAGACTTCATACTGGAGAA pLKO.1 1285 CDS 100% 4.050 2.025 Y ZNF761 n/a
32 TRCN0000149655 GCCCTTGTAATTCATAAGGCT pLKO.1 1186 CDS 100% 0.750 0.375 Y ZNF813 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001321524.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04800 pDONR223 100% 55.5% 47.5% None (many diffs) n/a
2 ccsbBroad304_04800 pLX_304 0% 55.5% 47.5% V5 (many diffs) n/a
3 TRCN0000478315 CGTGCCTTGTCGAAGCCACGTTAA pLX_317 18.1% 55.5% 47.5% V5 (many diffs) n/a
4 ccsbBroadEn_08581 pDONR223 100% 41.8% 37.1% None (many diffs) n/a
5 ccsbBroad304_08581 pLX_304 0% 41.8% 37.1% V5 (many diffs) n/a
6 TRCN0000476486 AGTATTTAAATCGATGTCTATAAC pLX_317 30.4% 41.8% 37.1% V5 (many diffs) n/a
7 ccsbBroadEn_05629 pDONR223 100% 15.4% 13.5% None (many diffs) n/a
8 ccsbBroad304_05629 pLX_304 0% 15.4% 13.5% V5 (many diffs) n/a
9 TRCN0000468214 TCTCTAGTACCTCAATAGGTGGTT pLX_317 94.7% 15.4% 13.5% V5 (many diffs) n/a
10 ccsbBroadEn_14339 pDONR223 100% 5.7% .2% None (many diffs) n/a
11 ccsbBroad304_14339 pLX_304 0% 5.7% .2% V5 (not translated due to prior stop codon) (many diffs) n/a
12 TRCN0000478280 ACGAGTTCACTGTCATGACCAACC pLX_317 100% 5.7% .2% V5 (not translated due to prior stop codon) (many diffs) n/a
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